Strain Name:
FVB/N-Tg(tetO-HTR4*D100A)7Niss/Mmmh
Stock Number:
029992-MU
Citation ID:
RRID:MMRRC_029992-MU

Strain Information

tetOp
Name: tetracycline operon
Type: Gene
Species: Synthetic
Chromosome:
HTR4
Name: 5-hydroxytryptamine (serotonin) receptor 4 - D100A
Type: Gene
Species: Homo sapiens (human)
Chromosome: 5
Tg(tetO-HTR4*D100A)7Niss
Name: transgene insertion 7, Robert A Nissenson
Synonyms: TetO-Rs1
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
This is a transgenic mouse line carrying the TetO-Rs1 transgene. Rs1 is an engineered G-protein coupled receptor based on the human 5HT4b serotonin receptor but with a D100A mutation that renders it insensitive to serotonin. Rs1 is linked to the TetO tetracycline transactivator promoter. Tetracycline-dependent regulation of Rs1 expression is achieved when this TetO-Rs1 mouse line is mated to a second transgenic line carrying a tTA or rtTA transactivator protein.
Genotype Determination
Phenotype
Homozygous: Does not appear to have a gross phenotype. Does appear to have normal fertility and lifespan.

Hemizygous: No identified phenotype as a single transgenic.
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Bone Density/physiology
  • Cell Line
  • Female
  • GTP-Binding Protein alpha Subunits, Gs/biosynthesis
  • GTP-Binding Protein alpha Subunits, Gs/genetics
  • GTP-Binding Protein alpha Subunits, Gs/metabolism
  • GTP-Binding Protein alpha Subunits, Gs/physiology
  • Humans
  • Ligands
  • Male
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Osteoblasts/chemistry
  • Osteoblasts/metabolism
  • Protein Engineering/methods
  • Receptors, Serotonin, 5-HT4/biosynthesis
  • Receptors, Serotonin, 5-HT4/genetics
  • Serotonin 5-HT4 Receptor Agonists
  • Signal Transduction/genetics
  • Signal Transduction/physiology
Strain Development
This transgenic mouse line was generated by pronuclear injection of a construct carrying the TetO-Rs1 transgenic construct. Mice have been maintained on a FVBN background.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Endocrine Deficiency
  • Models for Human Disease
  • Neurobiology
  • Research Tools
Donor
Bruce Conklin, Ph.D., Gladstone Institute of Cardiovascular Disease
Primary Reference
Hsiao EC; Boudignon BM; Chang WC; Bencsik M; Peng J; Nguyen TD; Manalac C; Halloran BP; Conklin BR; Nissenson RA, Osteoblast expression of an engineered Gs-coupled receptor dramatically increases bone mass., Proc Natl Acad Sci U S A 2008 Jan 29;105(4):1209-14 (Medline PMID: 18212126)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
029992-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
029992-MU-RESUS Litter recovered from cryo-archive $2,697.00 / $5,488.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.