Strain Name:
C57BL/6J-Tlr9m3Btlr/Mmucd
Stock Number:
030343-UCD
Citation ID:
RRID:MMRRC_030343-UCD
Other Names:
CpG3, C57BL/6J-Tlr9m3Btlr/Mmcd
Major Collection:

Strain Information

Tlr9m3Btlr
Name: toll-like receptor 9; mutation 3, Bruce Beutler
Synonyms: Cpg3, Tlr9CpG3, unmethylated CpG-oligodeoxynucleotide unresponsive 3
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
Tlr9
Name: toll-like receptor 9
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 81897
Homologene: 68126
Genetic Alterations
ENU-induced T to A transversion at position 747 of Tlr9 gene (Genbank Accession NM_031178); results in valine to glutamic acid change at position 214 of the TLR9 protein.
Genotype Determination
Phenotype
Homozygous phenotype: The CpG3 phenotype was identified in a G3 screen for mutants with impaired response to Toll- like receptor (TLR) ligands. Peritoneal macrophages from homozygous CpG3 mice produce normal amounts of tumor necrosis factor (TNF)-alpha in response to all TLR ligands tested, except oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs). In response to CpG ODN treatment, homozygous CpG3 macrophages produce no TNF-alpha. In addition, na
Mammalian Phenotype Terms
Allelic Composition: Tlr9m3Btlr/Tlr9m3Btlr (Genetic Background: C57BL/6J-Tlr9m3Btlr )

  • immune system
  • hematopoietic system
MeSH Terms
  • Animals
  • Ethylnitrosourea
  • Genetic Predisposition to Disease
  • Herpesviridae Infections/genetics
  • Herpesviridae Infections/immunology
  • Interferon Type I/antagonists & inhibitors
  • Interferon Type I/biosynthesis
  • Listeriosis/genetics
  • Listeriosis/immunology
  • MAP Kinase Kinase Kinases/genetics
  • MAP Kinase Kinase Kinases/physiology
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Muromegalovirus/immunology
  • Mutagenesis/immunology
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/physiology
  • RNA Splicing/genetics
  • Signal Transduction/genetics
  • Signal Transduction/immunology
  • Streptococcal Infections/enzymology
  • Streptococcal Infections/genetics
  • Streptococcal Infections/immunology
  • Streptococcus agalactiae/immunology
Strain Development
Original mutant is a C57BL/6J G3 ENU- induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Immunology and Inflammation
  • Virology
Donor
Bruce Beutler, M.D., The Scripps Research Institute
Primary Reference
  • Xiao N; Eidenschenk C; Krebs P; Brandl K; Blasius AL; Xia Y; Khovananth K; Smart NG; Beutler B, The Tpl2 mutation Sluggish impairs type I IFN production and increases susceptibility to group B streptococcal disease., J Immunol 2009 Dec 15;183(12):7975-83 (Medline PMID: 19923465)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030343-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030343-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
030343-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.