Strain Name:
C57BL/6J-Cd3em1Btlr/Mmucd
Stock Number:
030345-UCD
Citation ID:
RRID:MMRRC_030345-UCD
Other Names:
tumormouse
Major Collection:

Strain Information

Cd3em1Btlr
Name: CD3 antigen, epsilon polypeptide; mutation 1, Bruce Beutler
Synonyms: tumormouse
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
Cd3e
Name: CD3 antigen, epsilon polypeptide
Synonyms: T3e, CD3, CD3epsilon
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12501
HGNC: HGNC:1674
Homologene: 586
Genetic Alterations
An ENU-induced C to A transition at position 330 of the Cd3e gene (Genbank Accession NM_007648) replaces the codon for tyrosine 84 with a premature stop codon. The early truncation results in a CD3epsilon null animal.
Genotype Determination
Phenotype
Homozygous: The tumormouse phenotype was identified in a screen for allogeneic sterility. The index mutant was injected intraperitoneally with L-929 fibroblasts of C3H background in order to pre-immunize against C3H alloreactive determinants. The animal developed an intra-abdominal tumor consisting of L-929 cells, hence the designation tumormouse.

Homozygous tumormouse animals have very small thymi with abnormal structure and no peripheral T cells. Tumormouse mutants have no CD4+CD8+ double positive (DP) T cells, and thymocyte development is arrested at the CD44+CD25- double negative (DN) 3 stage. Compared to wild type, tumormouse thymocytes display increased levels of apoptosis. B cells, macrophages and NK cells are found at normal levels in the periphery in tumormouse animals.

Some homozygous tumormouse animals older than 9 months of age appear to experience epileptic-like seizures.

Heterozygous: wild type
Mammalian Phenotype Terms
Allelic Composition: Cd3em1Btlr/Cd3em1Btlr (Genetic Background: C57BL/6J-Cd3em1Btlr )

Allelic Composition: Cd3em1Btlr/Cd3em1Btlr (Genetic Background: involves: C57BL/6J )

MeSH Terms
  • Animals
  • Apoptosis/immunology
  • Base Sequence
  • Cell Cycle Proteins/genetics
  • Cell Cycle Proteins/immunology
  • Cell Separation
  • Flow Cytometry
  • Immunologic Deficiency Syndromes/genetics
  • Immunologic Deficiency Syndromes/immunology
  • Leukocytes, Mononuclear/immunology
  • Lymphocyte Activation/immunology
  • Mice
  • Mice, Transgenic
  • Mutation
  • Phenotype
  • Signal Transduction/immunology
  • T-Lymphocytes/immunology
Strain Development
Original mutant is a C57BL/6J G3 ENU- induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Developmental Biology
  • Immunology and Inflammation
  • Research Tools
Donor
Bruce Beutler, M.D., The Scripps Research Institute
Primary Reference
  • Berger M; Krebs P; Crozat K; Li X; Croker BA; Siggs OM; Popkin D; Du X; Lawson BR; Theofilopoulos AN; Xia Y; Khovananth K; Y Moresco EM; Satoh T; Takeuchi O; Akira S; Beutler B, An Slfn2 mutation causes lymphoid and myeloid immunodeficiency due to loss of immune cell quiescence., Nat Immunol 2010 Apr;11(4):335-43 (Medline PMID: 20190759)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030345-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030345-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
030345-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.