Strain Detail Sheet

Strain Name:
C57BL/6J-Stat1m2Btlr/Mmucd
Stock Number:
030439-UCD
Citation ID:
RRID:MMRRC_030439-UCD
Other Names:
poison, C57BL/6J-Stat1m2Btlr/Mmcd
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Stat1m2Btlr
Name: signal transducer and activator of transcription 1; mutation 2, Bruce Beutler
Synonyms: poison
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
Stat1
Name: signal transducer and activator of transcription 1
Synonyms: 2010005J02Rik
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20846
Homologene: 21428
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Genetic Alterations
ENU-induced T to A transversion at position 31788 in the genomic DNA sequence of the Stat1 gene (Genbank genomic region NC_000067 for linear genomic DNA sequence of Stat1). The mutation is located within intron 19, thirteen nucleotides upstream from the start of exon 20, and impairs the acceptor splice site of intron 19. cDNA sequencing demonstrates the presence of several abnormal transcripts. In one case, a sequence in the middle of exon 20 (TCCTAG, position 31850-31855) is used as an acceptor splice site, causing a deletion of 55 nucleotides from the 5
Genotype Determination

Strain Description

Phenotype
Homozygous: The poison phenotype was identified among ENU-induced G3 mutant mice by screening for defects in natural killer (NK) cell function using an in vivo cytolytic NK cell assay. Briefly, mice were injected with fluorescently labeled splenocytes isolated from control C57BL/6J, allogeneic C3H/HeN, and TAP-deficent mice, and NK cell-specific killing was measured 48 hours later. Homozygous poison mice exhibit a reduced ability to kill TAP- deficient target cells lacking MHC class I molecules, but normal killing of C3H/HeN target cells displaying allogeneic MHC molecules. In vitro, NK cells from poison homozygotes produce normal levels of interferon (IFN)- gamma and degranulate normally after stimulation with antibodies against Ly49D, NK1.1, and NKp46 receptors, or after treatment with interleukin (IL)-12, IL-2 or IL-18. However, neither IFN-gamma production nor degranulation was observed when poison NK cells were exposed to YAC-1 tumor cells.

Poison homozygotes display increased susceptibility to infection by mouse cytomegalovirus (MCMV). 100% of infected mice succumb by day 5 post-infection with 105 pfu, a normally sublethal inoculum, making poison mice as susceptible to MCMV infection as BALB/c mice lacking the NK cell activating receptor Ly49H.

Heterozygous: wild type
Mammalian Phenotype Terms
Allelic Composition: Stat1m2Btlr/Stat1m2Btlr (Genetic Background: C57BL/6J-Stat1m2Btlr )
  • immune system
    • decreased NK cell degranulation [MP:0008791]
    • impaired natural killer cell mediated cytotoxicity [MP:0005070]
    • decreased interferon-gamma secretion [MP:0008567]
    • increased susceptibility to viral infection [MP:0002418]
  • hematopoietic system
    • decreased NK cell degranulation [MP:0008791]
    • impaired natural killer cell mediated cytotoxicity [MP:0005070]
Strain Development
Original mutant was a C57BL/6J G3 ENU- induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
  • Wildtype littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
  • Virology

Strain Origin

Donor
Bruce Beutler, M.D., The Scripps Research Institute
Primary Reference
Moresco EMY, Whitley K, Hoebe K, Beutler B. Record for poison, updated 29 April 2008. MUTAGENETIX (TM), B. Beutler and colleagues, Department of Genetics, The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Special Considerations


Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
030439-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
030439-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
030439-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.