Strain Name:
C57BL/6J-Tlr6m3Btlr/Mmucd
Stock Number:
030960-UCD
Citation ID:
RRID:MMRRC_030960-UCD
Other Names:
msd2, m2sd2, C57BL/6J-Tlr6m3Btlr/Mmcd
Major Collection:

Strain Information

Tlr6m3Btlr
Name: toll-like receptor 6; mutation 3, Bruce Beutler
Synonyms: MALP-2 specific defect 2, msd2, m2sd2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
Tlr6
Name: toll-like receptor 6
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 21899
Homologene: 21223
Genetic Alterations
The msd1 mutation results in an T to A transversion at position 1301 of the Tlr6 gene, and results in a conversion of an leucine to a stop codon at residue 390 and truncation of 416 amino acids from the C-terminus of the protein.
Genotype Determination
Phenotype
Homozygous: Peritoneal macrophages from msd2 mice display impaired tumor necrosis factor (TNF)-α lipopeptide-2, but showed normal TNF-α responses to the TLR2/1 ligand Pam3CSK4 (triacyl lipopeptide), the TLR4 ligand lipopolysaccharide (LPS), the TLR9 ligand CpG-DNA (single-stranded DNA), the TLR 7 ligand resiquimod/R-848 (single-stranded RNA mimetic), and the TLR3 ligand poly I:C (double-stranded RNA). Msd2 macrophages also responded normally to the TLR2/6 ligand peptidoglycan (PGN); contamination from a TLR1/2 ligand is likely as TLR6-deficient mice responded normally to this ligand, but TLR2-deficient animals did not.

Heterozygous: Same as wild-type.
Mammalian Phenotype Terms
Allelic Composition: Tlr6m3Btlr/Tlr6m3Btlr (Genetic Background: C57BL/6J-Tlr6m3Btlr )

  • immune system
  • hematopoietic system
Strain Development
The msd2 mutant was generated by ENU mutagenesis on a C57BL/6J background. This strain has been maintained on this background.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Bruce Beutler, M.D., The Scripps Research Institute
Primary Reference
Owen M. Siggs, Eva Marie Y. Moresco, Nora G. Smart, Bruce Beutler. Record for m2sd2, updated February 2, 2009. MUTAGENETIX (TM), B. Beutler and colleagues, Department of Genetics, The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

TLR6 signaling is abolished. Donor says the mice will probably be sensitive to certain infections but are healthy under normal conditions.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
030960-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.