Strain Detail Sheet

Strain Name:
B6;D2-Tg(CSN2-fat-1)1Vane/Mmucd
Stock Number:
031129-UCD
Citation ID:
RRID:MMRRC_031129-UCD
Other Names:
Fat 1-12

Strain Information

Gene Details

Csn2
Name: casein beta
Synonyms: CSN2, Csnb
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12991
HGNC: HGNC:2447
Homologene: 1426
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fat-1
Name: fatty acid desaturase
Type: Gene
Species: Caenorhabditis elegans (roundworm)
Chromosome: 4
Tg(CSN2-fat-1)1Vane
Name: transgene insertion 1, Alison Van Eenennaam
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The coding sequence of the C. elegans omega-3 fatty acid desaturase (fat-1) gene (GenBank accession number L41807) was cloned into the milk expression vector pBC1 (Invitrogen, Carlsbad, CA); transgenic mice express the Fat-1 gene under the control of the goat beta-casein promoter, which directs
transcription primarily to the mammary glands.
Genotype Determination
Genotyping Protocol

ES Cell Line
NA

Strain Description

Phenotype

Homozygous: High levels of omega-3 fatty acids in the milk produced by these mice as a result of expressing the Fat-1 gene in the mammary gland under the control of the goat beta-casein promoter

Hemizygous: High levels of omega-3 fatty acids in the milk produced by these mice as a result of expressing the Fat-1 gene in the mammary gland under the control of the goat beta-casein promoter

Mammals lack the n-3 fatty acid desaturase required for the synthesis of ?-linolenic acid and are therefore dependent on dietary sources to obtain this essential fatty acid, which among other roles is important for neuronal development. Pups nursed on transgenic dams had earlier eye opening and higher visual placement scores; there was no significant effect on other developmental parameters, such as weight gain, or novelty preference behavior. Thus, increasing the proportion of n-3 polyunsaturated fatty acid in the diet could have a beneficial effect in children on some aspects of cognitive
ability. Milk is a good food for augmentation because milk fat is dispersed in extremely small micelles, which improves the absorption and bioavailability of lipid-soluble compound.

MeSH Terms
  • Animals
  • Animals, Newborn
  • Brain Chemistry
  • Caenorhabditis elegans Proteins/genetics
  • Caenorhabditis elegans Proteins/metabolism
  • Caseins/genetics
  • Fatty Acid Desaturases/genetics
  • Fatty Acid Desaturases/metabolism
  • Fatty Acids/analysis
  • Fatty Acids, Omega-3/analysis
  • Fatty Acids, Omega-3/biosynthesis
  • Female
  • Gene Expression
  • Genetic Vectors
  • Goats/genetics
  • Mammary Glands, Animal/enzymology
  • Mice
  • Mice, Transgenic
  • Milk/chemistry
  • Milk/enzymology
  • Promoter Regions, Genetic/genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection
  • Animal Nutritional Physiological Phenomena
  • Animals, Suckling
  • Body Weight
  • Brain Chemistry/drug effects
  • Docosahexaenoic Acids/analysis
  • Fatty Acids/blood
  • Fatty Acids, Omega-3/blood
  • Fatty Acids, Omega-3/pharmacology
  • Fatty Acids, Unsaturated/blood
  • Fatty Acids, Unsaturated/pharmacology
  • Lactation/genetics
  • Male
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Models, Biological
  • Behavior, Animal
  • Brain/metabolism
  • Diet
  • Eicosapentaenoic Acid/metabolism
  • Exploratory Behavior
  • Eye/growth & development
  • Fatty Acids, Omega-6/administration & dosage
  • Fatty Acids, Omega-6/metabolism
  • Lactation/metabolism
  • Milk/metabolism
  • Visual Pathways/growth & development
  • Weight Gain
  • alpha-Linolenic Acid/metabolism
Strain Development
Pronuclear microinjection was performed by the Murine Targeted Genomics Laboratory of the UC Davis Mouse Biology Program.

  • Transgenic Fat-1 founder chimeric Fat1 toe 12 designated Fat1-12 was paired to C57 to produce the T1 heterozygous generation.
  • A T1 positive male was crossed to C57BL/6 to produce the T2 heterozygous generation
  • The T2 positive male was crossed to C57BL/6 to produce the T3 heterozygous generation
  • 2 positive T3 siblings were crossed to produce first homozygous male individual Fat1-12-TFS-20#3 (TFS indicates parents were Transgenic Full Siblings) (homozygosity proven by cross with C57BL/6 and progeny testing for all positive heterozygous offspring)
  • Homozygous male individual Fat1-12-TFS-20#3 was crossed to C57BL/6 to produce positive heterozygous offspring designated Fat1-12-hz-77.
  • Homozygous male individual Fat1-12-TFS-20#3 was crossed to his positive heterozygous offspring Fat1-12-hz-77 to produce homozygous individual siblings designated Fat1-12-50-1 and Fat1-12-50-4 (homozygosity proven by cross with C57BL/6 and progeny testing for all positive heterozygous offspring)
  • Homozygous siblings Fat1-12-50-1 and Fat1-12-50-4 were paired to produce positive homozygous litter Fat1-12-L-4
  • Fat1-12-L-4 siblings were paired to produce homozygous litter Fat1-12-L-8
  • Fat1-12- L-8 siblings were paired to produce homozygous litter Fat1-12-L-34
  • Fat1-12-L-34 siblings were paired to produce homozygous litter Fat1-12-L- 39
  • Fat1-12-L-39 siblings were paired to produce homozygous litter Fat1- 12-L-40
  • Fat1-12-L-39 had positive fat-1 gene by genotyping, had RNA expression of Fat-1 by RT-PCR using intron spanning fat-1 expression primers, and showed the altered fatty acid profile phenotype in their milk phospholipid fraction. 2 males and 1 female Fat1-12-L-39 mice are available, DOB 9/19/08
  • Fat1-12-L-39 siblings were paired to produce homozygous litter Fat1-12-L-40, that had positive fat-1 gene by genotyping.
  • The strain donor reports limited back crossing to C57BL/6 (N2).
    • Suggested Control Mice

    Wild-type littermates

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

    Research Applications

    • Diabetes/Obesity
    • neonatal brain development

    Strain Origin

    Donor
    Alison Van Eenennaam, Ph.D., University of California, Davis
    Primary Reference

    Kao BT, Lewis KA, DePeters EJ, Van Eenennaam AL. Endogenous production and elevated levels of long-chain n-3 fatty acids in the milk of transgenic mice. J Dairy Sci. 2006 Aug;89(8):3195-201. (Medline PMID: 16840637)

    Kao BT, DePeters EJ, Van Eenennaam AL. Mice raised on milk transgenically enriched with n-3 PUFA have increased brain docosahexaenoic acid. Lipids. 2006 Jun;41(6):543-9. (Medline PMID: 16981432)

    Bongiovanni KD, Depeters EJ, Van Eenennaam AL. Neonatal growth rate and development of mice raised on milk transgenically enriched with omega-3 fatty acids. Pediatr Res. 2007 Oct;62(4):412-6. (Medline PMID: 17667849)

    Colony and Husbandry Information

    Special Considerations

    Donor notes "some problems with breeding heterozygotes from this line -low numbers of pups born in a litter - varies".

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    031129-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    031129-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
    031129-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.