Strain Name:
C57BL/6J-Trfr2m1Btlr/Mmnc
Stock Number:
031132-UNC
Citation ID:
RRID:MMRRC_031132-UNC
Major Collection:

Strain Information

Tfr2m1Btlr
Name: transferrin receptor 2; mutation 1, Bruce Beutler
Synonyms: iron-man
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
Tfr2
Name: transferrin receptor 2
Synonyms: Trfr2, Tfr2
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 50765
Homologene: 2428
Genetic Alterations
The iron-man mutation results in a T to C transition in the donor splice site of intron 15 (position 13302 in the Genbank genomic region for linear genomic DNA sequence of Trfr2 on Chromosome 5).
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Homozygous: Homozygous iron- man mice display elevated levels of serum iron and ferritin, the main intracellular iron storage protein. Iron-man animals also display reduced levels of the hormone hepcidin, the major systemic iron regulator.

    Heterozygous: Homozygous iron- man mice display elevated levels of serum iron and ferritin, the main intracellular iron storage protein. Iron-man animals also display reduced levels of the hormone hepcidin, the major systemic iron regulator.
    Mammalian Phenotype Terms
    Allelic Composition: Tfr2m1Btlr/Tfr2m1Btlr (Genetic Background: C57BL/6J-Tfr2m1Btlr )

    • homeostasis/metabolism
    Strain Development

    Array

    A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

    MMRRC Strain Reference Strains Genomic makeup (Mb)
    Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
    C57BL/6J-Trfr2m1Btlr 31132 C57BL/6J 129S1/SvImJ 2582 0 0 0
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Hematology
    • Iron metabolism
    • Metabolism
    • Models for Human Disease
    Donor
    Bruce Beutler, M.D., The Scripps Research Institute
    Primary Reference
    Xin Du, Nora G. Smart, Bruce Beutler. Record for iron-man, updated May 26th, 2009. MUTAGENETIX (TM), B. Beutler and colleagues, Department of Genetics, The Scripps Research Institute, La Jolla, CA.

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    031132-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.