Strain Name:
B6.129X1-Runx2tm1Ldq/Mmmh
Stock Number:
032650-MU
Citation ID:
RRID:MMRRC_032650-MU

Strain Information

Runx2tm1Ldq
Name: runt related transcription factor 2; targeted mutation 1, L Darryl Quarles
Synonyms: Runx2-II-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knockout
Runx2
Name: runt related transcription factor 2
Synonyms: AML3, polyomavirus enhancer binding factor 2 (PEBP2), SL3-3 enhancer factor 1, PEBP2 alpha A, PEBP2aA, Osf2, Cbfa1, Pebpa2a
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Knockout
NCBI: 12393
Homologene: 68389
Genetic Alterations
Targeted deletion of Runx2-II isoform in Runx2 gene locus. The P1 promoter and Runx2-II exon 1 has been deleted in this strain.
Genotype Determination
ES Cell Line
Not specified
Phenotype
Homozygous: The homozygous Runx2-II knock-out mice are perinatal lethal, and have severe skeletal defects in both intramembraous and endochondral bone formation.

Heterozygous: The heterzygous phenotype in Runx2-II mice leads to low turn-over osteopenia in postnatal bone formation.
Mammalian Phenotype Terms
Allelic Composition: Runx2tm1Ldq/Runx2tm1Ldq (Genetic Background: involves: C57BL/6J )

Allelic Composition: Runx2tm1Ldq/Runx2+ (Genetic Background: involves: C57BL/6J )

MeSH Terms
  • Alkaline Phosphatase/metabolism
  • Animals
  • Bone and Bones/metabolism
  • Chondrocytes/metabolism
  • Core Binding Factor Alpha 1 Subunit
  • Exons
  • Genes, Reporter
  • Heterozygote
  • Homozygote
  • Mice
  • Mice, Mutant Strains
  • Models, Genetic
  • Neoplasm Proteins/genetics
  • Neoplasm Proteins/metabolism
  • Osteoblasts/metabolism
  • Phenotype
  • Promoter Regions, Genetic
  • Protein Isoforms
  • Protein Structure, Tertiary
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Tomography, X-Ray Computed
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Transcription, Genetic
  • Biomechanical Phenomena
  • Bone Density
  • Bone Diseases, Metabolic/genetics
  • Bone Diseases, Metabolic/metabolism
  • Bone Marrow Cells/metabolism
  • Bone Resorption/genetics
  • Bone Resorption/metabolism
  • Cells, Cultured
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Femur/diagnostic imaging
  • Femur/growth & development
  • Gene Expression Profiling
  • Genotype
  • Mice, Knockout
  • Mutation
  • Osteoclasts/metabolism
  • Osteogenesis
  • Protein Isoforms/deficiency
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Spine/diagnostic imaging
  • Spine/growth & development
  • Transcription Factor AP-2
  • Transcription Factors/deficiency
  • Body Weight
  • Bone Development/genetics
  • Bone and Bones/diagnostic imaging
  • Bone and Bones/embryology
  • Calcium/metabolism
  • Chromatin Immunoprecipitation
  • Core Binding Factor Alpha 1 Subunit/deficiency
  • Core Binding Factor Alpha 1 Subunit/genetics
  • Core Binding Factor Alpha 1 Subunit/metabolism
  • Embryo, Mammalian/metabolism
  • Gene Dosage/genetics
  • Gene Expression Regulation, Developmental
  • Homeostasis
  • Osteoblasts/enzymology
  • Osteocalcin/genetics
  • Osteocalcin/metabolism
  • Promoter Regions, Genetic/genetics
  • Protein Binding
  • X-Ray Microtomography
Strain Development
The original founder strain has been backcrossed with C57BL/6J for 12 generations, and then the current strain has been maintained for 6 sib-mated generations.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Research Tools
Donor
L. Darryl Quarles, M.D., University of Tennessee Health Science Center
Primary Reference
  • Xiao ZS, Hjelmeland AB, Quarles LD. 2004, Selective deficiency of the "bone-related" Runx2-II unexpectedly preserves osteoblast-mediated skeletogenesis. J Biol Chem 279(19):20307- 20313. (Medline PMID: 15007057)
  • Xiao ZS, Awad, H.A., Liu, S., Mahlios, J., Zhang, S., Guilak, F., Mayo, M.S., and Quarles, L.D. 2005, Selective Runx2-II deficiency leads to low-turnover osteopenia in adult mice. Dev Biol 283:345-356. (Medline PMID: 15936013)
  • Zhang SQ, Xiao ZS, Luo JM, He N, Mahlios J, Quarles L.D. 2009, Dose-dependent effects of Runx2 on bone development. J Bone Miner Res. Nov;24(11):1889-904. (Medline PMID: 19419310)

Colony and Husbandry Information

Sib-mating. Mice mated at ages between 6-24 weeks.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032650-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032650-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.