Strain Detail Sheet

Strain Name:
C57BL/6J-Tg(DCX-cre/ERT2)1Mull/Mmmh
Stock Number:
032780-MU
Citation ID:
RRID:MMRRC_032780-MU
Major Collection:
Neuroscience Blueprint

Strain Information

Gene Details

cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
cre/ERT2
Name: cre tamoxifen-dependent recombinase
Type: Gene
Species: Multi-species
Chromosome:
DCX
Name: doublecortin
Type: Gene
Species: Homo sapiens (human)
Chromosome: X
Tg(DCX-cre/ERT2)1Mull
Name: transgene insertion 1, Ulrich Mueller
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genotype Determination

Strain Description

Phenotype
Hemizygous DCX-CreERT2 mice are viable and fertile, with CreERT2 fusion gene expression directed by the human doublecortin (DCX) promoter/enhancer regions. The donating investigator reports that no abnormal phenotype is observed in hemizygous mice. Cre-ERT2 fusion gene activity is inducible; observed following taxomifen administration. As such, when DCX-CreERT2 mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the DCX-expressing cells of the offspring. The donating investigator reports tamoxifen-inducible Cre recombinase activity is observed throughout the central nervous system, including most brain regions and spinal cord. The donating investigator did not examine Cre-ERT2 activity in tissues other than brain, or in any tissues prior to tamoxifen treatment.
Strain Development

A transgene was designed with the human doublecortin (DCX) promoter/enhancer regions upstream of a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain). This transgene was microinjected into C57BL/6J blastocysts. Transgenic mice from a single founder line were bred to C57BL/6J mice. These DCX-CreERT2 transgenic mice were subsequently maintained by breeding hemizygous mice with C57BL/6J inbred mice for many generations prior to arrival at the MMRRC.

A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC Strain Reference Strains Genomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
C57BL/6J-Tg(DCX-cre/ERT2)1Mull/Mmmh 32780 C57BL/6J 129S1/SvImJ 2584 0 0 0
Suggested Control Mice
  • Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Strain Origin

Donor
Ulrich Mueller, Ph.D., The Scripps Research Institute
Primary Reference
Additional information regarding this strain can be found at NIH Neuroscience Blueprint Cre Driver Network.

Colony and Husbandry Information

Special Considerations

When maintained as a live colony, transgene carrier mice may be bred together, to noncarrier (wildtype) mice from the colony, or to C57BL/6J inbred mice.

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@missouri.edu

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.