Strain Detail Sheet

Strain Name:
B6;FVB-Tg(CMV-ENPP1)1Brem/Mmucd
Stock Number:
032878-UCD
Citation ID:
RRID:MMRRC_032878-UCD
Other Names:
PC-1, B6;FVB-Tg(CMV-ENPP1 )1Brem/Mmcd

Strain Information

Gene Details

Enpp1
Name: ectonucleotide pyrophosphatase/phosphodiesterase 1
Synonyms: PC-1, NPP1, Ly-41, Npps, Pca-1, Pca, Pdnp1, twy, CD203c, E-NPP1, 4833416E15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18605
HGNC: HGNC:3356
Homologene: 38151
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
CMV
Name: cytomegalovirus
Type: Regulatory Region
Species: Virus
Chromosome:
Tg(CMV-ENPP1)1Brem
Name: transgene insertion 1, Andrew Bremer
Synonyms: PC-1
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The wild-type full-length human PC-1 q allele was subcloned into plasmid pRK7 under the control of the CMV promoter, as previously described (11). Transgenic animals were made as follows. Gene constructs were freed of plasmid sequences by gel electrophoresis after appropriate restriction enzyme digestion. The DNA was injected into the pronucleus of fertilized mouse eggs harvested from recently mated and superovulated, 4- to 6-wk-old female, white FVN mice and black B6D2 mice. After the animals were euthanized, the recovered eggs were allowed to recover, and the DNA was then injected into pronuclei. The injected eggs were then transferred to the oviducts of pseudo-pregnant females. At 3
Genotype Determination

Genotyping Protocol

Strain Description

Phenotype
Homozygous: Homozygous PC-1+/+ mice have improved glucose tolerance and insulin sensitivity compared to wild type.

Heterozygous/Hemizygous: Heterozygous PC-1+/+ mice have improved glucose tolerance and insulin sensitivity compared to WT, but diminished glucose tolerance and insulin sensitivity compared to homozygous PC-1+/+.
Strain Development
Originally the human PC-1 transgene to Q allele was introduced into FVB/N stain because it is easier to obtain transgenic mice from this strain. We obtained 6 founders, with 1 line having high PC-1 expression in both liver and muscle. The F1 generation was bred with C57B6 to produce a F1 hybrid (F1H). The PC-1 transgenic hybrids were bred with C57B6 until F3H generation and then were inbred for 4 more generations.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Diabetes/Obesity
  • Metabolism
  • Models for Human Disease

Strain Origin

Donor
Andrew A. Bremer, M.D., Ph.D., University of California, Davis

Colony and Husbandry Information

Special Considerations

Tg male or female backcrossed to B6 male or female. All breeding pairs and their pre-wean litters are housed in shoebox cages with water bottles and receive cotton nestlet. They receive a standard normal lab rodent chow. All non-breeding mice are housed in shoebox cages with water bottles and nestlet; they also receive a standard normal lab rodent chow.

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
032878-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit		 Aliquot Approximate quantity2 : 20-40 embryos / aliquot
032878-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit		 Aliquot Approximate quantity3
032878-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.