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Homozygous: Not evaluated
Heterozygous: These Ple176-EGFPCre;mEMS759 mice have the Ple176-EGFPCre transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene directing expression of an enhanced green fluorescent protein/Cre recombinase fusion protein (EGFPCre). These Ple176-EGFPCre;mEMS759 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.
The donating investigator reports: When these Ple176-EGFPCre;mEMS759 mice are bred to also harbor the Gt(ROSA)26Sortm1Sor mutant allele, strong Cre recombinase activity (βGal staining) is detected widely throughout the brain and eye, with notable expression in the hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum and retina. In each case, the staining appears mosaic, thus not all cells are βGal-positive, but a significant subpopulation of cells across many regions are labeled. This mosaicism is emphasized in the labeling of radial columns of cells in the cortex and the retina. A majority of the βGal-positive cells of the grey matter appear to be neuronal, as they co-label with the neuronal marker NeuN. However, it is apparent that the reporter is not restricted to neurons, but is also detected in cells within the white matter (notably in the corpus callosum, hippocampal fimbria, and the cerebellar white matter), suggestive of glial labeling as well. EGFP expression was not detected in any cells of the adult brain, indicating βGal expression is an historical marker of expression of the EGFP/cre reporter. The pattern of Cre recombinase activity (βGal expression) is very similar to that seen for the Ple177 and the Ple178 strains.
Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric mice (founder line mEMS759) were bred to B6.129S4-Gt(ROSA)26Sortm1Sor/J mice to establish the mutant colony. These Ple176-EGFPCre;mEMS759 mutant mice were bred with B6.129S4-Gt(ROSA)26Sortm1Sor/J mice for multiple generations. After this, hemizygous sperm was sent to the MMRRC. Upon arrival, Ple176-EGFPCre;mEMS759 mutant mice were backcrossed to C57BL/6J inbred mice (selecting away from the Gt(ROSA)26Sortm1Sor mutation) to establish this congenic strain.
A 27 SNP (single nucleotide polymorphism) analysis performed by the MMRRC revealed 6 of 27 markers that were not C57BL/6 allele-type. Therefore this strain is not congenic on a C57BL/6 genetic background.
Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.