Strain Name:
B6.Cg-Tg(tetO-tTA,-Rag1,-Rag2)1Dgs/Mmmh
Stock Number:
033007-MU
Citation ID:
RRID:MMRRC_033007-MU

Strain Information

tTA
Name: tetracycline-controlled transactivator protein
Type: Gene
Species: Multi-species
Chromosome:
tetOp
Name: tetracycline operon
Type: Gene
Species: Synthetic
Chromosome:
Rag1
Name: recombination activating 1
Synonyms: Rag-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19373
HGNC: HGNC:9831
Homologene: 387
Rag2
Name: recombination activating gene 2
Synonyms: Rag-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19374
HGNC: HGNC:9832
Homologene: 7507
Tg(tetO-tTA,-Rag1,-Rag2)1Dgs
Name: transgene insertion 1, David G Schatz
Synonyms: Tg(tetO-tTA, tetO-Rag1, tetO-Rag2)1Dgs
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Three transgenes are cointegrated and inherited en block. The transgenes contain the tTA (tet transactivator), RAG-1, and RAG-2 genes each driven by the autoregulatory tet system promoter, ie. the minimal CMV promoter and 7 copies of the tet operator. Mice widely and inducibly express the tTA and Rag transgenes when they are not exposed to tetracycline in their food or water. The transgenes are suppressed in the presence of tetracycline.
Genotype Determination
Phenotype
Homozygous: The transgenes reconstitute lymphocyte development on the RAG 1 or RAG 2 knockout background. They also potentially allow regulated expression of the transgenes in some nonlymphoid tissues.

Hemizygous: Same as homozygous.
MeSH Terms
  • 3T3 Cells
  • Animals
  • Blotting, Western
  • Cells, Cultured
  • DNA Nucleotidyltransferases/biosynthesis
  • DNA Nucleotidyltransferases/metabolism
  • Gene Expression Regulation/drug effects
  • Herpes Simplex Virus Protein Vmw65/biosynthesis
  • Mice
  • Mice, Transgenic
  • Open Reading Frames
  • Plasmids
  • RNA, Messenger/analysis
  • RNA, Messenger/biosynthesis
  • Recombinant Fusion Proteins/biosynthesis
  • Repressor Proteins/biosynthesis
  • Restriction Mapping
  • Sequence Deletion
  • Tetracycline/pharmacology
  • Trans-Activators/biosynthesis
  • Transfection
  • VDJ Recombinases
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Flow Cytometry
  • Gene Rearrangement
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Immunoglobulins/genetics
  • Immunoglobulins/immunology
  • Lymphoid Tissue/immunology
  • Lymphoid Tissue/physiology
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Receptors, Antigen, T-Cell/genetics
  • T-Lymphocytes/immunology
  • T-Lymphocytes/physiology
  • Tetracycline/metabolism
  • Gene Expression Regulation/genetics
  • Gene Targeting/methods
  • Genetic Markers/drug effects
  • Genetic Markers/genetics
  • Genetic Vectors/genetics
  • Mice, Transgenic/genetics
  • Molecular Biology/methods
  • NIH 3T3 Cells
  • Plasmids/genetics
  • Trans-Activators/genetics
  • Transfection/methods
Strain Development
These mice were bred onto the B6 background (>10 generations) and then maintained as homozygotes for several generations (>4 years) at Yale Medical School. In 2004 they came to Southeastern Louisiana University and have been maintained as heterozygotes for approximately 14 backrosses to C57Bl/6.
Suggested Control Mice

Wildtype littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cancer
  • Developmental Biology
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
Donor
Penny Shockett Ph.D., Southeastern Louisiana University
Primary Reference
  • Shockett P, Difilippantonio M, Hellman N, Schatz DG.A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice. Proc Natl Acad Sci U S A. 1995 Jul 3;92(14): 6522-6. (Medline PMID: 7604026)
  • Shockett PE, Zhou S, Hong X, Schatz DG. Partial reconstitution of V(D)J rearrangement and lymphocyte development in RAG-deficient mice expressing inducible, tetracycline-regulated RAG transgenes. Mol Immunol. 2004 Jan;40(11):813-29. (Medline PMID: 14687938)
  • Shockett P, Schatz D. Inducible gene expression using an autoregulatory, tetracycline-controlled system. Curr Protoc Cell Biol. 2005 Jul;Chapter 20: Unit 20.8. (Medline PMID: 18228465)

Colony and Husbandry Information

Backcrossed to B6.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
033007-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
033007-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.