Strain Name:
C57BL/6J-Irf8m1Btlr/Mmucd
Stock Number:
034294-UCD
Citation ID:
RRID:MMRRC_034294-UCD
Other Names:
Gemini, C57BL/6J-Irf8m1Btlr/Mmcd
Major Collection:

Strain Information

Irf8M1Btlr
Name: interferon regulatory factor 8; mutation 1, Bruce Beutler
Synonyms: Gemini
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
Irf8
Name: interferon regulatory factor 8
Synonyms: IRF-8, Myls, ICSBP, Icsbp1
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 15900
HGNC: HGNC:5358
Homologene: 1629
Genetic Alterations
The Gemini mutation corresponds to a C to T transition at position 387 of the Irf8 transcript in exon 3 of 9 total exons using Genbank record NM_008320. This converts a glutamine at amino acid 118 to a stop codon removing 307 amino acids from the C-terminus of the encoded protein.
Genotype Determination
Phenotype
Homozygous: In the spleen, there is a reduction in the frequency of mature follicular B cells and an expansion of the marginal zone B cell compartment. Complete absence of monocytes and mature macrophages in the blood, peritoneal cavity, and bone marrow. There is also expansion of granulocytes, most likely neutrophils, in the blood, spleen, and bone marrow. The mice are unable to make type I interferon (IFN) after CpG DNA challenge in vivo. Animals are susceptible to non-lethal doses of MCMV.

Heterozygous: Heterozygous animals may have an intermediate phenotype but this has not been confirmed.
Mammalian Phenotype Terms
Allelic Composition: Irf8M1Btlr/Irf8M1Btlr (Genetic Background: C57BL/6J-Irf8M1Btlr )

MeSH Terms
  • Adaptor Protein Complex 3/metabolism
  • Animals
  • Carrier Proteins/metabolism
  • Cell Membrane/metabolism
  • Chromosome Mapping
  • Dendritic Cells/cytology
  • Dendritic Cells/immunology
  • Genetic Testing
  • Hermanski-Pudlak Syndrome/metabolism
  • Interferon Type I/biosynthesis
  • Lectins/metabolism
  • Membrane Transport Proteins/metabolism
  • Mice
  • Mutation/genetics
  • Nerve Tissue Proteins/metabolism
  • Protein Transport
  • Signal Transduction/immunology
  • Toll-Like Receptors/metabolism
  • Vesicular Transport Proteins
Strain Development
The Gemini strain was generated by ENU on a C57BL/6J background and was maintained on this background.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunology and Inflammation
Donor
Bruce Beutler, M.D. The Scripps Research Institute.
Primary Reference
  • Blasius AL, Arnold CN, Georgel P, Rutschmann S, Xia Y, Lin P, Ross C, Li X, Smart NG, Beutler B. Slc15a4, AP-3, and Hermansky-Pudlak syndrome proteins are required for Toll-like receptor signaling in plasmacytoid dendritic cells, Proc Natl Acad Sci U S A. 2010;107(46):19973-8 (Medline PMID: 21045126)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute, La Jolla, CA.

Colony and Husbandry Information

Random intra-strain mating.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034294-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.