Strain Name:
B6;129-Vegfatm4Pec/Mmjax
Stock Number:
034406-JAX
Citation ID:
RRID:MMRRC_034406-JAX

Strain Information

Vegfatm4Pec
Name: vascular endothelial growth factor A; targeted mutation 4, Peter Carmeliet
Synonyms: VEGF188
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knock-In
Vegfa
Name: vascular endothelial growth factor A
Synonyms: VEGF164, VEGF-A, Vegf, VEGF120, VEGF188, VPF
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Knock-In
NCBI: 22339
Homologene: 2534
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Mice homozygous for the targeted mutation are viable, but exhibit reduced fertility, smaller litter sizes and some prenatal lethality. Surviving homozygotes gain less weight than wild-type controls. Retinal arterial development in VEGF188 mice is impaired. By post natal day 5 (P5), only half the normal number of large vessels (mostly venules) are present in the vascular bed of the retina. By P9, arterioles are observed to be smaller in size and only 50% have developed and grown out over 17% of the retina. In controls, 88% of the retinal radius is covered by P9. Venous and capillary development appears normal. VEGF188 mice express a VEGF protein with all eight exons that avidly binds to the cell surface and extracellular matrix. This mutant mouse strain may be useful in studies of retinal angiogenesis.

Heterozygous phenotype: Heterozygote phenotype is similar to the wild-type phenotype.
Mammalian Phenotype Terms
Allelic Composition: Vegfatm4Pec/Vegfatm4Pec (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

MeSH Terms
  • Animals
  • Arterioles/abnormalities
  • Arterioles/growth & development
  • Base Sequence
  • Body Patterning/genetics
  • Body Patterning/physiology
  • DNA/genetics
  • Endothelial Growth Factors/genetics
  • Endothelial Growth Factors/physiology
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Targeting
  • Lymphokines/genetics
  • Lymphokines/physiology
  • Male
  • Mice
  • Mice, Transgenic
  • Pregnancy
  • Protein Isoforms/genetics
  • Protein Isoforms/physiology
  • Receptor Protein-Tyrosine Kinases/genetics
  • Receptors, Growth Factor/genetics
  • Receptors, Vascular Endothelial Growth Factor
  • Retinal Vessels/abnormalities
  • Retinal Vessels/growth & development
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • Venules/abnormalities
  • Venules/growth & development
Strain Development
A targeting vector was designed to insert a loxP-flanked neomycin cassette into the 3' UTR of a cDNA containing fused exons 4 through 8. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1 derived R1 embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were bred to C57BL/6 and then maintained by sibling mating. The VEGF188 isoform contains all 8 exons. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
Donor
Patricia D'Amore, Ph.D., Schepens Eye Research Institute.
Primary Reference
Stalmans I; Ng YS; Rohan R; Fruttiger M; Bouche A; Yuce A; Fujisawa H; Hermans B; Shani M; Jansen S; Hicklin D; Anderson DJ; Gardiner T; Hammes HP; Moons L; Dewerchin M; Collen D; Carmeliet P; D'Amore PA, Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms., J Clin Invest. 2002 Feb;109(3):327-36 (Medline PMID: 11827992)

Colony and Husbandry Information

When maintaining a live colony, these mice can be bred as heterozygotes or homozygotes, however, homozygotes occur at a lower than Mendelian ratio (12%).

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
034406-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
034406-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.