Strain Name:
B6N.Cg-Tg(tetOp-Polb/tTA)2Sbl/Mmmh
Stock Number:
000356-MU
Citation ID:
RRID:MMRRC_000356-MU

Strain Information

tTA
Name: tetracycline-controlled transactivator protein
Type: Gene
Species: Multi-species
Chromosome:
Polb
Name: polymerase (DNA directed), beta
Synonyms: A430088C08Rik, Pol beta
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18970
HGNC: HGNC:9174
Homologene: 2013
tetO
Name: tetracycline-responsive promoter element
Type: Regulatory Region
Species: Multi-species
Chromosome:
Tg(TetOp-Polb/tTA)2Sbl
Name: transgene insertion 2, Robert W Sobol
Synonyms: TetOp-Flag_polbeta-tTA
Type: Transgene
Species: Multi-species
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
Random transgenic insertion of the tetO promoter driving expression of a Flag-tagged beta-polymerase minigene (Polb) and the tetracycline transactivator (tTA) from a bicistronic mRNA via the EMC virus IRES sequence.
Genotype Determination
Phenotype
Over-expression of Flag-tagged beta-polymerase (Flag-beta-pol) in the lens epithelium results in the early onset of severe cortical cataract, with cataractogenesis beginning within 4 days after birth. In utero and post-natal suppression of transgenic Flag-beta-pol expression by doxycycline administration completely prevents cataract formation through adulthood, yet cataract is subsequently observed following removal of doxycycline and re-expression of the transgene. Cataract development accompanies increased expression of cyclooxygenase-2 in the lenticular fibers of the lens, implicating oxidative stress in the development of this cataractous phenotype. The phenotype is expressed in both hemizygous and homozygous mice. Eye color is white due to cataracts.
MeSH Terms
  • Animals
  • Anti-Bacterial Agents/pharmacology
  • Base Pair Mismatch
  • Cataract/metabolism
  • Cell Differentiation
  • Cyclooxygenase 2
  • DNA Polymerase beta/biosynthesis
  • DNA Polymerase beta/genetics
  • DNA Repair
  • Doxycycline/pharmacology
  • Epithelial Cells/cytology
  • Escherichia coli/metabolism
  • Immunohistochemistry
  • Isoenzymes/biosynthesis
  • Mice
  • Mice, Transgenic
  • Models, Biological
  • Models, Molecular
  • Oxidative Stress
  • Phenotype
  • Prostaglandin-Endoperoxide Synthases/biosynthesis
  • Time Factors
  • Transgenes
Strain Development
The Flag-beta-pol over-expression transgenic mouse model was developed by directing bicistronic expression of a beta-pol minigene and the tetracycline transactivator (tTA) via the tetracycline promoter tetO (formerly known as "TetOp"). Transgenic B6SJLF1 mice expressing Flag-epitope tagged beta-pol were developed using standard pronuclear injection techniques. Genotyping of the transgenic mice was conducted by PCR. PCR amplification was carried out on genomic DNA isolated from tail tips.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Models for Human Disease
  • Research Tools
Donor
Robert W. Sobol, Ph.D., Hillman Cancer Center
Primary Reference
Sobol, R. W., Foley, J. F., Nyska, A., Davidson, M. G. & Wilson, S. H. Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice. DNA Repair 2, 609-622 (2003). (Medline PMID: 12713817)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
White
MMRRC Breeding System
Backcross
Breeding Scheme(s)
C57BL/6NTac female x Hemizygous male
Generation
N5+ (C57BL/6NTac)
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: unknown unknown
Average litter size
7 pups
Recommended wean age
4 weeks
Average Pups Weaned
7 pups

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000356-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000356-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
000356-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.