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Homozygous: Cellophane mice are unable to mount T-independent IgM responses following immunization with (4-hydroxy-3-nitrophenylacetyl)-Ficoll (NP-Ficoll). Most mice make suboptimal T-dependent IgG responses to a recombinant Semliki Forest virus (rSFV)-encoded β-galactosidase (rSFV-βGal). The cellophane mutation causes a slight reduction in peripheral T cell frequencies, but has no major effect on the frequency of mature B cells, although B cells from cellophane mice express lower surface levels of IgD than B cells from wild-type mice. The cellophane mice had reduced amounts of MZ B cells and peritoneal B1 B cells. Cellophane mice had significant changes in thymic cell subsets (i.e. DN and SP thymocytes were increased; DP thymocytes were decreased). Splenic NK cells were reduced. The frequency of GC B cells is reduced in the cellophane mice. Cellophane mice had normal levels of all Ig subtypes assayed with the exception of IgG2a, which was slightly reduced. To test for extrafollicular antibody responses, the cellophane mice were injected with NP-CGG. Seven days post-immunization the mice were assayed and found to have undetectable levels of NP-specific IgM and low levels of IgG1. At day 14 post-immunization, the cellophane IgG1 response was reduced approximately two-fold compared to wild-type animals. The IgG1 in the cellophane mice was determined to be low affinity, indicating that these mice do not support functional GCs. Splenic B cells from the cellophane mice cannot transfer NP-specific memory to naïve cells. Zeb1 is induced quickly after B-cell receptor cross-linking. Splenic B cells did not proliferate in response to B-cell receptor cross-linking when the amount of F(ab')2αIgM is limiting. Cellophane B cells were able to proliferate after stimulation with CpG; proliferation in response to LPS was impaired.
Heterozygous: Not tested
Arnold CN, Pirie E, Dosenovic P, McInerney GM, Xia Y, Wang N, Li X, Siggs OM, Karlsson Hedestam GB, Beutler B. A forward genetic screen reveals roles for Nfkbid, Zeb1, and Ruvbl2 in humoral immunity. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12286-93. Epub 2012 Jul 3. (Medline PMID: 22761313).
Arnold CN, Pirie E, Moresco EY, Murray A, Beutler B. Record for cellophane. MUTAGENETIXtm, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Colony Surveillance Program and Current Health Reports
Heterozygous female x Heterozygous male
Homozygous female x Homozygous male
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.