Strain Name:
C57BL/6J-Rab38m1Btlr/Mmmh
Stock Number:
036964-MU
Citation ID:
RRID:MMRRC_036964-MU
Other Names:
fenrir
Major Collection:

Strain Information

Rab38m1Btlr
Name: RAB38, member RAS oncogene family; mutation 1, Bruce Beutler
Synonyms: fenrir
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
Rab38
Name: RAB38, member RAS oncogene family
Synonyms: 2310011F14Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72433
HGNC: HGNC:9776
Homologene: 21353
Genetic Alterations
Whole exome HiSeq sequencing of the G1 grandsire identified 61 mutations. Five G3 mice with the fenrir phenotype (and 23 unaffected mice) were genotyped at all 61 mutation sites and four mutations on chromosome 7 were homozygous in all five of the fenrir mice. The mutation in Rab38 (LOD = 3.29) was presumed to be causative because the fenrir hypopigmentation phenotype mimics other known alleles of Rab38 (see MGI for a list of Rab38 alleles). Capillary sequencing of the mutated genes identified a T to A transversion at base pair 88450541 (v38) on Chromosome 7 in the GenBank genomic region NC_000073 encoding Rab38. The mutation corresponds to residue 263 in the mRNA sequence within exon 2 of 3 total exons. The mutation results in a Isoleucine (I) to Asparagine (N) at position 88 (I88N). This model was generated at UT Southwestern Medical Center at Dallas.
Genotype Determination
Phenotype

Homozygous: The fenrir mouse was visually identified among G3 mice homozygous for mutations induced by N-ethyl-N-nitrosourea (ENU) as a hypopigmentation mutant with brown fur.

Heterozygous: Not tested

Mammalian Phenotype Terms
Allelic Composition: Rab38m1Btlr/Rab38m1Btlr (Genetic Background: involves: C57BL/6J )

Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Hematology
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference

Purrington T, Murray A, Beutler B. Record for fenrir. MUTAGENETIXtm, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Brown
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)

Homozygous female x Homozygous male from the colony

Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Unknown Unknown
Heterozygotes are fertile: Unknown Unknown
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Not specified
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036964-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036964-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.