Strain Name:
STOCK Tg(CAG-Hsp70-1)1Whd/Mmucd
Stock Number:
037569-UCD
Citation ID:
RRID:MMRRC_037569-UCD
Other Names:
hsp70iTg

Strain Information

CAG
Name: CMV early enhancer/chicken beta actin promoter/rabbit beta globin splice acceptor
Type: DNA Segment
Species: Multi-species
Chromosome:
Tg(CAG-Hsp70-1)1Whd
Name: transgene insertion 1, Wolfgang H Dillmann
Synonyms: Tg(CAG-Hsp70-1)1Whd, HSP72Tg, Tg(Actb-Hsp70-1)1Whd
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The rat Hsp70-1 gene under the control of human CMV immediate early enhancer and chicken beta-actin promoter sequence (including intron 1) was used as the transgene. Overexpression of the rat transgene was verified by Western blot analysis of heart and Northern blot analysis of cardiac and skeletal tissue from transgenic animals.
Genotype Determination
Phenotype

Homozygous: Not characerized

Hemizygous: Overexpression of the rat transgene Hxp70 driven by a CAG promoter

MeSH Terms
  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Creatine Kinase/analysis
  • HSP70 Heat-Shock Proteins/biosynthesis
  • HSP70 Heat-Shock Proteins/genetics
  • HSP70 Heat-Shock Proteins/immunology
  • Hemodynamics
  • Immunity, Innate
  • In Vitro Techniques
  • Mice
  • Mice, Transgenic
  • Myocardial Contraction
  • Myocardial Infarction/pathology
  • Myocardial Ischemia/pathology
  • RNA, Messenger/biosynthesis
  • Rats
  • Recombinant Proteins/biosynthesis
  • Reperfusion Injury/prevention & control
  • Risk
  • Aged
  • Aged, 80 and over
  • Biomarkers/blood
  • Biomarkers/metabolism
  • Case-Control Studies
  • Disease Models, Animal
  • Female
  • HSP27 Heat-Shock Proteins/blood
  • HSP70 Heat-Shock Proteins/blood
  • HSP70 Heat-Shock Proteins/metabolism
  • HSP90 Heat-Shock Proteins/blood
  • Heart Failure/diagnosis
  • Heart Failure/metabolism
  • Heart Failure/physiopathology
  • Humans
  • Male
  • Middle Aged
  • Natriuretic Peptide, Brain/blood
  • Natriuretic Peptide, Brain/metabolism
  • Peptide Fragments/blood
  • Peptide Fragments/metabolism
  • ROC Curve
  • Risk Factors
  • Severity of Illness Index
Strain Development
The chimeric transgene was injected into (C57BL/6J x SJL/J)F1 fertilized eggs. The transgenic founders were bred again to (C57BL/6J x SJL/J)F1. Subsequently, the mice were bred to (BALB/c x C57BL/6)F1 mice and maintained hemizygous.
Mice will be recovered with CB6F1/Crl, (BALB/cAnN x C57BL/N)F1Crl hybrid donors.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Immunology and Inflammation
  • Neurobiology
Donor
Wolfgang H. Dillmann, M.D., University of California, San Diego.
Primary Reference
  • Marber MS, Mestril R, Chi SH, Sayen MR, Yellon DM, Dillmann WH. Overexpression of the rat inducible 70-kD heat stress protein in a transgenic mouse increases the resistance of the heart to ischemic injury. J Clin Invest. 1995 Apr;95(4):1446-56. (Medline PMID: 7706448)
  • Li Z, Song Y, Xing R, Yu H, Zhang Y, Li Z, Gao W. Heat shock protein 70 acts as a potential biomarker for early diagnosis of heart failure. PLoS One. 2013 Jul 9;8(7):e67964. (Medline PMID: 23874478)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black, brown or agouti
MMRRC Breeding System
Outcross to non-inbred (hybrid or outbred) mate
Breeding Scheme(s)
  • Wild-type female x Hemizygous male 
  • Homozygous female x Homozygous male
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: >12 months >12 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
037569-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
037569-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
037569-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.