Strain Detail Sheet

Strain Name:
C57BL/6J-Tnfsf13bM1Btlr/Mmmh
Stock Number:
038182--TBD-
Citation ID:
RRID:MMRRC_038182--TBD-
Other Names:
Frozen
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Tnfsf13bM1Btlr
Name: tumor necrosis factor (ligand) superfamily, member 13b; mutation 1, Bruce Beutler
Synonyms: Frozen
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
Tnfsf13b
Name: tumor necrosis factor (ligand) superfamily, member 13b
Synonyms: BAFF, zTNF4, BLyS, TALL-1, D8Ertd387e
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 24099
Homologene: 48443
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Genetic Alterations
ENU-induced T to A transversion at base pair 10,031,661 (v38) on chromosome 8, or base pair 25,469 in the GenBank genomic region NC_000074 for the Tnfsf13b gene within the donor splice site of intron 6. The effect of the mutation at the cDNA and protein level have not examined, but the mutation is predicted to result in skipping of the 151-nucleotide exon 6 (out of 7 total exons), a frame-shift that results in coding of 34 aberrant amino acids followed by a premature stop codon.

Strain Description

Phenotype

Homozygous: Mice exhibit reduced frequency of B cells, a reduced frequency of IgM+ B cells, a decreased percentage of IgD+ B cells, all in the peripheral blood. Increased frequency of T cells and an increased frequency of CD8+ T cells, all in the peripheral blood. Diminished T-dependent antibody response to recombinant Semliki Forest virus (rSFV)-β-galactosidase (rSFV-β-gal)]. The T-independent antibody response to 4-hydroxy-3-nitrophenylacetyl-Ficoll (NP-Ficoll) was also reduced.

Heterozygous: Mice exhibit reduced frequency of B cells, a reduced frequency of IgM+ B cells, a decreased percentage of IgD+ B cells, all in the peripheral blood. Increased frequency of T cells and an increased frequency of CD8+ T cells, all in the peripheral blood. Diminished T-dependent antibody response to recombinant Semliki Forest virus (rSFV)-β-galactosidase (rSFV-β-gal). The T-independent antibody response to 4-hydroxy-3-nitrophenylacetyl-Ficoll (NP-Ficoll) was also reduced. All phenotypes are intermediate between that in wild-type and homozygous Frozen mice.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Primary Reference

Choi JH, Wang K-W, Zeng M, Murray AR, Beutler B. Record for Frozen. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

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