Strain Detail Sheet

Strain Name:
C57BL/6J-Ciitam1Btlr/Mmmh
Stock Number:
038195--TBD-
Citation ID:
RRID:MMRRC_038195--TBD-
Other Names:
sisal
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Ciitam1Btlr
Name: class II transactivator; mutation 1, Bruce Beutler
Synonyms: sisal
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
Ciita
Name: class II transactivator
Synonyms: Gm9475, C2ta
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 12265
VEGA: 16
HGNC: HGNC:7067
Homologene: 207
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Genetic Alterations
ENU-induced G to A transition at base pair 10,513,288 (v38) on chromosome 16, or base pair 33,274 in the GenBank genomic region NC_000082 for the Ciita gene, within the donor splice site of intron 11. The effect of the mutation at the cDNA and protein level has not examined, but the mutation is predicted to result in an in-frame skipping of the 159-nucleotide exon 11 (out of 19 total exons).

Strain Description

Phenotype

Homozygous: Mice showed reduced frequencies of CD4+ T cells including na ve CD4+ T cells in CD4+ T cells and CD4+ T cells in CD3+ T cells as well as increased CD44+ CD4+ mean fluorescence intensity, all in the peripheral blood. Homozygotes also exhibited increased frequencies CD8+ T cells in CD3+ T cells in the peripheral blood. Some mice also exhibited diminished T-dependent antibody responses to ovalbumin administered with aluminum hydroxide and recombinant Semliki Forest virus (rSFV)-β-galactosidase (rSFV-β-gal).

Heterozygous: No abnormal phenotype detected.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
    • abnormal CD4-positive T cell differentiation [MP:0008076]
    • abnormal CD8-positive, alpha-beta T cell differentiation [MP:0008080]
    • impaired humoral immune response [MP:0020154]
  • hematopoietic system
    • abnormal CD4-positive T cell differentiation [MP:0008076]
    • abnormal CD8-positive, alpha-beta T cell differentiation [MP:0008080]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation
  • Models for Human Disease
    • Complementation group A bare lymphocyte syndrome (BLS), type II [OMIM: #209920]]

Strain Origin

Primary Reference

Wang K-W, Choi JH, Watthanasurorot A, Murray AR, Beutler B. Record for sisal. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

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