Strain Detail Sheet

Strain Name:
C57BL/6J-Nlrc4m1Btlr/Mmmh
Stock Number:
038229--TBD-
Citation ID:
RRID:MMRRC_038229--TBD-
Other Names:
inwood
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Nlrc4m1Btlr
Name: NLR family, CARD domain containing 4; mutation 1, Bruce Beutler
Synonyms: inwood
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
Nlrc4
Name: NLR family, CARD domain containing 4
Synonyms: Ipaf, Card12, 9530011P19Rik
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 268973
VEGA: 17
Homologene: 10924
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Genetic Alterations
ENU-induced T to C transversion at base pair 74,445,630 (v38) on chromosome 17, or base pair 13,514 in the GenBank genomic region NC_000083 for the Nlrc4 gene. The mutation corresponds to residue 1,973 in the mRNA sequence NM_001033367 within exon 4 of 9 total exons. The mutation results in an isoleucine (I) to threonine (T) substitution at position 586 (I586T) in the NLRC4 protein.

Strain Description

Phenotype

Homozygous: Mice exhibited impaired peritoneal macrophage inflammatory responses (i.e., decreased secretion of the proinflammatory cytokine interleukin (IL)-1β) in response to priming with flagellin.

Heterozygous: Mice exhibited slightly impaired peritoneal macrophage inflammatory responses (i.e., decreased secretion of the proinflammatory cytokine interleukin (IL)-1β) in response to priming with flagellin compared to wild-type mice.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
    • decreased interleukin-1 beta secretion [MP:0008658]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation

Strain Origin

Primary Reference

Shi H, Wang Y, Zhang Z, Dao D, Sun L, Murray AR, Beutler B. Record for inwood. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

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