Strain Detail Sheet

Strain Name:
C57BL/6J-Prkdcm2Btlr/Mmmh
Stock Number:
038256--TBD-
Citation ID:
RRID:MMRRC_038256--TBD-
Other Names:
screamer
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Prkdcm2Btlr
Name: protein kinase, DNA activated, catalytic polypeptide; mutation 2, Bruce Beutler
Synonyms: screamer
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
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Genetic Alterations
ENU-induced G to T transversion at base pair 15,831,282 (v38) on chromosome 16, equivalent to base pair 193,417 in the GenBank genomic region NC_000082 encoding the Prkdc gene. The mutation corresponds to residue 11,142 in the NM_011159 mRNA sequence in exon 78 of 86 total exons. The mutation results in a glycine (G) to premature stop codon (*) substitution at amino acid 3707 in the DNA-PKCS protein. The effect of the mutation in the Prkdc mRNA and the encoded protein has not been determined.

Strain Description

Phenotype

Homozygous: Homozygous screamer mice lack T-dependent IgG responses to both aluminum hydroxide (alum)-emulsified ovalbumin (OVA) and recombinant Semliki Forest virus (rSFV)-β-galactosidase as well as a T-independent IgM response to NP-Ficoll. Flow cytometric analysis of peripheral blood determined that the screamer mice exhibit a strong deficiency in B cells, CD4+ T cells, and CD8+ T cells.

Heterozygous: Not examined.

Mammalian Phenotype Terms
Allelic Composition: Prkdcm2Btlr/Prkdcm2Btlr (Genetic Background: C57BL/6J-Prkdcm2Btlr )
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Cell Biology
  • Immunology and Inflammation
  • Models for Human Disease
    • Severe combined immunodeficiency (SCID)

Strain Origin

Primary Reference

Wang K-W, Zeng M, Choi JH, Murray AR, Beutler B .Record for screamer. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

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Fees

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