Strain Name:
C57BL/6J-MtgxR1135Btlr/Mmmh
Stock Number:
039208-MU
Citation ID:
RRID:MMRRC_039208-MU
Other Names:
R1135 (G1), C57BL/6J-MtgxR1135Btlr
Major Collection:

Strain Information

Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Cemip2
Name: cell migration inducing hyaluronidase 2
Synonyms: 3110012M15Rik, Tmem2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 83921
VEGA: 19
Homologene: 75008
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: nonmuscle myosin heavy chain IIB, SMemb, NMHC-B, nonmuscle myosin heavy chain II-B, Myhn-2, 5730504C04Rik, myosin IIB, Fltn, Myhn2, Fltn, NMHC II-B, Myosin IIB, 9330167F11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Rab3gap2
Name: RAB3 GTPase activating protein subunit 2
Synonyms: 1110059F07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98732
Homologene: 40842
Larp4
Name: La ribonucleoprotein 4
Synonyms: D330037H05Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 207214
VEGA: 15
Homologene: 19173
Tbck
Name: TBC1 domain containing kinase
Synonyms: 1700120J03Rik, A630047E20Rik, C030007I09Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 271981
Homologene: 13221
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230895
Homologene: 15583
Srsf4
Name: serine and arginine-rich splicing factor 4
Synonyms: MNCb-2616, Sfrs4, SRp75, 5730499P16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 57317
Homologene: 25624
Msantd2
Name: Myb/SANT-like DNA-binding domain containing 2
Synonyms: 2810450G17Rik, BC024479, 9530092B10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235184
Homologene: 36421
Lct
Name: lactase
Synonyms: LOC226413, LPH, Lphl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226413
HGNC: HGNC:6530
Homologene: 124204
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Cola2, Cola-2, Col1a-2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Ints11
Name: integrator complex subunit 11
Synonyms: 2410006F12Rik, Cpsf3l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 71957
Homologene: 9894
Dock10
Name: dedicator of cytokinesis 10
Synonyms: A630054M16Rik, Zizimin3, Jr5, Jr4, ZIZ3, 9330153B10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 210293
Homologene: 45952
Wnk2
Name: WNK lysine deficient protein kinase 2
Synonyms: X83337, ESTM15, 1810073P09Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75607
Homologene: 19155
Skint10
Name: selection and upkeep of intraepithelial T cells 10
Synonyms: A030001H23Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230613
Homologene: 136292
Rpap2
Name: RNA polymerase II associated protein 2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231571
Homologene: 11733
Sytl1
Name: synaptotagmin-like 1
Synonyms: PSGL-1, Slp1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269589
Homologene: 12853
Nox4
Name: NADPH oxidase 4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 50490
HGNC: HGNC:7891
Homologene: 41065
Cr2
Name: complement receptor 2
Synonyms: CD35, Cr-2, Cr1, C3DR, CD21, Cr-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12902
HGNC: HGNC:2336
Homologene: 55611
Fibin
Name: fin bud initiation factor homolog (zebrafish)
Synonyms: 1110018M03Rik, Fibin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67606
Homologene: 12163
Trhr2
Name: thyrotropin releasing hormone receptor 2
Synonyms: TRH-R2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 170732
Homologene: 44052
Ak5
Name: adenylate kinase 5
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229949
HGNC: HGNC:365
Homologene: 76103
Nkx2-4
Name: NK2 homeobox 4
Synonyms: 1700001P03Rik, Nkx-2.4, tinman
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228731
HGNC: HGNC:7837
Homologene: 41526
2510039O18Rik
Name: RIKEN cDNA 2510039O18 gene
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 77034
Homologene: 12668
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 80,567,281 bp
  • A to G, chromosome 1 at 128,294,124 bp
  • G to A, chromosome 1 at 185,275,943 bp
  • A to T, chromosome 1 at 195,157,190 bp
  • G to A, chromosome 2 at 110,362,222 bp
  • G to T, chromosome 2 at 147,084,408 bp
  • T to A, chromosome 3 at 132,732,191 bp
  • T to A, chromosome 3 at 152,653,662 bp
  • C to T, chromosome 4 at 112,711,463 bp
  • T to G, chromosome 4 at 131,900,069 bp
  • C to T, chromosome 4 at 133,256,970 bp
  • T to C, chromosome 4 at 145,155,589 bp
  • G to T, chromosome 4 at 147,944,658 bp
  • T to C, chromosome 4 at 155,887,927 bp
  • A to T, chromosome 5 at 107,607,002 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • C to T, chromosome 7 at 87,323,789 bp
  • A to G, chromosome 8 at 122,358,633 bp
  • A to G, chromosome 9 at 37,522,712 bp
  • A to T, chromosome 11 at 68,807,197 bp
  • G to A, chromosome 13 at 49,076,558 bp
  • T to A, chromosome 15 at 99,986,204 bp
  • T to A, chromosome 18 at 60,957,396 bp
  • T to G, chromosome 19 at 21,856,142 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1135 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039208-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.