Strain Name:
C57BL/6J-MtgxR2196Btlr/Mmmh
Stock Number:
040198-MU
Citation ID:
RRID:MMRRC_040198-MU
Other Names:
R2196 (G1), C57BL/6J-MtgxR2196Btlr
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: PC-1, PC1, polycystin-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: Gbeta5, G beta 5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14697
VEGA: 9
HGNC: HGNC:4401
Homologene: 40714
Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: E430025J12Rik, Nedd4-1, Nedd4, Nedd4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Nrxn2
Name: neurexin II
Synonyms: neurexin II beta, 6430591O13Rik, neurexin II alpha
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18190
HGNC: HGNC:8009
Homologene: 86984
Acvr2a
Name: activin receptor IIA
Synonyms: Acvr2, tActRII, ActRIIa
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11480
HGNC: HGNC:173
Homologene: 20391
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: AIP4, 6720481N21Rik, C230047C07Rik, 8030492O04Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16396
Homologene: 88442
Cep55
Name: centrosomal protein 55
Synonyms: 2700032M20Rik, 1200008O12Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74107
VEGA: 19
HGNC: HGNC:1161
Homologene: 10019
Map4
Name: microtubule-associated protein 4
Synonyms: MAP 4, Mtap4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Ythdf3
Name: YTH N6-methyladenosine RNA binding protein 3
Synonyms: 9130022A11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229096
Homologene: 34991
Zfhx3
Name: zinc finger homeobox 3
Synonyms: A230102L03Rik, Atbf1, Sci, WBP9
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Thoc1
Name: THO complex 1
Synonyms: 3110002N20Rik, NMP-84
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225160
VEGA: 18
Homologene: 38012
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Urb1
Name: URB1 ribosome biogenesis 1 homolog (S. cerevisiae)
Synonyms: 5730405K23Rik, 4921511H13Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207932
Homologene: 45941
Csad
Name: cysteine sulfinic acid decarboxylase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 246277
VEGA: 15
Homologene: 41086
Dars1
Name: aspartyl-tRNA synthetase 1
Synonyms: 5730439G15Rik, Dars
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226414
HGNC: HGNC:2678
Homologene: 1032
Scn10a
Name: sodium channel, voltage-gated, type X, alpha
Synonyms: Nav1.8
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20264
VEGA: 9
Homologene: 21300
Grin2c
Name: glutamate receptor, ionotropic, NMDA2C (epsilon 3)
Synonyms: NR2C, NMDAR2C, GluRepsilon3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14813
HGNC: HGNC:4587
Homologene: 647
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Notch1
Name: notch 1
Synonyms: 9930111A19Rik, lin-12, N1, Motch A, Mis6, Tan1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18128
HGNC: HGNC:7881
Homologene: 32049
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Zfp644
Name: zinc finger protein 644
Synonyms: 1110068L01Rik, D5Ertd689e, Zep-2, BM-005
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52397
Homologene: 12971
Apba3
Name: amyloid beta precursor protein binding family A member 3
Synonyms: Mint-3, neuronal munc18-1-interacting protein 3, neuron-specific X11L2 protein, X11gamma, Mint 3, lin-10
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 57267
VEGA: 10
HGNC: HGNC:580
Homologene: 3591
Emc1
Name: ER membrane protein complex subunit 1
Synonyms: C230096C10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230866
Homologene: 9002
Pcolce2
Name: procollagen C-endopeptidase enhancer 2
Synonyms: 2400001O18Rik, Pcpe2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76477
HGNC: HGNC:8739
Homologene: 8357
Zp2
Name: zona pellucida glycoprotein 2
Synonyms: Zp-2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22787
Homologene: 48194
Enam
Name: enamelin
Synonyms: abte
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13801
HGNC: HGNC:3344
Homologene: 9698
Myo15a
Name: myosin XVA
Synonyms: Myo15
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17910
HGNC: HGNC:7594
Homologene: 56504
Plekhg4
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 4
Synonyms: 4931414L13Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102075
Homologene: 18516
Cngb3
Name: cyclic nucleotide gated channel beta 3
Synonyms: Cngbeta2, CNG6, CCNC2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 30952
HGNC: HGNC:2153
Homologene: 40908
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329628
Homologene: 14377
Tnn
Name: tenascin N
Synonyms: tenascin-W, Tnw
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329278
Homologene: 18634
Or6d14
Name: olfactory receptor family 6 subfamily D member 14
Synonyms: MOR119-1, GA_x54KRFPKN04-58190962-58191900, Olfr214
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 258754
Homologene: 133694
Fbxl4
Name: F-box and leucine-rich repeat protein 4
Synonyms: FBL5, FBL4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269514
Homologene: 8128
Actn2
Name: actinin alpha 2
Synonyms: 1110008F24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 11472
HGNC: HGNC:164
Homologene: 31016
Ankhd1
Name: ankyrin repeat and KH domain containing 1
Synonyms: 9130019P20Rik, 4933432B13Rik, A530027J04Rik, 1110004O12Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 108857
Homologene: 87006
Cacna1b
Name: calcium channel, voltage-dependent, N type, alpha 1B subunit
Synonyms: Cchn1a, alpha(1B), Cav2.2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12287
HGNC: HGNC:1389
Homologene: 20184
Vmn2r93
Name: vomeronasal 2, receptor 93
Synonyms: EG627132
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627132
Homologene: 129750
Ghrhr
Name: growth hormone releasing hormone receptor
Synonyms: Ghrfr
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14602
HGNC: HGNC:4266
Homologene: 640
Serpina1c
Name: serine (or cysteine) peptidase inhibitor, clade A, member 1C
Synonyms: PI6, PI3, Spi1-6, Spi1-3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20702
HGNC: HGNC:8941
Homologene: 20103
Spag16
Name: sperm associated antigen 16
Synonyms: 4921511D23Rik, 4930585K05Rik, 4930524F24Rik, Pf20, Wdr29
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 66722
Homologene: 11572
Or1j12
Name: olfactory receptor family 1 subfamily J member 12
Synonyms: Olfr340, MOR136-1, GA_x6K02T2NLDC-33147742-33148680
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258953
Homologene: 74159
Nup210
Name: nucleoporin 210
Synonyms: gp190, gp210, Pom210
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 54563
Homologene: 41286
Slc29a4
Name: solute carrier family 29 (nucleoside transporters), member 4
Synonyms: mPMAT, ENT4
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243328
Homologene: 71345
Krt87
Name: keratin 87
Synonyms: Krt2-25, Krt83
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 406219
VEGA: 15
Homologene: 133804
Spata22
Name: spermatogenesis associated 22
Synonyms: LOC380709
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380709
Homologene: 13076
Usp4
Name: ubiquitin specific peptidase 4 (proto-oncogene)
Synonyms: F730026I20Rik, Unp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22258
Homologene: 20716
Bank1
Name: B cell scaffold protein with ankyrin repeats 1
Synonyms: A530094C12Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242248
Homologene: 9926
Slc26a7
Name: solute carrier family 26, member 7
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 208890
Homologene: 13770
Or4p21
Name: olfactory receptor family 4 subfamily P member 21
Synonyms: MOR225-8P, MOR225-7P, Olfr1182, MOR225-8P, Gm13757, Olfr1536-ps1, MOR225-16_p, GA_x6K02T2Q125-49934557-49933640
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258167
Homologene: 86666
Trpc4
Name: transient receptor potential cation channel, subfamily C, member 4
Synonyms: STRPC4, CCE1, Trrp4, Trp4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 22066
Homologene: 22955
Bnipl
Name: BCL2/adenovirus E1B 19kD interacting protein like
Synonyms: BNIPL2, PP753, BNIPL-1, BNIPL, BNIP-S, BNIPL-2, 1700128A13Rik, BNIPL1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 171388
Homologene: 15900
Nom1
Name: nucleolar protein with MIF4G domain 1
Synonyms: Gm1040, D5Kng1, LOC381627
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 433864
Homologene: 39776
Cep112
Name: centrosomal protein 112
Synonyms: 1700001M19Rik, 1700029K01Rik, Ccdc46, 8430407H02Rik, Macoco
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76380
Homologene: 44915
Mkx
Name: mohawk homeobox
Synonyms: Irxl1, 9430023B20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 210719
Homologene: 72239
Sult2a8
Name: sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 8
Synonyms: mL-STL, 2810007J24Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76971
Rab3a
Name: RAB3A, member RAS oncogene family
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19339
HGNC: HGNC:9777
Homologene: 20629
Gm5431
Name: predicted gene 5431
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 432555
Cyb5rl
Name: cytochrome b5 reductase-like
Synonyms: 2810410C14Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230582
Homologene: 45506
Gcsh
Name: glycine cleavage system protein H (aminomethyl carrier)
Synonyms: 1100001L02Rik, 5730591C18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68133
HGNC: HGNC:4208
Homologene: 90880
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 69,858,522 bp
  • A to T, chromosome 1 at 128,378,858 bp
  • G to C, chromosome 1 at 160,097,228 bp
  • C to T, chromosome 2 at 24,761,788 bp
  • A to T, chromosome 2 at 26,463,804 bp
  • T to A, chromosome 2 at 36,452,588 bp
  • A to T, chromosome 2 at 48,870,312 bp
  • G to A, chromosome 2 at 88,446,710 bp
  • A to C, chromosome 2 at 155,202,221 bp
  • T to C, chromosome 3 at 16,203,818 bp
  • T to C, chromosome 3 at 38,981,417 bp
  • A to G, chromosome 3 at 54,302,193 bp
  • T to A, chromosome 3 at 95,249,870 bp
  • A to T, chromosome 3 at 136,284,266 bp
  • T to A, chromosome 4 at 14,593,806 bp
  • T to C, chromosome 4 at 19,415,690 bp
  • T to C, chromosome 4 at 22,403,624 bp
  • T to C, chromosome 4 at 107,071,033 bp
  • A to T, chromosome 4 at 139,366,530 bp
  • A to G, chromosome 5 at 29,436,021 bp
  • A to G, chromosome 5 at 88,502,744 bp
  • A to T, chromosome 5 at 106,638,603 bp
  • A to C, chromosome 5 at 142,712,895 bp
  • T to C, chromosome 6 at 55,379,741 bp
  • C to A, chromosome 6 at 85,667,963 bp
  • A to T, chromosome 6 at 91,055,244 bp
  • A to T, chromosome 6 at 116,556,617 bp
  • T to A, chromosome 7 at 14,427,853 bp
  • T to C, chromosome 7 at 120,138,306 bp
  • T to A, chromosome 8 at 45,024,646 bp
  • T to C, chromosome 8 at 70,757,226 bp
  • TAGTCGATGCCCGAGTC to TAGTC, chromosome 8 at 105,376,452 bp
  • G to T, chromosome 8 at 108,800,253 bp
  • G to A, chromosome 8 at 116,989,170 bp
  • C to T, chromosome 9 at 72,725,074 bp
  • A to T, chromosome 9 at 75,327,229 bp
  • A to T, chromosome 9 at 95,694,689 bp
  • A to G, chromosome 9 at 108,373,686 bp
  • A to G, chromosome 9 at 110,071,048 bp
  • G to A, chromosome 9 at 119,609,004 bp
  • C to T, chromosome 9 at 119,617,670 bp
  • A to G, chromosome 10 at 81,271,708 bp
  • A to T, chromosome 11 at 48,889,231 bp
  • T to A, chromosome 11 at 60,510,021 bp
  • A to G, chromosome 11 at 73,345,834 bp
  • G to A, chromosome 11 at 108,570,361 bp
  • G to T, chromosome 11 at 115,250,666 bp
  • T to A, chromosome 12 at 103,896,111 bp
  • T to A, chromosome 13 at 12,275,179 bp
  • T to C, chromosome 15 at 101,438,433 bp
  • T to C, chromosome 15 at 102,187,593 bp
  • A to G, chromosome 16 at 90,774,256 bp
  • C to T, chromosome 17 at 18,305,166 bp
  • T to C, chromosome 17 at 24,580,072 bp
  • A to G, chromosome 18 at 7,000,675 bp
  • T to A, chromosome 18 at 9,986,300 bp
  • T to A, chromosome 18 at 36,648,379 bp
  • T to A, chromosome 18 at 49,917,631 bp
  • A to T, chromosome 19 at 6,490,109 bp
  • T to A, chromosome 19 at 38,069,110 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2196 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040198-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.