Strain Name:
C57BL/6J-MtgxR3003Btlr/Mmmh
Stock Number:
040532-MU
Citation ID:
RRID:MMRRC_040532-MU
Other Names:
R3003 (G1), C57BL/6J-MtgxR3003Btlr
Major Collection:

Strain Information

Suz12
Name: SUZ12 polycomb repressive complex 2 subunit
Synonyms: D11Ertd530e, 2610028O16Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 52615
Homologene: 32256
Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: Rap, 4932417H02Rik, raptor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210
Eprs1
Name: glutamyl-prolyl-tRNA synthetase 1
Synonyms: 3010002K18Rik, Qprs, 2410081F06Rik, Eprs
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 107508
HGNC: HGNC:3418
Homologene: 5870
Acly
Name: ATP citrate lyase
Synonyms: A730098H14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 104112
HGNC: HGNC:115
Homologene: 854
Tut4
Name: terminal uridylyl transferase 4
Synonyms: Tent3a, 9230115F04Rik, Zcchc11, 6030404K05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230594
Homologene: 35279
Clptm1l
Name: CLPTM1-like
Synonyms: C130052I12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218335
VEGA: 13
Homologene: 12767
Rnf17
Name: ring finger protein 17
Synonyms: MMIP-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 30054
VEGA: 14
Homologene: 23727
Slitrk5
Name: SLIT and NTRK-like family, member 5
Synonyms: 2610019D03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 75409
VEGA: 14
Homologene: 9193
Klk11
Name: kallikrein related-peptidase 11
Synonyms: hippostasin, 2310015I08Rik, TLSP, Prss20
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56538
HGNC: HGNC:6359
Homologene: 27048
Tmem81
Name: transmembrane protein 81
Synonyms: 4930429O20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74626
Homologene: 12579
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94109
Homologene: 69536
Lct
Name: lactase
Synonyms: LOC226413, LPH, Lphl
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226413
HGNC: HGNC:6530
Homologene: 124204
Smarcd1
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
Synonyms: Baf60a, D15Kz1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 83797
VEGA: 15
Homologene: 20670
Stat4
Name: signal transducer and activator of transcription 4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20849
Homologene: 20679
Sycp2
Name: synaptonemal complex protein 2
Synonyms: 3830402K23Rik, 4930518F03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320558
Homologene: 8604
Atg9b
Name: autophagy related 9B
Synonyms: Nos3as, LOC213948, Apg9l2, eONE
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 213948
Homologene: 72638
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: E230008N13Rik, LOC381522
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 381522
Homologene: 117988
Mprip
Name: myosin phosphatase Rho interacting protein
Synonyms: Rhoip3, Gm34094, RIP3, p116Rip, p116 Rho interacting protein
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26936
Homologene: 9034
Vmn2r98
Name: vomeronasal 2, receptor 98
Synonyms: EG224552
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224552
Homologene: 115024
Pasd1
Name: PAS domain containing repressor 1
Synonyms: Gm1141, LOC382221
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 382221
Il27ra
Name: interleukin 27 receptor, alpha
Synonyms: Tccr, WSX-1, IL-27R
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50931
Homologene: 3562
Plxnc1
Name: plexin C1
Synonyms: 2510048K12Rik, CD232, vespr
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 54712
HGNC: HGNC:9106
Homologene: 4211
Pfpl
Name: pore forming protein-like
Synonyms: Epcs5, Epcs50
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 56093
VEGA: 19
Homologene: 130704
Abcc1
Name: ATP-binding cassette, sub-family C member 1
Synonyms: Mrp1, Abcc1a, Mdrap, MRP, Abcc1b
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17250
HGNC: HGNC:51
Homologene: 133779
Gm4846
Name: predicted gene 4846
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226601
Homologene: 136281
Adh6b
Name: alcohol dehydrogenase 6B (class V)
Synonyms: Adh5b
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100417514
HGNC: HGNC:255
Homologene: 139477
Acd
Name: adrenocortical dysplasia
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 497652
Homologene: 23391
Cep112
Name: centrosomal protein 112
Synonyms: 1700001M19Rik, 1700029K01Rik, Ccdc46, 8430407H02Rik, Macoco
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76380
Homologene: 44915
Arhgap8
Name: Rho GTPase activating protein 8
Synonyms: 3110043J09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 73167
Homologene: 23645
Tmem181a
Name: transmembrane protein 181A
Synonyms: Tmem181, Gpr178, 5930418K15Rik, C76977
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 77106
Homologene: 44787
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 52,102,986 bp
  • T to C, chromosome 1 at 128,304,226 bp
  • A to G, chromosome 1 at 132,508,014 bp
  • T to C, chromosome 1 at 166,486,878 bp
  • T to C, chromosome 1 at 185,424,391 bp
  • T to C, chromosome 2 at 178,358,123 bp
  • G to T, chromosome 3 at 138,357,771 bp
  • A to G, chromosome 4 at 45,899,988 bp
  • G to A, chromosome 4 at 108,512,928 bp
  • G to T, chromosome 5 at 24,391,219 bp
  • T to C, chromosome 7 at 43,776,995 bp
  • A to G, chromosome 8 at 16,196,170 bp
  • G to T, chromosome 8 at 84,032,031 bp
  • C to T, chromosome 8 at 105,700,281 bp
  • A to T, chromosome 10 at 94,793,218 bp
  • A to G, chromosome 11 at 59,727,555 bp
  • T to A, chromosome 11 at 80,019,761 bp
  • T to C, chromosome 11 at 100,504,227 bp
  • A to G, chromosome 11 at 108,440,503 bp
  • G to A, chromosome 11 at 119,872,371 bp
  • C to T, chromosome 13 at 73,617,756 bp
  • T to C, chromosome 14 at 56,500,547 bp
  • A to G, chromosome 14 at 111,679,582 bp
  • G to T, chromosome 15 at 84,771,830 bp
  • C to A, chromosome 15 at 99,712,184 bp
  • T to C, chromosome 16 at 14,436,529 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • A to G, chromosome 17 at 19,065,863 bp
  • T to C, chromosome 19 at 12,430,326 bp
  • GCCCCCC to GCCCCCCCCCC, chromosome X at 71,940,209 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3003 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040532-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.