Strain Name:
C57BL/6J-MtgxR3120Btlr/Mmmh
Stock Number:
040593-MU
Citation ID:
RRID:MMRRC_040593-MU
Other Names:
R3120 (G1), C57BL/6J-MtgxR3120Btlr
Major Collection:

Strain Information

Polr2f
Name: polymerase (RNA) II (DNA directed) polypeptide F
Synonyms: RNA Pol II, 1810060D16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69833
VEGA: 15
HGNC: HGNC:9193
Homologene: 7178
Ptdss2
Name: phosphatidylserine synthase 2
Synonyms: PSS2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27388
Homologene: 8462
Rlf
Name: rearranged L-myc fusion sequence
Synonyms: 9230110M18Rik, MommeD8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109263
Homologene: 8243
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Sfpq
Name: splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)
Synonyms: D4Ertd314e, 9030402K04Rik, 2810416M14Rik, 5730453G22Rik, 1110004P21Rik, REP1, PSF
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 71514
Homologene: 3714
Xrn2
Name: 5'-3' exoribonuclease 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 24128
Homologene: 6927
Tbcd
Name: tubulin-specific chaperone d
Synonyms: A030005L14Rik, 2310057L06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 108903
Homologene: 4368
Plekha5
Name: pleckstrin homology domain containing, family A member 5
Synonyms: PEPP2, 2810431N21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109135
Homologene: 10377
Fam222b
Name: family with sequence similarity 222, member B
Synonyms: BC017647
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216971
Homologene: 10054
Tfap2c
Name: transcription factor AP-2, gamma
Synonyms: Ap-2.2, Stra2, AP2gamma, Tcfap2c
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21420
Homologene: 2423
Bsx
Name: brain specific homeobox
Synonyms: Bsx1b, Bsx1a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244813
Homologene: 65347
Gclc
Name: glutamate-cysteine ligase, catalytic subunit
Synonyms: gamma GCS-HS, Glclc, GLCL-H, D9Wsu168e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14629
HGNC: HGNC:4311
Homologene: 1148
Ccdc25
Name: coiled-coil domain containing 25
Synonyms: 2610528H13Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67179
VEGA: 14
Homologene: 6645
Nup58
Name: nucleoporin 58
Synonyms: 1700017F11Rik, Nupl1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71844
VEGA: 14
Homologene: 40924
Mis18bp1
Name: MIS18 binding protein 1
Synonyms: C79407
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217653
Homologene: 10147
Tubgcp3
Name: tubulin, gamma complex component 3
Synonyms: Spc98p, GCP3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 259279
Homologene: 4609
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, E030034C22Rik, B230373P09Rik, Dnahc1, G1-415-19, ferf1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Hmcn2
Name: hemicentin 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 665700
Homologene: 90772
Mfsd12
Name: major facilitator superfamily domain containing 12
Synonyms: Wdt1, F630110N24Rik, gr
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 73822
Homologene: 45488
Sybu
Name: syntabulin (syntaxin-interacting)
Synonyms: A830027B17Rik, Golsyn/Syntabulin, 5730410E15Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 319613
VEGA: 15
Homologene: 9838
Prph2
Name: peripherin 2
Synonyms: rds, Tspan22, Rd2, Nmf193
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19133
HGNC: HGNC:9942
Homologene: 273
Eml1
Name: echinoderm microtubule associated protein like 1
Synonyms: ELP79, 1110008N23Rik, A930030P13Rik, heco
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68519
HGNC: HGNC:3330
Homologene: 20931
Ccdc39
Name: coiled-coil domain containing 39
Synonyms: 4921507O14Rik, D3Ertd789e, b2b1735Clo, b2b1304Clo, b2b2025.1Clo, prh
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 51938
Homologene: 12149
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 81877
Homologene: 49589
Scgb2b2
Name: secretoglobin, family 2B, member 2
Synonyms: Abpe, Abpbg2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381970
Homologene: 83171
Slc4a10
Name: solute carrier family 4, sodium bicarbonate cotransporter-like, member 10
Synonyms: NCBE
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94229
Homologene: 23340
Taar7f
Name: trace amine-associated receptor 7F
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 435207
Homologene: 134040
Trim28
Name: tripartite motif-containing 28
Synonyms: KRIP-1, KAP-1, Tif1b, MommeD9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21849
Homologene: 21175
Rpl10a-ps2
Name: ribosomal protein L10A, pseudogene 2
Synonyms: Gm5192
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 382723
Or5p70
Name: olfactory receptor family 5 subfamily P member 70
Synonyms: GA_x6K02T2PBJ9-10725148-10726140, MOR204-37, Olfr495
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258361
Homologene: 133600
Vmn2r14
Name: vomeronasal 2, receptor 14
Synonyms: EG231591
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231591
Homologene: 129606
Pkdcc
Name: protein kinase domain containing, cytoplasmic
Synonyms: ESTM17, Vlk, MAd1, Adtk1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106522
Homologene: 18932
Nlrp4f
Name: NLR family, pyrin domain containing 4F
Synonyms: Nalp-kappa, C330026N02Rik, Nalp4f
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 97895
VEGA: 13
Homologene: 87252
Fars2
Name: phenylalanine-tRNA synthetase 2, mitochondrial
Synonyms: 2810431B21Rik, 6720478K01Rik, Fars1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69955
VEGA: 13
Homologene: 4788
St6gal2
Name: beta galactoside alpha 2,6 sialyltransferase 2
Synonyms: C230064G14Rik, ST6Gal II
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240119
Homologene: 13052
Gm4846
Name: predicted gene 4846
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226601
Homologene: 136281
H1f5
Name: H1.5 linker histone, cluster member
Synonyms: H1B, H1.5, H1s-3, H1f5, Hist1h1b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56702
HGNC: HGNC:4719
Homologene: 135981
Gatad1
Name: GATA zinc finger domain containing 1
Synonyms: 2810047M21Rik, 2310031E19Rik, B330017N08Rik, 8430439A17Rik, Odag, 9130430G15Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67210
Homologene: 56916
Syt15
Name: synaptotagmin XV
Synonyms: CHR10SYT, sytXV, E230025K04Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319508
Homologene: 12901
Hbq1a
Name: hemoglobin, theta 1A
Synonyms: LOC380691, A230090E05Rik, Hbq1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216635
HGNC: HGNC:4833
Crygs
Name: crystallin, gamma S
Synonyms: Opj
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12970
VEGA: 16
HGNC: HGNC:2417
Homologene: 40695
Zfp551
Name: zinc finger protein 551
Synonyms: 9630004E07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 619331
Homologene: 64631
Magea6
Name: MAGE family member A6
Synonyms: Mage-a6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 17142
Homologene: 23188
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 166,491,548 bp
  • G to A, chromosome 2 at 31,452,799 bp
  • A to G, chromosome 2 at 62,298,233 bp
  • A to T, chromosome 2 at 147,038,158 bp
  • G to A, chromosome 2 at 172,557,095 bp
  • T to C, chromosome 3 at 33,837,838 bp
  • T to A, chromosome 4 at 121,149,483 bp
  • T to A, chromosome 4 at 127,022,133 bp
  • G to T, chromosome 5 at 3,641,456 bp
  • A to T, chromosome 5 at 109,224,565 bp
  • A to G, chromosome 6 at 140,591,641 bp
  • A to G, chromosome 7 at 12,416,016 bp
  • A to G, chromosome 7 at 13,028,414 bp
  • T to C, chromosome 7 at 31,303,576 bp
  • T to C, chromosome 7 at 108,395,723 bp
  • C to T, chromosome 7 at 141,152,219 bp
  • G to A, chromosome 8 at 12,657,626 bp
  • A to G, chromosome 9 at 40,877,612 bp
  • A to C, chromosome 9 at 77,781,270 bp
  • C to A, chromosome 10 at 24,049,580 bp
  • T to G, chromosome 10 at 81,361,215 bp
  • T to C, chromosome 11 at 32,300,472 bp
  • T to C, chromosome 11 at 78,153,916 bp
  • C to A, chromosome 11 at 79,564,899 bp
  • T to C, chromosome 11 at 121,608,648 bp
  • T to C, chromosome 12 at 65,156,988 bp
  • A to G, chromosome 12 at 108,513,053 bp
  • A to T, chromosome 13 at 8,940,650 bp
  • A to T, chromosome 13 at 21,780,045 bp
  • A to G, chromosome 13 at 36,246,417 bp
  • T to C, chromosome 13 at 65,194,716 bp
  • G to A, chromosome 14 at 31,266,822 bp
  • A to G, chromosome 14 at 34,222,993 bp
  • A to G, chromosome 14 at 60,232,674 bp
  • A to G, chromosome 14 at 65,856,911 bp
  • T to C, chromosome 15 at 44,672,959 bp
  • T to A, chromosome 15 at 79,144,588 bp
  • C to T, chromosome 16 at 22,805,551 bp
  • C to T, chromosome 17 at 34,692,355 bp
  • C to T, chromosome 17 at 46,923,372 bp
  • A to T, chromosome 17 at 55,482,110 bp
  • T to C, chromosome 17 at 83,220,037 bp
  • A to T, chromosome X at 154,924,295 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3120 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040593-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.