Strain Name:
C57BL/6J-MtgxR4230Btlr/Mmmh
Stock Number:
041049-MU
Citation ID:
RRID:MMRRC_041049-MU
Other Names:
R4230 (G1), C57BL/6J-MtgxR4230Btlr
Major Collection:

Strain Information

Cadps
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 27062
VEGA: 14
HGNC: HGNC:1426
Homologene: 2755
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Jam2
Name: junction adhesion molecule 2
Synonyms: 2410030G21Rik, JAM-2, VE-JAM, 2410167M24Rik, Jcam2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67374
Homologene: 10929
Ncoa7
Name: nuclear receptor coactivator 7
Synonyms: 9030406N13Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 211329
VEGA: 10
Homologene: 65245
Pds5a
Name: PDS5 cohesin associated factor A
Synonyms: E230024D05Rik, 9030416H16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71521
Homologene: 22877
Sf3a2
Name: splicing factor 3a, subunit 2
Synonyms: SFA66, PRP11, Sap62, 66kDa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20222
Homologene: 133823
Cog6
Name: component of oligomeric golgi complex 6
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67542
Homologene: 10802
Kcnk5
Name: potassium channel, subfamily K, member 5
Synonyms: TASK-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16529
VEGA: 14
HGNC: HGNC:6280
Homologene: 2773
Arhgap32
Name: Rho GTPase activating protein 32
Synonyms: p200RhoGAP, GC-GAP, PX-RICS, 3426406O18Rik, Grit
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330914
Homologene: 8812
Rasa3
Name: RAS p21 protein activator 3
Synonyms: GAPIII activator 3, Ras GTPase-activating protein III, GAPIII, R-Ras gap, scat, hlb381
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19414
Homologene: 7217
Itln1
Name: intelectin 1 (galactofuranose binding)
Synonyms: IntL, Itln2, mLfR, Itlna
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16429
Homologene: 111044
Sec61a1
Name: SEC61 translocon subunit alpha 1
Synonyms: Sec61a
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 53421
Homologene: 55537
Ankrd26
Name: ankyrin repeat domain 26
Synonyms: 5730521P14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232339
Homologene: 45968
Sec24b
Name: SEC24 homolog B, COPII coat complex component
Synonyms: SEC24
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99683
Homologene: 55968
Ttn
Name: titin
Synonyms: connectin, L56, 1100001C23Rik, mdm, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Urod
Name: uroporphyrinogen decarboxylase
Synonyms: Uro-d
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22275
Homologene: 320
Tgm5
Name: transglutaminase 5
Synonyms: 2310007C07Rik, TGx
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74176
Homologene: 20899
Slc46a2
Name: solute carrier family 46, member 2
Synonyms: TSO-1C12, Ly110, 5430429N04Rik, Tscot
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 30936
Homologene: 23273
Metrn
Name: meteorin, glial cell differentiation regulator
Synonyms: 1810034B16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70083
VEGA: 17
Homologene: 11432
Astn2
Name: astrotactin 2
Synonyms: 1d8, Astnl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56079
Homologene: 77850
Vmn2r2
Name: vomeronasal 2, receptor 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100125586
Homologene: 129753
Phf11c
Name: PHD finger protein 11C
Synonyms: Gm6907
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 628705
Homologene: 87807
Atxn7
Name: ataxin 7
Synonyms: ataxin-7, Sca7, A430107N12Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 246103
VEGA: 14
Homologene: 30967
Zc3h12c
Name: zinc finger CCCH type containing 12C
Synonyms: C230027N18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244871
VEGA: 9
Homologene: 35462
Muc5b
Name: mucin 5, subtype B, tracheobronchial
Synonyms: MUC9, MUC5, 2300002I04Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74180
HGNC: HGNC:7516
Homologene: 136756
Vwa8
Name: von Willebrand factor A domain containing 8
Synonyms: 4932416F07Rik, 1300010F03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219189
VEGA: 14
Homologene: 44881
Or4k37
Name: olfactory receptor family 4 subfamily K member 37
Synonyms: GA_x6K02T2Q125-72379864-72380781, MOR248-14P, MOR248-18, Olfr1281
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 257979
Homologene: 73992
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: Ovos2, BC048546
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232400
Homologene: 45969
Or52e18
Name: olfactory receptor family 52 subfamily E member 18
Synonyms: GA_x6K02T2PBJ9-7589577-7588639, MOR32-8, Olfr670
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384703
Homologene: 105163
Hltf
Name: helicase-like transcription factor
Synonyms: P113, Snf2l3, Smarca3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20585
Homologene: 30136
Tmem229a
Name: transmembrane protein 229A
Synonyms: 6332401O19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319832
Homologene: 85312
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243369
Homologene: 45453
Apbb1
Name: amyloid beta precursor protein binding family B member 1
Synonyms: Fe65, Rir
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11785
HGNC: HGNC:581
Homologene: 898
Slc45a3
Name: solute carrier family 45, member 3
Synonyms: IPCA-6, 2210413P12Rik, Pcanap6
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 212980
HGNC: HGNC:8642
Homologene: 23813
Cfap119
Name: cilia and flagella associated protein 119
Synonyms: LOC233899, Gm166, Ccdc189
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233899
Homologene: 79950
Eme1
Name: essential meiotic structure-specific endonuclease 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268465
Homologene: 16123
Or2n1
Name: olfactory receptor family 2 subfamily N member 1
Synonyms: MOR256-5, GA_x6K02T2PSCP-2623613-2624551, Olfr134
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258829
Homologene: 110603
Alg11
Name: ALG11 alpha-1,2-mannosyltransferase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 207958
Homologene: 68893
Spag6l
Name: sperm associated antigen 6-like
Synonyms: PF16, Spag6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 50525
Homologene: 8252
Fam178b
Name: family with sequence similarity 178, member B
Synonyms: LOC381337, 1700024G10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381337
Homologene: 87288
Dppa3
Name: developmental pluripotency-associated 3
Synonyms: PGC7, 2410075G02Rik, stella
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73708
Homologene: 138189
Nxpe4
Name: neurexophilin and PC-esterase domain family, member 4
Synonyms: D930028F11Rik, Fam55d
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244853
VEGA: 9
Homologene: 83471
Ifi44l
Name: interferon-induced protein 44 like
Synonyms: NS1178, H-28, H28
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 15061
Homologene: 48468
Or2z9
Name: olfactory receptor family 2 subfamily Z member 9
Synonyms: GA_x6K02T2NUPS-231686-232630, MOR282-1, MOR282-2, Olfr373
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 258532
Homologene: 17287
Vmn1r223
Name: vomeronasal 1 receptor 223
Synonyms: Gm11330
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 100036518
Homologene: 110880
Onecut3
Name: one cut domain, family member 3
Synonyms: OC-3, Oc3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 246086
Homologene: 82258
Spi1
Name: Spi-1 proto-oncogene
Synonyms: Spi-1, Sfpi-1, Tfpu.1, PU.1, Dis-1, Tcfpu1, Sfpi1, spleen focus forming virus (SFFV) proviral integration oncogene
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20375
Homologene: 2346
Gm16638
Name: predicted gene, 16638
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102631889
Gm15881
Name: predicted gene 15881
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100271928
Gm10735
Name: predicted gene 10735
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Gm25683
Name: predicted gene, 25683
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 115486588
VEGA: 7
Eif1ad14
Name: eukaryotic translation initiation factor 1A domain containing 14
Synonyms: Gm2035
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100039076
VEGA: 12
Spag6
Name: sperm associated antigen 6
Synonyms: BC061194, Spag6l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 381350
Homologene: 133723
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 36,564,399 bp
  • T to A, chromosome 1 at 131,981,661 bp
  • G to A, chromosome 1 at 171,534,807 bp
  • T to A, chromosome 2 at 18,715,638 bp
  • T to C, chromosome 2 at 76,724,120 bp
  • T to C, chromosome 2 at 91,115,335 bp
  • T to G, chromosome 2 at 111,329,130 bp
  • T to C, chromosome 2 at 121,070,735 bp
  • T to C, chromosome 3 at 20,108,193 bp
  • T to C, chromosome 3 at 52,992,808 bp
  • T to A, chromosome 3 at 64,134,491 bp
  • G to T, chromosome 3 at 130,040,719 bp
  • A to T, chromosome 3 at 151,762,877 bp
  • T to C, chromosome 4 at 59,914,048 bp
  • T to G, chromosome 4 at 65,911,682 bp
  • T to A, chromosome 4 at 116,992,683 bp
  • A to G, chromosome 5 at 65,629,986 bp
  • T to A, chromosome 6 at 24,954,832 bp
  • T to C, chromosome 6 at 48,490,934 bp
  • A to T, chromosome 6 at 88,515,431 bp
  • G to C, chromosome 6 at 118,559,388 bp
  • A to T, chromosome 6 at 122,629,332 bp
  • C to T, chromosome 6 at 128,580,386 bp
  • T to C, chromosome 7 at 83,597,402 bp
  • A to T, chromosome 7 at 104,960,594 bp
  • T to C, chromosome 7 at 105,567,684 bp
  • C to T, chromosome 7 at 118,148,733 bp
  • T to A, chromosome 7 at 127,586,830 bp
  • T to A, chromosome 7 at 141,863,522 bp
  • A to G, chromosome 8 at 13,570,264 bp
  • T to A, chromosome 8 at 22,065,518 bp
  • T to C, chromosome 8 at 58,700,184 bp
  • T to C, chromosome 8 at 72,100,344 bp
  • T to C, chromosome 9 at 32,257,474 bp
  • A to G, chromosome 9 at 48,392,822 bp
  • T to A, chromosome 9 at 52,144,428 bp
  • A to T, chromosome 10 at 30,698,257 bp
  • T to C, chromosome 10 at 80,513,959 bp
  • T to C, chromosome 10 at 80,803,660 bp
  • G to A, chromosome 11 at 94,647,992 bp
  • A to T, chromosome 12 at 87,919,797 bp
  • A to T, chromosome 13 at 23,249,415 bp
  • T to C, chromosome 13 at 113,041,209 bp
  • T to A, chromosome 14 at 12,488,987 bp
  • G to A, chromosome 14 at 14,100,381 bp
  • C to A, chromosome 14 at 20,144,784 bp
  • A to T, chromosome 14 at 59,393,067 bp
  • C to T, chromosome 14 at 79,082,852 bp
  • T to A, chromosome 16 at 84,821,292 bp
  • T to C, chromosome 17 at 25,387,863 bp
  • A to G, chromosome 17 at 25,796,941 bp
  • A to C, chromosome 17 at 38,175,881 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4230 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041049-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.