Strain Name:
C57BL/6J-MtgxR4306Btlr/Mmmh
Stock Number:
041092-MU
Citation ID:
RRID:MMRRC_041092-MU
Other Names:
R4306 (G1), C57BL/6J-MtgxR4306Btlr
Major Collection:

Strain Information

Gpm6a
Name: glycoprotein m6a
Synonyms: Gpm6, M6A
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234267
HGNC: HGNC:4460
Homologene: 3864
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: G430090F17Rik, LOC328902
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Trip11
Name: thyroid hormone receptor interactor 11
Synonyms: GMAP-210, TRIP230, 3110031G15Rik, 6030460N08Rik, 2610511G22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 109181
Homologene: 20897
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, AnkG, 2900054D09Rik, Ankyrin-3, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Fzd7
Name: frizzled class receptor 7
Synonyms: Fz7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14369
HGNC: HGNC:4045
Homologene: 20751
Slit2
Name: slit guidance ligand 2
Synonyms: Drad-1, E130320P19Rik, E030015M03Rik, Slil3, b2b1200.1Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20563
Homologene: 3516
Chrdl2
Name: chordin-like 2
Synonyms: Chl2, 1810022C01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69121
Homologene: 128795
Rb1
Name: RB transcriptional corepressor 1
Synonyms: Rb-1, pRb, Rb, retinoblastoma 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19645
HGNC: HGNC:9884
Homologene: 272
Zfp459
Name: zinc finger protein 459
Synonyms: 9930025G17Rik, Rslcan-14
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 328274
Adam15
Name: ADAM metallopeptidase domain 15
Synonyms: MDC15, metargidin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11490
HGNC: HGNC:193
Homologene: 2829
H2-K1
Name: histocompatibility 2, K1, K region
Synonyms: H-2K
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14972
Homologene: 128352
Tas2r124
Name: taste receptor, type 2, member 124
Synonyms: mGR24, T2R24, mt2r50, Tas2r24
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 387351
Homologene: 87293
Irs1
Name: insulin receptor substrate 1
Synonyms: G972R, IRS-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16367
HGNC: HGNC:6125
Homologene: 4049
Dll3
Name: delta like canonical Notch ligand 3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13389
HGNC: HGNC:2909
Homologene: 7291
Gprc6a
Name: G protein-coupled receptor, family C, group 6, member A
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 210198
VEGA: 10
Homologene: 17529
Or51ah3
Name: olfactory receptor family 51 subfamily AH member 3
Synonyms: MOR19-2, Olfr615, GA_x6K02T2PBJ9-6284902-6285843
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259084
Homologene: 17494
Frmd4a
Name: FERM domain containing 4A
Synonyms: Gm13190, 2700017I06Rik, C230040M21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 209630
Homologene: 9971
Myo1a
Name: myosin IA
Synonyms: BBM-I, brush border myosin 1, Myhl
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 432516
VEGA: 10
HGNC: HGNC:7595
Homologene: 21113
Dnah7b
Name: dynein, axonemal, heavy chain 7B
Synonyms: Dnahc7b, LOC227058
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227058
Homologene: 41287
Chst12
Name: carbohydrate sulfotransferase 12
Synonyms: C4S-2, C4ST2, C4ST-2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 59031
Homologene: 69258
Fap
Name: fibroblast activation protein
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14089
HGNC: HGNC:3590
Homologene: 48282
A830018L16Rik
Name: RIKEN cDNA A830018L16 gene
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 320492
Homologene: 14194
Usp31
Name: ubiquitin specific peptidase 31
Synonyms: 6330567E21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76179
Homologene: 44504
Vmn2r12
Name: vomeronasal 2, receptor 12
Synonyms: Gm6769
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 627569
Homologene: 129606
Rnf144b
Name: ring finger protein 144B
Synonyms: Ibrdc2, E130105P19Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218215
VEGA: 13
Homologene: 27092
Spaca7b
Name: sperm acrosome associated 7B
Synonyms: 1700016D06Rik, GC14
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76413
Cyp27b1
Name: cytochrome P450, family 27, subfamily b, polypeptide 1
Synonyms: Pddr, VddrI, P450c1, Cyp27b, Vddr, Vdd1, Cyp1, Cp2b, Cyp40, 25-hydroxyvitamin D3 1alpha-hydroxylase, P450VD1alpha, 25(OH)D 1alpha-hydroxylase, 1alpha(OH)ase
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13115
HGNC: HGNC:2606
Homologene: 37139
Naga
Name: N-acetyl galactosaminidase, alpha
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17939
VEGA: 15
HGNC: HGNC:7631
Homologene: 221
Prr27
Name: proline rich 27
Synonyms: 4930432K09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 73779
Homologene: 136277
Vmn2r71
Name: vomeronasal 2, receptor 71
Synonyms: EG233445
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233445
Homologene: 115466
Inmt
Name: indolethylamine N-methyltransferase
Synonyms: Temt
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21743
HGNC: HGNC:6069
Homologene: 81752
Gm7854
Name: predicted gene 7854
Synonyms: ENSMUSG00000054483
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 665934
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 11,972,076 bp
  • T to A, chromosome 1 at 46,221,772 bp
  • T to C, chromosome 1 at 59,484,407 bp
  • C to T, chromosome 1 at 82,287,964 bp
  • C to T, chromosome 2 at 4,333,078 bp
  • C to T, chromosome 2 at 62,530,707 bp
  • A to G, chromosome 3 at 89,345,784 bp
  • T to A, chromosome 5 at 43,235,235 bp
  • T to A, chromosome 5 at 48,302,783 bp
  • A to G, chromosome 5 at 87,842,907 bp
  • A to G, chromosome 5 at 109,086,006 bp
  • T to C, chromosome 5 at 140,524,646 bp
  • T to C, chromosome 6 at 55,173,339 bp
  • A to G, chromosome 6 at 132,754,991 bp
  • T to C, chromosome 7 at 28,301,657 bp
  • G to T, chromosome 7 at 85,624,152 bp
  • A to G, chromosome 7 at 100,022,022 bp
  • A to T, chromosome 7 at 103,561,172 bp
  • G to C, chromosome 7 at 103,561,173 bp
  • G to A, chromosome 7 at 121,706,929 bp
  • T to A, chromosome 8 at 11,678,590 bp
  • T to C, chromosome 8 at 55,047,393 bp
  • T to C, chromosome 10 at 51,616,639 bp
  • T to C, chromosome 10 at 69,882,372 bp
  • A to G, chromosome 10 at 127,051,088 bp
  • T to G, chromosome 10 at 127,714,081 bp
  • A to G, chromosome 12 at 101,886,939 bp
  • A to G, chromosome 13 at 47,242,942 bp
  • T to C, chromosome 13 at 67,413,188 bp
  • G to A, chromosome 14 at 73,262,695 bp
  • C to T, chromosome 15 at 82,336,894 bp
  • C to T, chromosome 17 at 33,999,816 bp
  • T to C, chromosome 18 at 12,819,595 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4306 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041092-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.