Strain Name:
CD1.Cg-Tg(KRT14-LMP2A)#Rlon/Mmnc
Stock Number:
041422-UNC
Citation ID:
RRID:MMRRC_041422-UNC
Other Names:
KL2, K14-LMP2A

Strain Information

KRT14
Name: keratin 14
Type: Gene
Species: Homo sapiens (human)
Chromosome: 17
LMP2A
Name: Human herpesvirus 4 (Epstein-Barr virus)
Type: Gene
Species: Homo sapiens (human)
Chromosome:
Tg(KRT14-LMP2A)#Rlon
Name: transgene insertion, Richard Longnecker
Synonyms: K14-LMP2A
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
The Epstein Barr Virus latent membrane protein 2A (LMP2A) gene is under the control of the human keratin 14 promoter.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype

    Homozygous: Undetermined

    Hemizygous: No known phenotype

    MeSH Terms
    • Animals
    • Antigens, Viral/metabolism
    • B-Lymphocytes/metabolism
    • B-Lymphocytes/virology
    • Cell Differentiation
    • Epidermis/growth & development
    • Epithelial Cells/virology
    • Herpesvirus 4, Human/metabolism
    • Keratins/genetics
    • Mice
    • Mice, Transgenic
    • Promoter Regions, Genetic
    • Signal Transduction
    • Viral Matrix Proteins/metabolism
    • 9,10-Dimethyl-1,2-benzanthracene/toxicity
    • Carcinogens
    • Carcinoma/genetics
    • Carcinoma/metabolism
    • Carcinoma/virology
    • Cell Transformation, Viral/genetics
    • Disease Models, Animal
    • Disease Progression
    • Gene Expression
    • Genes, ras
    • Keratin-14/genetics
    • Keratin-14/metabolism
    • Mice, Inbred BALB C
    • Mutation
    • Papilloma/genetics
    • Papilloma/metabolism
    • Skin Neoplasms/chemically induced
    • Skin Neoplasms/genetics
    • Skin Neoplasms/metabolism
    • Tetradecanoylphorbol Acetate/toxicity
    • Viral Matrix Proteins/genetics
    Strain Development
    LMP2A sequences were microinjected into (C57BL/6 X SJL) F1 single cell fertilized eggs. The K14-LMP2A transgenic animals were bred to CD-1 mice.
    Suggested Control Mice
    Wild-type littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Virology
    Donor
    Richard Longnecker, Ph.D., Northwestern University.
    Primary Reference

    Longan L, Longnecker R. Epstein-Barr virus latent membrane protein 2A has no growth-altering effects when expressed in differentiating epithelia. J Gen Virol. 2000 Sep;81(Pt 9):2245-52. (Medline PMID: 10950982)

    Shair KH, Bendt KM, Edwards RH, Nielsen JN, Moore DT, Raab-Traub N. Epstein-Barr virus-encoded latent membrane protein 1 (LMP1) and LMP2A function cooperatively to promote carcinoma development in a mouse carcinogenesis model. J Virol. 2012 May;86(9):5352-65. (Medline PMID: 22357283)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    White
    MMRRC Breeding System
    Backcross
    Breeding Scheme(s)
    • Backcross: confirm there are no reproduction issues
      • Wild-type (STRAIN) female x Heterozygous male or reciprocal mating
      • Wild-type (STRAIN) female x Hemizygous male or reciprocal mating
    • Sib-mating: confirm there are no reproduction issues
      • Wild-type female x Heterozygous male littermate or reciprocal mating
      • Wild-type female x Hemizygous male littermate or reciprocal mating
      • Heterozygous female x Heterozygous male littermate
      • Homozygous female x Homozygous male littermate
    • Intra-strain Random Mating
      • Wild-type female x Heterozygous male from the colony or reciprocal mating
      • Wild-type female x Hemizygous male from the colony or reciprocal mating
      • Heterozygous female x Heterozygous male from the colony
      • Homozygous female x Homozygous male from the colony
    Generation
    N80
    Overall Breeding Performance
    Good
    NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Undetermined Undetermined
    Homozygotes are fertile: Undetermined Undetermined
    Hetero/Hemizygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    5-9
    Recommended wean age
    4 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    041422-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    041422-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.