Strain Name:
B6;CBA-Tg(MYH6-Mb*)4Jue/Mmucd
Stock Number:
041499-UCD
Citation ID:
RRID:MMRRC_041499-UCD
Other Names:
B6;CBA-TG(HPMyo)4Jue

Strain Information

Tg(MYH6-Mb*)4Jue
Name: transgene insertion 4, Thomas Jue
Type: Allele
Species: Multi-species
Chromosome: unknown
MYH6
Name: myosin, heavy chain 6, cardiac muscle, alpha
Type: Gene
Species: Homo sapiens (human)
Chromosome: 14
Mb
Name: myoglobin
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17189
VEGA: 15
HGNC: HGNC:6915
Homologene: 3916
Genetic Alterations
The transgene included anti-sense myoglobin with a heart-specific myosin heavy chain (MHC) promoter, which targeted only heart muscle but not skeletal muscle.
Strain Development
The cryopreserved embryos (B6;CBA-TG(HpMyo)10Jue and B6;CBA-TG(HPMyo)4Jue) originated from experiments involving microinjecting anti-sense myoglobin (Mb) plasmid into oocytes in 22 September 1998. Of the 120 mice injected, 90 survived. The anti-sense plasmid had a heart-specific myosin heavy chain (MHC) promoter, which targeted only heart muscle but not skeletal muscle and provided the basis for screening the 90 survivors. Two male HP-myo1 positive animals were identified. Optical spectroscopic analysis measured the myoglobin concentrations in heart muscle found that #4 had 0.58 the wild type (WT) Mb concentration and #10 had 0.38 of the WT Mb concentration.

(Curator's Note: These development descriptions pertain to this strain as well as MMRRC:41491)
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Endocrine Deficiency
  • Hematology
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Obesity
  • Research Tools
Donor
Thomas Jue, Ph.D., University of California, Davis.
Primary Reference

unpublished

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
Backcross
Generation
N1
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041499-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.