Strain Name:
C57BL/6J-MtgxR4476Btlr/Mmmh
Stock Number:
041733-MU
Citation ID:
RRID:MMRRC_041733-MU
Other Names:
R4476 (G1), C57BL/6J-MtgxR4476Btlr
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Pcp2
Name: Purkinje cell protein 2 (L7)
Synonyms: L7, Pcp-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18545
Homologene: 7464
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Gfpt2
Name: glutamine fructose-6-phosphate transaminase 2
Synonyms: GFAT2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14584
HGNC: HGNC:4242
Homologene: 68439
Lin9
Name: lin-9 DREAM MuvB core complex component
Synonyms: 2700022J23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72568
Homologene: 35252
Parn
Name: poly(A)-specific ribonuclease (deadenylation nuclease)
Synonyms: DAN, 1200003I18Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74108
VEGA: 16
HGNC: HGNC:8609
Homologene: 31098
Acin1
Name: apoptotic chromatin condensation inducer 1
Synonyms: 2610510L13Rik, 2610036I19Rik, Acinus
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56215
Homologene: 22853
Ift122
Name: intraflagellar transport 122
Synonyms: C86139, Wdr10, sopb
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 81896
Homologene: 12819
Crispld1
Name: cysteine-rich secretory protein LCCL domain containing 1
Synonyms: Cocoacrisp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 83691
Homologene: 12857
Rab23
Name: RAB23, member RAS oncogene family
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19335
Homologene: 7503
Rab22a
Name: RAB22A, member RAS oncogene family
Synonyms: E130120E14Rik, 3732413A17Rik, Rab22
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19334
HGNC: HGNC:9764
Homologene: 10782
Exosc10
Name: exosome component 10
Synonyms: PM/Scl-100, PM-Scl, RRP6, p4, p3, p2, Pmscl2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 50912
HGNC: HGNC:9138
Homologene: 31105
Zfp667
Name: zinc finger protein 667
Synonyms: A830025F02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384763
Homologene: 23343
Sim2
Name: single-minded family bHLH transcription factor 2
Synonyms: bHLHe15
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 20465
VEGA: 16
Homologene: 3716
Kidins220
Name: kinase D-interacting substrate 220
Synonyms: 3110039L19Rik, C330002I19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 77480
VEGA: 12
Homologene: 14254
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Shn-2, Gm20114
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Me3
Name: malic enzyme 3, NADP(+)-dependent, mitochondrial
Synonyms: B230207H15Rik, 1700020C08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 109264
HGNC: HGNC:6985
Homologene: 100773
Aox1
Name: aldehyde oxidase 1
Synonyms: retinal oxidase, Aox-1, Aox-2, Aox2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11761
HGNC: HGNC:553
Homologene: 68165
Acss1
Name: acyl-CoA synthetase short-chain family member 1
Synonyms: 1110032O15Rik, AceCS2, Acas2, Acas2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68738
Homologene: 56037
Actc1
Name: actin, alpha, cardiac muscle 1
Synonyms: Actc-1, alphac-actin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11464
HGNC: HGNC:143
Homologene: 68446
Cracr2a
Name: calcium release activated channel regulator 2A
Synonyms: LOC243645, LOC381812, Efcab4b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 381812
Homologene: 41883
Neto1
Name: neuropilin (NRP) and tolloid (TLL)-like 1
Synonyms: C130005O10Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 246317
VEGA: 18
Homologene: 16367
Arsb
Name: arylsulfatase B
Synonyms: Ast-1, As-1s, Asr-1, As-1, As-1t, As-1r, As1-t, As1-r, As1, As1-s, 1110007C02Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 11881
VEGA: 13
HGNC: HGNC:714
Homologene: 73870
Ugt1a6a
Name: UDP glucuronosyltransferase 1 family, polypeptide A6A
Synonyms: UGT1.6, Ugt1a6
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 94284
Homologene: 85959
Ppfia3
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
Synonyms: 2410127E16Rik, Liprin-alpha3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76787
HGNC: HGNC:9247
Homologene: 37833
Krt90
Name: keratin 90
Synonyms: 4732456N10Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239673
Homologene: 72272
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71481
Homologene: 11849
Cntn6
Name: contactin 6
Synonyms: NB-3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 53870
HGNC: HGNC:2176
Homologene: 8702
Itgb1bp1
Name: integrin beta 1 binding protein 1
Synonyms: bodenin
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16413
Homologene: 3496
Zgpat
Name: zinc finger, CCCH-type with G patch domain
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 229007
Homologene: 41874
Or12k8
Name: olfactory receptor family 12 subfamily K member 8
Synonyms: GA_x6K02T2NLDC-33777519-33776551, MOR159-3, Olfr361
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258365
Homologene: 27142
Or13a24
Name: olfactory receptor family 13 subfamily A member 24
Synonyms: GA_x6K02T2PBJ9-42723314-42724246, MOR253-13P, MOR253-12P, MOR253-10P, MOR253-13P, MOR253-12P, Olfr1553-ps1, Olfr1523-ps1, Olfr538
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258201
Homologene: 110491
Gm14401
Name: predicted gene 14401
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 100534275
Or4g7
Name: olfactory receptor family 4 subfamily G member 7
Synonyms: GA_x6K02T2Q125-72530279-72531217, MOR245-9, Olfr1288
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258395
Homologene: 88360
A230103J11Rik
Name: RIKEN cDNA A230103J11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 320466
Sox18
Name: SRY (sex determining region Y)-box 18
Synonyms: Sry-related HMG-box gene 18, Ragl
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20672
Homologene: 7546
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 17,747,510 bp
  • A to G, chromosome 1 at 33,724,892 bp
  • A to G, chromosome 1 at 58,057,497 bp
  • TAAAAAAAAA to TAAAAAAA, chromosome 1 at 88,215,928 bp
  • T to G, chromosome 1 at 180,661,597 bp
  • G to A, chromosome 2 at 37,085,061 bp
  • A to G, chromosome 2 at 59,841,996 bp
  • T to A, chromosome 2 at 111,479,664 bp
  • T to C, chromosome 2 at 114,049,226 bp
  • G to A, chromosome 2 at 150,637,913 bp
  • C to T, chromosome 2 at 173,695,263 bp
  • C to T, chromosome 2 at 177,086,777 bp
  • T to A, chromosome 2 at 181,365,746 bp
  • T to C, chromosome 2 at 181,670,876 bp
  • T to C, chromosome 3 at 127,680,018 bp
  • G to A, chromosome 4 at 148,565,324 bp
  • A to G, chromosome 6 at 104,772,561 bp
  • T to C, chromosome 6 at 115,912,515 bp
  • T to A, chromosome 6 at 127,629,819 bp
  • A to G, chromosome 7 at 6,304,599 bp
  • G to A, chromosome 7 at 45,347,745 bp
  • T to A, chromosome 7 at 89,739,860 bp
  • T to C, chromosome 7 at 140,574,929 bp
  • T to C, chromosome 8 at 3,623,448 bp
  • A to G, chromosome 8 at 85,054,768 bp
  • C to T, chromosome 9 at 72,671,239 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • T to C, chromosome 11 at 49,824,342 bp
  • T to C, chromosome 12 at 21,270,956 bp
  • C to T, chromosome 12 at 25,011,001 bp
  • C to T, chromosome 13 at 93,807,595 bp
  • T to C, chromosome 14 at 54,645,330 bp
  • T to C, chromosome 15 at 101,557,283 bp
  • A to G, chromosome 16 at 13,664,685 bp
  • T to C, chromosome 16 at 94,125,791 bp
  • A to G, chromosome 17 at 24,576,526 bp
  • A to T, chromosome 18 at 86,404,673 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4476 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041733-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.