Strain Name:
C57BL/6J-MtgxR4502Btlr/Mmmh
Stock Number:
041754-MU
Citation ID:
RRID:MMRRC_041754-MU
Other Names:
R4502 (G1), C57BL/6J-MtgxR4502Btlr
Major Collection:

Strain Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Htr2a
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: 5-HT2A receptor, Htr-2, Htr2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 15558
VEGA: 14
HGNC: HGNC:5293
Homologene: 68073
Ldb2
Name: LIM domain binding 2
Synonyms: CLIM1, Ldb3, CLP-36, CLIM-1b, CLIM-1a
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16826
HGNC: HGNC:6533
Homologene: 989
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Mtmr7
Name: myotubularin related protein 7
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54384
HGNC: HGNC:7454
Homologene: 99732
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Usp25
Name: ubiquitin specific peptidase 25
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 30940
VEGA: 16
Homologene: 8374
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: 1200015F06Rik, Mypt1, D10Ertd625e, 5730577I22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Slc49a4
Name: solute carrier family 49 member 4
Synonyms: RCC4, Dirc2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224132
Homologene: 13137
Cic
Name: capicua transcriptional repressor
Synonyms: 1200010B10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71722
Homologene: 87637
Cand1
Name: cullin associated and neddylation disassociated 1
Synonyms: 6330512O03Rik, 2310038O07Rik, D10Ertd516e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71902
Homologene: 10202
Tmem131
Name: transmembrane protein 131
Synonyms: CC28, 2610524E03Rik, YR-23, D1Bwg0491e, Neg, Rw1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 56030
Homologene: 32428
Kank4
Name: KN motif and ankyrin repeat domains 4
Synonyms: Ankrd38
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242553
Homologene: 18244
Chodl
Name: chondrolectin
Synonyms: MT75, 3110074E07Rik, PRED12
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 246048
Homologene: 11795
Pi4kb
Name: phosphatidylinositol 4-kinase beta
Synonyms: ESTM41, Pik4cb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 107650
HGNC: HGNC:8984
Homologene: 6741
Scpep1
Name: serine carboxypeptidase 1
Synonyms: 2410018F01Rik, Risc, 4833411K15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74617
Homologene: 41443
Ccdc171
Name: coiled-coil domain containing 171
Synonyms: 4930418J05Rik, A330015D16Rik, 4930473A06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320226
Homologene: 27942
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 329002
Homologene: 7198
Sil1
Name: SIL1 nucleotide exchange factor
Synonyms: 1810057E01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 81500
VEGA: 18
Homologene: 32544
Eea1
Name: early endosome antigen 1
Synonyms: B230358H09Rik, ZFYVE2, A430109M19Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216238
VEGA: 10
HGNC: HGNC:3185
Homologene: 37822
Tnks1bp1
Name: tankyrase 1 binding protein 1
Synonyms: TAB182
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228140
Homologene: 14117
Cep295
Name: centrosomal protein 295
Synonyms: LOC382128, 5830418K08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319675
Homologene: 27936
Rpl5
Name: ribosomal protein L5
Synonyms: U21RNA, Ska23, Skam23Jus, Skax23
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100503670
Homologene: 110649
Klhl1
Name: kelch-like 1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 93688
HGNC: HGNC:6352
Homologene: 56903
Dpyd
Name: dihydropyrimidine dehydrogenase
Synonyms: DPD, E330028L06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99586
HGNC: HGNC:3012
Homologene: 85
Ulk3
Name: unc-51-like kinase 3
Synonyms: 1200015E14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71742
VEGA: 9
Homologene: 68482
Hc
Name: hemolytic complement
Synonyms: C5a, C5, He, Hfib2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15139
HGNC: HGNC:1331
Homologene: 20412
Ppargc1b
Name: peroxisome proliferative activated receptor, gamma, coactivator 1 beta
Synonyms: PGC-1beta/ERRL1, 4631412G21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 170826
Homologene: 15776
Tdrd5
Name: tudor domain containing 5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 214575
Homologene: 18312
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: 4632428N09Rik, Aof1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218214
Homologene: 14695
Vmn2r80
Name: vomeronasal 2, receptor 80
Synonyms: EG624765
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 624765
Homologene: 83483
Gpr39
Name: G protein-coupled receptor 39
Synonyms: 4933415E13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71111
HGNC: HGNC:4496
Homologene: 20380
Arf5
Name: ADP-ribosylation factor 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11844
HGNC: HGNC:658
Homologene: 129625
Plch2
Name: phospholipase C, eta 2
Synonyms: PLCeta2, A930027K05Rik, Plcl4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269615
Homologene: 85172
Slc2a9
Name: solute carrier family 2 (facilitated glucose transporter), member 9
Synonyms: Glut9, SLC2A9B, SLC2a9A
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 117591
Homologene: 69290
Agtr1b
Name: angiotensin II receptor, type 1b
Synonyms: Agtr-1b, AT1B, Angtr-1b
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11608
HGNC: HGNC:336
Homologene: 84871
Kcnt2
Name: potassium channel, subfamily T, member 2
Synonyms: E330038N15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240776
Homologene: 16121
Vps33b
Name: vacuolar protein sorting 33B
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233405
Homologene: 10261
G930045G22Rik
Name: RIKEN cDNA G930045G22 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Or2p2
Name: olfactory receptor family 2 subfamily P member 2
Synonyms: GA_x6K02T2QHY8-12181473-12182423, MOR256-14, Olfr1370
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 258528
Homologene: 105231
Ldhb
Name: lactate dehydrogenase B
Synonyms: lactate dehydrogenase-B, Ldh-2, H-Ldh
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16832
HGNC: HGNC:6541
Homologene: 55647
Zfp938
Name: zinc finger protein 938
Synonyms: B230315N10Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237411
Homologene: 135924
Rbbp8nl
Name: RBBP8 N-terminal like
Synonyms: BC066135
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 271887
Homologene: 76811
Tdrd9
Name: tudor domain containing 9
Synonyms: 4930441E05Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 74691
Homologene: 14311
Bmp8a
Name: bone morphogenetic protein 8a
Synonyms: osteogenic protein 2, OP2, Bmp7r1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12163
Homologene: 135983
Or6aa1
Name: olfactory receptor family 6 subfamily AA member 1
Synonyms: GA_x6K02T2NHDJ-9712819-9713778, MOR104-2, Olfr303
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258612
Homologene: 105157
Or5k15
Name: olfactory receptor family 5 subfamily J member 15
Synonyms: GA_x54KRFPKG5P-55108059-55107100, MOR184-6, Olfr178
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258999
Homologene: 79412
Atp6v0d1
Name: ATPase, H+ transporting, lysosomal V0 subunit D1
Synonyms: Ac39, Atp6d, P39, Vma6, lysosomal 38kDa
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11972
Homologene: 3444
Wnt9a
Name: wingless-type MMTV integration site family, member 9A
Synonyms: Wnt14
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216795
Homologene: 20722
Zfp689
Name: zinc finger protein 689
Synonyms: 4933416E05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71131
Homologene: 16318
Gm10550
Name: predicted gene 10550
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 34,247,691 bp
  • T to C, chromosome 1 at 36,825,479 bp
  • T to C, chromosome 1 at 45,348,677 bp
  • T to C, chromosome 1 at 93,750,987 bp
  • G to A, chromosome 1 at 125,677,991 bp
  • G to T, chromosome 1 at 140,507,747 bp
  • T to A, chromosome 1 at 156,300,764 bp
  • T to C, chromosome 2 at 35,006,252 bp
  • C to T, chromosome 2 at 85,062,647 bp
  • T to A, chromosome 2 at 125,226,044 bp
  • G to T, chromosome 2 at 180,279,196 bp
  • A to C, chromosome 3 at 20,315,798 bp
  • T to A, chromosome 3 at 94,996,607 bp
  • G to T, chromosome 3 at 118,797,537 bp
  • A to G, chromosome 4 at 83,864,323 bp
  • G to A, chromosome 4 at 98,777,098 bp
  • T to A, chromosome 4 at 123,342,399 bp
  • T to C, chromosome 4 at 154,985,441 bp
  • T to C, chromosome 5 at 38,398,811 bp
  • T to C, chromosome 5 at 44,669,407 bp
  • T to C, chromosome 5 at 73,088,397 bp
  • T to C, chromosome 5 at 107,904,857 bp
  • T to C, chromosome 6 at 28,425,776 bp
  • T to C, chromosome 6 at 50,846,665 bp
  • T to C, chromosome 6 at 142,490,457 bp
  • C to T, chromosome 7 at 25,288,467 bp
  • G to A, chromosome 7 at 80,287,907 bp
  • T to A, chromosome 7 at 86,395,277 bp
  • C to A, chromosome 7 at 127,448,753 bp
  • T to C, chromosome 8 at 40,558,162 bp
  • A to G, chromosome 8 at 105,565,798 bp
  • A to G, chromosome 9 at 15,317,661 bp
  • A to G, chromosome 9 at 53,495,946 bp
  • A to G, chromosome 9 at 57,593,229 bp
  • A to T, chromosome 10 at 79,148,930 bp
  • A to G, chromosome 10 at 82,226,271 bp
  • G to A, chromosome 10 at 96,039,565 bp
  • G to T, chromosome 10 at 108,249,478 bp
  • T to C, chromosome 10 at 119,216,667 bp
  • T to C, chromosome 11 at 59,328,537 bp
  • T to C, chromosome 11 at 88,944,385 bp
  • T to C, chromosome 12 at 111,993,825 bp
  • A to T, chromosome 13 at 21,072,746 bp
  • C to T, chromosome 13 at 47,063,077 bp
  • T to A, chromosome 14 at 74,641,988 bp
  • A to G, chromosome 14 at 96,517,846 bp
  • T to C, chromosome 16 at 35,719,417 bp
  • T to C, chromosome 16 at 58,890,176 bp
  • T to C, chromosome 16 at 77,115,396 bp
  • A to G, chromosome 16 at 78,931,444 bp
  • T to C, chromosome 18 at 35,317,875 bp
  • T to C, chromosome 18 at 61,302,679 bp
  • T to C, chromosome 18 at 82,636,954 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4502 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041754-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.