Strain Name:
B6J.129S6(SJL)-Gt(ROSA)26Sortm12.1Mgn/Mmmh
Stock Number:
042204-MU
Citation ID:
RRID:MMRRC_042204-MU
Other Names:
Rosa26(3TF.mCherry)Mgn, B6J.129S6-Gt(ROSA)26Sortm1(Pdx1,MafA,Neurog3.mCherry)Mgn/Mmmh

Strain Information

Gt(ROSA)26Sortm12.1Mgn
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 12.1, Mark A Magnusson
Synonyms: Rosa26(3TF.mCherry)Mgn, Rosa263TF.mCherry, Rs263TF.mCherry, Gt(ROSA)26Sortm1(Pdx1,MafA,Neurog3.mCherry)Mgn
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: beta geo, R26, Gtrosa26, Thumpd3as1, Gtrgeo26, ROSA26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Targeted Mutation
NCBI: 14910
Genetic Alterations
This mouse line contains a bi-directional gene inserted into a modified Rosa26 locus that drives expression or mCherry and three transcription factors (MafA, Neurog3, and Pdx1)
Genotype Determination
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac
Phenotype

Homozygous: Lethal

Heterozygous: While mice are fertile and viable, there is a reduced number of transgenic mice born than expected according to Mendelian genetics (25% of pups vs 50% expected)

MeSH Terms
  • Acinar Cells/drug effects
  • Acinar Cells/pathology
  • Adenoviridae/metabolism
  • Alleles
  • Animals
  • Cellular Reprogramming/drug effects
  • Diabetes Mellitus, Experimental/pathology
  • Doxycycline/pharmacology
  • Gene Expression Profiling
  • Homeodomain Proteins/metabolism
  • Immunity
  • Inflammation/pathology
  • Insulin-Secreting Cells/drug effects
  • Insulin-Secreting Cells/pathology
  • Macrophages/drug effects
  • Macrophages/pathology
  • Metaplasia
  • Mice, Transgenic
  • Organ Size/drug effects
  • Pancreas/pathology
  • Pancreatic Ducts/pathology
  • Reproducibility of Results
  • Transcription Factors/metabolism
  • Transgenes
Strain Development
This allele was derived by recombinase-mediated cassette exchange (RMCE) using mouse ES cells (mESC) that had been previously engineered to contain a loxed cassette acceptor allele. The exchange vector for the allele was made by cloning both the mCherry DNA sequences and a 2A peptide-based expression cassette containing sequences for Neurog3, Pdx1, and MafA into separate sites of a pTRE-Tight-BI vector. The bi-Tet operator assemblage was then inserted between the Lox71 and Lox2272 sites in pMCS.71/2272.Hygro. The construct was electroporated into 129S6/SvEvTac derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to B6;SJL-Tg(ACTFLPe)9205Dym/J to remove the hygromycin cassette. Mice were then backcrossed to C57BL/6J for >10 generations.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cell Biology
  • Diabetes
Donor
Mark Magnuson, M.D., Vanderbilt University.
Primary Reference

Clayton HW, Osipovich AB, Stancill JS, Schneider JD, Vianna PG, Shanks CM,Yuan W, Gu G, Manduchi E, Stoeckert CJ Jr, Magnuson MA. Pancreatic InflammationRedirects Acinar to β Cell Reprogramming. Cell Rep. 2016 Nov 15;17(8):2028-2041. doi: 10.1016/j.celrep.2016.10.068. (Medline PMID: 27851966)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N10+ (C57BL/6J)
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 6 to 8 months
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3-4 weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042204-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.