Strain Name:
C57BL/6J-MtgxR5298Btlr/Mmmh
Stock Number:
042881-MU
Citation ID:
RRID:MMRRC_042881-MU
Other Names:
R5298 (G1), C57BL/6J-MtgxR5298Btlr
Major Collection:

Strain Information

Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Prkca
Name: protein kinase C, alpha
Synonyms: Pkca
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18750
HGNC: HGNC:9393
Homologene: 55679
Csde1
Name: cold shock domain containing E1, RNA binding
Synonyms: unr, D3Jfr1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229663
Homologene: 5179
Mon2
Name: MON2 homolog, regulator of endosome to Golgi trafficking
Synonyms: SF21, 2610528O22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67074
VEGA: 10
Homologene: 44309
Plcg2
Name: phospholipase C, gamma 2
Synonyms: Plcg-2, PLCgamma2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234779
HGNC: HGNC:9066
Homologene: 55671
Parg
Name: poly (ADP-ribose) glycohydrolase
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 26430
HGNC: HGNC:8605
Homologene: 50532
Tasor2
Name: transcription activation suppressor family member 2
Synonyms: BC016423, Fam208b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105203
VEGA: 13
Homologene: 26435
Ehmt2
Name: euchromatic histone lysine N-methyltransferase 2
Synonyms: NG36, G9a, D17Ertd710e, Bat8, KMT1C
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 110147
Homologene: 48460
Lef1
Name: lymphoid enhancer binding factor 1
Synonyms: lymphoid enhancer factor 1, Lef-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16842
HGNC: HGNC:6551
Homologene: 7813
Spag9
Name: sperm associated antigen 9
Synonyms: 4733401I23Rik, syd1, 4831406C20Rik, Mapk8ip4, JIP4, 3110018C07Rik, JLP
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 70834
Homologene: 2954
Tmem201
Name: transmembrane protein 201
Synonyms: D4Ertd429e, Samp1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230917
Homologene: 35319
Zfp507
Name: zinc finger protein 507
Synonyms: A230056M16Rik, 1810022O10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 668501
Homologene: 8942
Gle1
Name: GLE1 RNA export mediator
Synonyms: 4933405K21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74412
HGNC: HGNC:4315
Homologene: 20379
Ackr4
Name: atypical chemokine receptor 4
Synonyms: VSHK1, CCR11, CCBP2, PPR1, CCX-CKR, A630091E18Rik, Ccrl1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 252837
HGNC: HGNC:1611
Homologene: 49474
Scn4a
Name: sodium channel, voltage-gated, type IV, alpha
Synonyms: Nav1.4, mH2, SkM1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 110880
Homologene: 283
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, 2900022L05Rik, Dnhd3, Dnahc2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Pkhd1l1
Name: polycystic kidney and hepatic disease 1-like 1
Synonyms: D86 mRNA, PKHDL1, fibrocystin L
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 192190
Homologene: 16332
Magi2
Name: membrane associated guanylate kinase, WW and PDZ domain containing 2
Synonyms: S-SCAM, Acvrinp1, Magi-2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 50791
Homologene: 8189
Trim45
Name: tripartite motif-containing 45
Synonyms: 4921530N01Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229644
Homologene: 11865
Ccdc97
Name: coiled-coil domain containing 97
Synonyms: 2810446P04Rik, 1200014H14Rik, D7Ertd462e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 52132
Homologene: 32701
Or5p81
Name: olfactory receptor family 5 subfamily P member 81
Synonyms: GA_x6K02T2PBJ9-10997715-10998659, MOR204-34, Olfr510
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258308
Hadhb
Name: hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta
Synonyms: 4930479F15Rik, Mtpb
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231086
HGNC: HGNC:4803
Homologene: 153
Celsr3
Name: cadherin, EGF LAG seven-pass G-type receptor 3
Synonyms: Fmi1, flamingo, Adgrc3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 107934
HGNC: HGNC:3230
Homologene: 1077
Per3
Name: period circadian clock 3
Synonyms: mPer3, 2810049O06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18628
HGNC: HGNC:8847
Homologene: 7886
Cdcp2
Name: CUB domain containing protein 2
Synonyms: D030010E02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242603
Homologene: 138406
Vmn1r193
Name: vomeronasal 1 receptor 193
Synonyms: V1ri9
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 171259
Homologene: 110880
Adat2
Name: adenosine deaminase, tRNA-specific 2
Synonyms: 4933426M09Rik, Deadc1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66757
Homologene: 6262
Lrriq4
Name: leucine-rich repeats and IQ motif containing 4
Synonyms: 4930558O21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68307
Homologene: 12260
Klhl18
Name: kelch-like 18
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270201
Homologene: 14805
Reep4
Name: receptor accessory protein 4
Synonyms: 2700029E10Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 72549
VEGA: 14
Homologene: 11888
Clip4
Name: CAP-GLY domain containing linker protein family, member 4
Synonyms: 1700074B05Rik, 5830409B12Rik, 4833417L20Rik, 1700024K14Rik, Rsnl2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 78785
VEGA: 17
Homologene: 11662
Qsox2
Name: quiescin Q6 sulfhydryl oxidase 2
Synonyms: QSOX2, Qscn6l1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227638
Homologene: 65608
Fuca1
Name: fucosidase, alpha-L- 1, tissue
Synonyms: Afuc, 0610006A03Rik, 9530055J05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 71665
HGNC: HGNC:4006
Homologene: 20078
Or4c127
Name: olfactory receptor family 4 subfamily C member 127
Synonyms: GA_x6K02T2Q125-51434523-51435437, MOR234-1, Olfr1262
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258976
Homologene: 74064
Sowaha
Name: sosondowah ankyrin repeat domain family member A
Synonyms: A830006N08Rik, Ankrd43
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237761
Homologene: 18488
D330020A13Rik
Name: RIKEN cDNA D330020A13 gene
Synonyms: ENSMUSG00000053059
Type: Gene
Species: Mouse
Chromosome: 6
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 118,547,920 bp
  • A to C, chromosome 2 at 26,214,062 bp
  • C to G, chromosome 2 at 29,948,943 bp
  • T to A, chromosome 2 at 90,002,460 bp
  • G to T, chromosome 3 at 30,645,332 bp
  • A to G, chromosome 3 at 100,925,471 bp
  • T to A, chromosome 3 at 103,047,209 bp
  • A to T, chromosome 3 at 131,194,667 bp
  • T to C, chromosome 4 at 107,106,985 bp
  • C to A, chromosome 4 at 135,936,926 bp
  • A to G, chromosome 4 at 149,727,138 bp
  • A to T, chromosome 4 at 151,029,209 bp
  • T to C, chromosome 5 at 20,569,162 bp
  • T to C, chromosome 5 at 30,177,011 bp
  • T to C, chromosome 6 at 120,294,816 bp
  • A to G, chromosome 7 at 25,716,007 bp
  • T to C, chromosome 7 at 35,775,996 bp
  • A to G, chromosome 7 at 108,668,072 bp
  • A to G, chromosome 8 at 117,605,249 bp
  • A to G, chromosome 9 at 104,098,887 bp
  • A to G, chromosome 9 at 108,842,931 bp
  • G to C, chromosome 9 at 110,436,127 bp
  • A to G, chromosome 10 at 13,556,906 bp
  • A to G, chromosome 10 at 92,921,348 bp
  • A to G, chromosome 10 at 123,010,606 bp
  • C to T, chromosome 11 at 53,479,528 bp
  • C to T, chromosome 11 at 69,458,920 bp
  • T to C, chromosome 11 at 94,100,135 bp
  • T to C, chromosome 11 at 106,339,386 bp
  • A to T, chromosome 11 at 108,012,684 bp
  • T to C, chromosome 13 at 3,595,613 bp
  • A to T, chromosome 13 at 22,219,555 bp
  • C to A, chromosome 14 at 32,202,253 bp
  • G to A, chromosome 14 at 70,548,197 bp
  • A to T, chromosome 15 at 44,504,046 bp
  • C to T, chromosome 17 at 34,899,091 bp
  • A to G, chromosome 17 at 71,834,225 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5298 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042881-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.