Strain Name:
C57BL/6J-MtgxR5414Btlr/Mmmh
Stock Number:
042983-MU
Citation ID:
RRID:MMRRC_042983-MU
Other Names:
R5414 (G1), C57BL/6J-MtgxR5414Btlr
Major Collection:

Strain Information

Xrcc1
Name: X-ray repair complementing defective repair in Chinese hamster cells 1
Synonyms: Xrcc-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22594
Homologene: 31368
Myo1e
Name: myosin IE
Synonyms: 9130023P14Rik, 2310020N23Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71602
VEGA: 9
HGNC: HGNC:7599
Homologene: 55864
Bcat1
Name: branched chain aminotransferase 1, cytosolic
Synonyms: BCATc, Bcat-1, Eca39
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12035
HGNC: HGNC:976
Homologene: 20320
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Phr1, C130061D10Rik, Pam
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Washc4
Name: WASH complex subunit 4
Synonyms: A230046K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 319277
VEGA: 10
Homologene: 123926
Ppp6r3
Name: protein phosphatase 6, regulatory subunit 3
Synonyms: Pptcs3, Pp6r3, 4930528G08Rik, Saps3, D19Bwg1430e, D19Ertd703e, 9130026N02Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 52036
VEGA: 19
HGNC: HGNC:1173
Homologene: 115911
Atr
Name: ataxia telangiectasia and Rad3 related
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245000
HGNC: HGNC:882
Homologene: 96916
Nploc4
Name: NPL4 homolog, ubiquitin recognition factor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217365
Homologene: 5403
Focad
Name: focadhesin
Synonyms: BC057079
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230393
Homologene: 9842
Coro1c
Name: coronin, actin binding protein 1C
Synonyms: CRN2, coronin 3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 23790
HGNC: HGNC:2254
Homologene: 56537
Thra
Name: thyroid hormone receptor alpha
Synonyms: Thra2, T3Ralpha, TR alpha 2, Thra1, Rvr, Erba, TR alpha 1, 6430529J03Rik, c-erbAalpha, Nr1a1, T3R[a]
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21833
Homologene: 37747
Cdan1
Name: codanin 1
Synonyms: CDA-I, 1500015A01Rik, codanin-1, CDA1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68968
HGNC: HGNC:1713
Homologene: 16324
Cnih1
Name: cornichon family AMPA receptor auxiliary protein 1
Synonyms: Cnih
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12793
Homologene: 4219
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: 2610100B16Rik, Odz3, Ten-m3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23965
Homologene: 22673
Ibtk
Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase
Synonyms: 5430411K16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108837
Homologene: 34661
Pgs1
Name: phosphatidylglycerophosphate synthase 1
Synonyms: 2610019F11Rik, 4933424M23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74451
Homologene: 5467
Scamp5
Name: secretory carrier membrane protein 5
Synonyms: Sc5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56807
VEGA: 9
Homologene: 56880
Ddx11
Name: DEAD/H box helicase 11
Synonyms: 4732462I11Rik, CHLR1, essa15a, KRG2, CHL1, DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 320209
VEGA: 17
Homologene: 68973
Cd34
Name: CD34 antigen
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12490
HGNC: HGNC:1662
Homologene: 1343
Atp2b2
Name: ATPase, Ca++ transporting, plasma membrane 2
Synonyms: jog, PMCA2, wms, Tmy, D6Abb2e, Gena300
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11941
HGNC: HGNC:815
Homologene: 56150
Akr1c18
Name: aldo-keto reductase family 1, member C18
Synonyms: 20alpha-HSD, 20alpha-hydroxysteroid dehydrogenase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105349
HGNC: HGNC:386
Homologene: 128661
Gcgr
Name: glucagon receptor
Synonyms: GR
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14527
HGNC: HGNC:4192
Homologene: 131
C1rl
Name: complement component 1, r subcomponent-like
Synonyms: C1rl1, C1r-LP
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232371
Homologene: 9559
Pdk4
Name: pyruvate dehydrogenase kinase, isoenzyme 4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 27273
HGNC: HGNC:8812
Homologene: 129720
Pgm5
Name: phosphoglucomutase 5
Synonyms: aciculin, 9530034F03Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226041
HGNC: HGNC:8908
Homologene: 74881
Svep1
Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Synonyms: 4833413O10Rik, D430029O09Rik, 1110021D17Rik, Polydom
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 64817
Homologene: 23386
Sis
Name: sucrase isomaltase
Synonyms: sucrase-isomaltase, 2010204N08Rik, Si-s
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69983
Homologene: 37424
Zfp629
Name: zinc finger protein 629
Synonyms: 9330199A09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 320683
Homologene: 65318
Fbn2
Name: fibrillin 2
Synonyms: Fib-2, sy, Sne
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Vmn2r3
Name: vomeronasal 2, receptor 3
Synonyms: EG637004
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 637004
Hfm1
Name: HFM1, ATP-dependent DNA helicase homolog
Synonyms: Mer3, LOC381663, Sec63d1, A330009G12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 330149
Homologene: 87103
Kcnh4
Name: potassium voltage-gated channel, subfamily H (eag-related), member 4
Synonyms: BEC2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380728
HGNC: HGNC:6253
Homologene: 8180
Galnt16
Name: polypeptide N-acetylgalactosaminyltransferase 16
Synonyms: Galntl1, 5730405L21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 108760
VEGA: 12
Homologene: 18907
Trim30a
Name: tripartite motif-containing 30A
Synonyms: Trim30, Rpt-1, Rpt1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20128
Homologene: 114426
Ros1
Name: Ros1 proto-oncogene
Synonyms: Ros-1, c-ros
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19886
Homologene: 2207
Nos1ap
Name: nitric oxide synthase 1 (neuronal) adaptor protein
Synonyms: 6330408P19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70729
Homologene: 135990
Abca5
Name: ATP-binding cassette, sub-family A member 5
Synonyms: B930033A02Rik, ABC13
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217265
HGNC: HGNC:35
Homologene: 10263
Rin3
Name: Ras and Rab interactor 3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217835
Homologene: 11748
Pdzrn3
Name: PDZ domain containing RING finger 3
Synonyms: Semcap3, LNX3, 1110020C07Rik, semaphorin cytoplasmic domain-associated protein 3A
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 55983
Homologene: 10328
Celsr3
Name: cadherin, EGF LAG seven-pass G-type receptor 3
Synonyms: Adgrc3, flamingo, Fmi1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 107934
HGNC: HGNC:3230
Homologene: 1077
Akt3
Name: thymoma viral proto-oncogene 3
Synonyms: Nmf350, D930002M15Rik, PKB gamma
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23797
HGNC: HGNC:393
Homologene: 55904
Gm6327
Name: predicted gene 6327
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Gm10288
Name: predicted gene 10288
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 100040540
Acan
Name: aggrecan
Synonyms: b2b183Clo, Agc1, Cspg1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11595
HGNC: HGNC:319
Homologene: 137204
Clec2h
Name: C-type lectin domain family 2, member h
Synonyms: Clrf
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 94071
Homologene: 131164
Or4b12
Name: olfactory receptor family 4 subfamily B member 12
Synonyms: MOR227-5, Olfr1271, GA_x6K02T2Q125-51620802-51619885
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258789
HGNC: HGNC:8290
Prag1
Name: PEAK1 related kinase activating pseudokinase 1
Synonyms: D8Ertd82e, NACK
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244418
Homologene: 18256
Gbp2b
Name: guanylate binding protein 2b
Synonyms: Gbp1, Gbp-1, Mag-1, Mpa1, Mpa-1, LIMIT
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14468
Homologene: 137233
Ifit1bl1
Name: interferon induced protein with tetratricpeptide repeats 1B like 1
Synonyms: Gm14446
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 667373
Homologene: 78213
Gm10192
Name: predicted gene 10192
Type: Gene
Species: Mouse
Chromosome: 4
Krt33b
Name: keratin 33B
Synonyms: Ha4, Krt1-3, Ha3, mHa3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16671
Homologene: 74433
Tcstv2b
Name: 2 cell stage variable group member 2B
Synonyms: Gm21761
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 106029237
Gm17106
Name: predicted gene 17106
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Gm4181
Name: predicted gene 4181
Type: Gene
Species: Mouse
Chromosome: 14
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 170,349,399 bp
  • A to G, chromosome 1 at 177,050,251 bp
  • A to G, chromosome 1 at 194,947,911 bp
  • T to C, chromosome 2 at 90,265,702 bp
  • A to G, chromosome 2 at 120,729,584 bp
  • T to A, chromosome 3 at 64,259,557 bp
  • C to A, chromosome 3 at 72,952,493 bp
  • T to C, chromosome 3 at 142,599,091 bp
  • T to C, chromosome 3 at 146,838,962 bp
  • T to C, chromosome 4 at 58,206,322 bp
  • C to T, chromosome 4 at 88,410,702 bp
  • T to C, chromosome 4 at 97,183,109 bp
  • A to G, chromosome 5 at 106,902,076 bp
  • A to G, chromosome 5 at 113,848,546 bp
  • T to C, chromosome 6 at 5,485,499 bp
  • C to T, chromosome 6 at 101,153,311 bp
  • G to A, chromosome 6 at 113,842,141 bp
  • C to T, chromosome 6 at 124,508,468 bp
  • G to T, chromosome 6 at 128,674,786 bp
  • A to G, chromosome 6 at 145,015,447 bp
  • A to G, chromosome 7 at 24,570,218 bp
  • A to G, chromosome 7 at 79,100,988 bp
  • A to C, chromosome 7 at 104,411,141 bp
  • T to C, chromosome 7 at 127,611,282 bp
  • C to G, chromosome 8 at 36,139,622 bp
  • A to C, chromosome 8 at 48,236,355 bp
  • A to G, chromosome 9 at 57,447,224 bp
  • C to A, chromosome 9 at 70,322,358 bp
  • T to C, chromosome 9 at 85,726,689 bp
  • A to G, chromosome 9 at 95,870,704 bp
  • A to G, chromosome 9 at 108,840,042 bp
  • T to C, chromosome 10 at 52,155,093 bp
  • A to G, chromosome 10 at 83,556,103 bp
  • A to G, chromosome 11 at 98,760,957 bp
  • T to C, chromosome 11 at 100,029,786 bp
  • T to C, chromosome 11 at 100,746,896 bp
  • A to G, chromosome 11 at 110,314,622 bp
  • T to G, chromosome 11 at 118,014,676 bp
  • A to T, chromosome 11 at 120,413,643 bp
  • C to T, chromosome 11 at 120,536,082 bp
  • A to T, chromosome 12 at 80,584,048 bp
  • C to T, chromosome 12 at 102,389,857 bp
  • T to A, chromosome 13 at 4,136,735 bp
  • A to C, chromosome 13 at 119,912,336 bp
  • T to C, chromosome 14 at 46,778,983 bp
  • C to T, chromosome 14 at 51,635,590 bp
  • A to T, chromosome 14 at 103,306,261 bp
  • T to A, chromosome 16 at 12,760,358 bp
  • T to A, chromosome 16 at 32,985,965 bp
  • A to G, chromosome 17 at 66,148,768 bp
  • C to A, chromosome 18 at 58,093,405 bp
  • A to G, chromosome 19 at 3,507,330 bp
  • A to G, chromosome 19 at 24,709,325 bp
  • T to A, chromosome 19 at 34,593,924 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5414 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042983-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.