Strain Name:
C57BL/6J-MtgxR5487Btlr/Mmmh
Stock Number:
043048-MU
Citation ID:
RRID:MMRRC_043048-MU
Other Names:
R5487 (G1), C57BL/6J-MtgxR5487Btlr
Major Collection:

Strain Information

Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: LUCA-1, SemA, sema5, Semaa, semaV, SemA
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20347
Homologene: 20972
Lcat
Name: lecithin cholesterol acyltransferase
Synonyms: D8Wsu61e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16816
HGNC: HGNC:6522
Homologene: 68042
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: D5Ertd66e, Bwf1, 2610509D04Rik, Bchs, Ggtb3, Alfy
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72145
Homologene: 22855
Atp2a2
Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Synonyms: D5Wsu150e, Serca2a, SERCA2, 9530097L16Rik, SERCA2B, sarco/endoplasmic reticulum Ca2+-ATPase 2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11938
HGNC: HGNC:812
Homologene: 80167
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, ALL-1, Mll1, Cxxc7, Mll, HTRX1, All1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: 8030453L17Rik, KMT2H, chromatin remodeling factor, E430018P19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192195
Homologene: 10225
Ecpas
Name: Ecm29 proteasome adaptor and scaffold
Synonyms: AI314180
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230249
Homologene: 6056
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
Camta1
Name: calmodulin binding transcription activator 1
Synonyms: 1810059M14Rik, 2310058O09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100072
Homologene: 18942
Uty
Name: ubiquitously transcribed tetratricopeptide repeat containing, Y-linked
Synonyms: Hydb
Type: Gene
Species: Mouse
Chromosome: Y
NCBI: 22290
Tph2
Name: tryptophan hydroxylase 2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216343
Homologene: 27831
Prkcb
Name: protein kinase C, beta
Synonyms: Prkcb2, PKC-Beta, A130082F03Rik, Prkcb1, Pkcb
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18751
HGNC: HGNC:9395
Homologene: 56424
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Zfp750
Name: zinc finger protein 750
Synonyms: A030007D23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 319530
Homologene: 36423
G6pc2
Name: glucose-6-phosphatase, catalytic, 2
Synonyms: IGRP, islet specific glucose-6-phosphatase, G6pc-rs
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14378
Homologene: 41423
Fbxo24
Name: F-box protein 24
Synonyms: Fbx24, 4933422D21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71176
Homologene: 14131
Otog
Name: otogelin
Synonyms: Otgn
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18419
HGNC: HGNC:8516
Homologene: 8421
Abca8b
Name: ATP-binding cassette, sub-family A member 8b
Synonyms: Abca8
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Krba1
Name: KRAB-A domain containing 1
Synonyms: A930040G15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 77827
Homologene: 15880
Fam237b
Name: family with sequence similarity 237, member B
Synonyms: Gm8773, Gm29287
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 667705
Mical2
Name: microtubule associated monooxygenase, calponin and LIM domain containing 2
Synonyms: Micalcl, Ebitein1, 4921517J23Rik, 5330438E18Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 320878
Homologene: 8760
Serpinf2
Name: serine (or cysteine) peptidase inhibitor, clade F, member 2
Synonyms: alpha 2 antiplasmin, Pli
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18816
HGNC: HGNC:9075
Homologene: 719
Bap1
Name: Brca1 associated protein 1
Synonyms: 2300006C11Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 104416
HGNC: HGNC:950
Homologene: 3421
Ankzf1
Name: ankyrin repeat and zinc finger domain containing 1
Synonyms: D1Ertd161e, 2810025E10Rik, 1300008P06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 52231
Homologene: 41225
Cd46
Name: CD46 antigen, complement regulatory protein
Synonyms: CD46, Mcp
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17221
HGNC: HGNC:6953
Homologene: 7832
Noxo1
Name: NADPH oxidase organizer 1
Synonyms: 2310034C04Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 71893
Homologene: 12418
Vmn2r65
Name: vomeronasal 2, receptor 65
Synonyms: ENSMUSG00000070600
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100009609
Lrriq4
Name: leucine-rich repeats and IQ motif containing 4
Synonyms: 4930558O21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68307
Homologene: 12260
Agbl5
Name: ATP/GTP binding protein-like 5
Synonyms: 9430057O19Rik, Ccp5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231093
Homologene: 11053
Ccdc85a
Name: coiled-coil domain containing 85A
Synonyms: E030025D05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216613
Homologene: 65605
Gm4871
Name: predicted gene 4871
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231885
Homologene: 86127
Dnajb12
Name: DnaJ heat shock protein family (Hsp40) member B12
Synonyms: mDj10
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 56709
Homologene: 9749
Cblc
Name: Casitas B-lineage lymphoma c
Synonyms: 2310079L19Rik, 2310076I21Rik, Cbl3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80794
Homologene: 8108
Oas1a
Name: 2'-5' oligoadenylate synthetase 1A
Synonyms: L3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 246730
HGNC: HGNC:8086
Homologene: 1903
Pou5f2
Name: POU domain class 5, transcription factor 2
Synonyms: Sprm1, 1700013G10Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75507
Homologene: 83454
Ccdc122
Name: coiled-coil domain containing 122
Synonyms: 4933415L06Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 108811
Homologene: 51651
Erich4
Name: glutamate rich 4
Synonyms: Gm7092
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 632778
Homologene: 86877
Alg3
Name: ALG3 alpha-1,3- mannosyltransferase
Synonyms: D16Ertd36e
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208624
Homologene: 4228
Tnni3
Name: troponin I, cardiac 3
Synonyms: cardiac troponin I, cTnI, Tn1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21954
Homologene: 309
Pcdha9
Name: protocadherin alpha 9
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 192161
HGNC: HGNC:8664
Homologene: 130700
Igdcc3
Name: immunoglobulin superfamily, DCC subclass, member 3
Synonyms: 2810401C09Rik, WI-14920, Punc
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19289
VEGA: 9
HGNC: HGNC:9700
Homologene: 3590
Fzd4
Name: frizzled class receptor 4
Synonyms: Fz4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14366
HGNC: HGNC:4042
Homologene: 7325
Xntrpc
Name: Xndc1-transient receptor potential cation channel, subfamily C, member 2 readthrough
Synonyms: Xndr-trpc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102443351
Homologene: 134407
Gm10663
Name: predicted gene 10663
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 75,192,664 bp
  • T to G, chromosome 1 at 195,068,170 bp
  • T to C, chromosome 2 at 69,226,577 bp
  • C to T, chromosome 2 at 76,812,552 bp
  • C to A, chromosome 3 at 30,659,995 bp
  • A to G, chromosome 3 at 88,981,426 bp
  • T to C, chromosome 4 at 58,809,421 bp
  • C to A, chromosome 4 at 151,144,754 bp
  • A to G, chromosome 5 at 5,575,711 bp
  • A to G, chromosome 5 at 30,894,080 bp
  • G to A, chromosome 5 at 101,836,274 bp
  • A to G, chromosome 5 at 120,907,427 bp
  • T to C, chromosome 5 at 122,489,206 bp
  • C to T, chromosome 5 at 137,618,832 bp
  • C to A, chromosome 5 at 145,030,389 bp
  • A to G, chromosome 6 at 32,517,283 bp
  • T to C, chromosome 6 at 48,404,039 bp
  • T to C, chromosome 7 at 4,523,865 bp
  • T to C, chromosome 7 at 19,784,808 bp
  • A to G, chromosome 7 at 25,615,239 bp
  • A to T, chromosome 7 at 46,288,768 bp
  • G to T, chromosome 7 at 84,946,321 bp
  • A to G, chromosome 7 at 89,407,407 bp
  • GTGTCCTA to GTGTCCTATGTCCTA, chromosome 7 at 102,095,213 bp
  • A to G, chromosome 7 at 112,320,635 bp
  • T to A, chromosome 7 at 122,600,725 bp
  • A to C, chromosome 8 at 65,075,034 bp
  • T to C, chromosome 8 at 105,939,664 bp
  • A to T, chromosome 9 at 44,821,975 bp
  • A to G, chromosome 9 at 65,181,584 bp
  • A to T, chromosome 9 at 107,600,962 bp
  • GC to G, chromosome 10 at 59,892,752 bp
  • C to T, chromosome 10 at 115,119,874 bp
  • A to T, chromosome 11 at 28,576,768 bp
  • G to A, chromosome 11 at 75,433,205 bp
  • A to G, chromosome 11 at 109,953,514 bp
  • A to G, chromosome 11 at 121,513,732 bp
  • T to C, chromosome 13 at 78,024,999 bp
  • T to A, chromosome 14 at 31,258,439 bp
  • T to A, chromosome 14 at 77,091,679 bp
  • A to T, chromosome 16 at 20,607,780 bp
  • C to T, chromosome 17 at 24,698,317 bp
  • A to T, chromosome 18 at 36,999,650 bp
  • C to T, chromosome Y at 1,174,825 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5487 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043048-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.