Strain Detail Sheet

Strain Name:
B6J.129S7-Gt(ROSA)26Sorem1Rray/Mmucd
Stock Number:
043513-UCD
Citation ID:
RRID:MMRRC_043513-UCD
Other Names:
  • RC::FP_H2B-TagBFP_p2a_sfGFP_p2a_synaptophysin-tdTomato/Mmucd
  • Gt(ROSA)26Sortm#(CAG-H2B-TagBFP-sfGFPsynaptophysin-tdTomato)Rray 
  • RR5

Strain Information

Gene Details

Gt(ROSA)26Sorem1Rray
Name: gene trap ROSA 26, endonuclease-mediated mutation 1, Russell Ray
Type: Allele
Species: Multi-species
Chromosome: 6
CAG
Name: CMV early enhancer/chicken beta actin promoter/rabbit beta globin splice acceptor
Type: DNA Segment
Species: Multi-species
Chromosome:
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14910
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H2bc18
Name: H2B clustered histone 18
Synonyms: H2b-616, Hist2h2bb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 319189
Homologene: 136264
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Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Syp
Name: synaptophysin
Synonyms: Syp I, p38, Syn, A230093K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20977
Homologene: 2391
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
tdTomato
Name:
Type: Gene
Species: Discosoma sp. (mushroom coral)
Chromosome:
BFP
Name: blue fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
sfGFP
Name: superfolding green fluorescent protein
Type: Gene
Species: Aequorea victoria (jellyfish)
Chromosome:
Genetic Alterations
This line carries a Rosa26 knock-in of an intersectional cassette consisting of a CAG promoter, an FRT-flanked neomycin resistance gene with STOP cassette, a LoxP-flanked STOP cassette, and a cDNA of H2B-TagBFP, sfGFP, and synaptophysin-tdTomato separated by P2A self-cleaving peptide cassettes.
ES Cell Line
AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2

Strain Description

Phenotype
  • Homozygous: None expected.
  • Hetero/Hemizygous: None.
This mouse line is responsive to Cre and FLP recombinases. Upon Cre/FLP dual recombination, cells will express H2B-TagBFP (blue nuclei), sfGFP (green whole cell), and synaptophysin-tdTomato (red presynaptic contacts). It can be used to delineate cell types by dual expression of Cre and FLP recombinases through transgenic transmission or virus. Upon dual recombination, intersectional targeted cells will be marked with fluorescence in three areas of the cell: blue nuclei, green whole cell, and red presynaptic contacts. This is useful in studies on general neural morphology, circuit analysis, cell counts, and more.

Strain Development
  1. Generation of targeted AB2.2 ES cells through co-electroporation of CRISPR/Cas9 plasmid
  2. Injection of targeted AB2.2 ES cells into C57BL/6J blastocysts.
  3. Chimera breeding to C57BL/6J
  4. Offspring bred to C57BL/6J
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Cell Biology
  • Neurobiology
  • Research Tools

Strain Origin

Donor
Russell Ray, Ph.D., Baylor College of Medicine.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N5 (C57BL/6J)
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3 weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.