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Availability & Fees Order this Strain
Homozygous mutant mice appear normal. The strain donor observed reduced meiotic double-strand breaks at a meiotic recombination hot spot harboring insertion/deletion polymorphisms compared with controls.
A vector targeting exon 2 containing LoxP site - FRT site - PGK promoter - Neo cDNA - FRT - distal loxP was transfected into 129S2/SvEvPas-derived ES cells by genOway (France). Two clones were confirmed by PCR and Southern as correctly targeted at the Poln locus, injected into recipient C57BL/6 blastocysts, implanted into pseudo-pregnant females and offspring were assessed for recombinant ES cell contribution by using coat color markers. Two highly chimeric males were generated, mated with C57BL/6 Flp deleter females to allow germline excision of the neomycin selection cassette, which was confirmed by PCR. Progeny were bred a generation further with C57BL/6J mice to generate a pure line of excised floxed alleles, confirmed again by PCR screening and further verified by Southern blot. These mice represent the heterozygous conditional knockout model (B6;129Sv-Polntm1.1Rwd). Males were then mated to C57BL/6 Cre deleter females, offspring were assessed by PCR for excision of exon 2, further verified by Southern blotting. The resulting B6;129S6-Polntm1.2Rwd mouse line was backcrossed 4 times with C57BL/6J, and then random intrastrain-mated heterozygous to heterozygous, and then knockout to knockout for multiple generations.
Note: Recent MUGA analysis revealed 129S6 components.
Takata KI, Reh S, Yousefzadeh MJ, Zelazowski MJ, Bhetawal S, Trono D, LoweryMG, Sandoval M, Takata Y, Lu Y, Lin K, Shen J, Kusewitt DF, McBride KM, Cole F,Wood RD. Analysis of DNA polymerase ν function in meiotic recombination,immunoglobulin class-switching, and DNA damage tolerance. PLoS Genet. 2017 Jun1;13(6):e1006818. doi: 10.1371/journal.pgen.1006818. eCollection 2017 Jun. (Medline PMID: 28570559)
Colony Surveillance Program and Current Health Reports
The MMRRC at UC Davis performed microbita analysis on feces samples received from the donating investigator while the mice were still at their facility. There is a MMRRC Fecal Microbiota report available for this strain.If you are interested in having the MMRRC at UC Davis perform microbiota analysis once the line is established at your facility please contact mmrrc@ucdavis.edu.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.