Strain Detail Sheet

Strain Name:
B6J.B6-Tg(ACTB-NOX4)1Hcsa/Mmmh
Stock Number:
043684-MU
Citation ID:
RRID:MMRRC_043684-MU
Other Names:
lox-stop-lox Nox4 Tg, conditional human NADPH oxidase 4 overexpressor

Strain Information

Gene Details

Tg(ACTB-NOX4)1Hcsa
Name: transgene insertion 1, University of Texas Health Science Center at San Antonio
Type: Allele
Species: Homo sapiens (human)
Chromosome: unknown
Nox4
Name: NADPH oxidase 4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 50490
HGNC: HGNC:7891
Homologene: 41065
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
  • Transgene construct: 10415 bp in pGL3 backbone
  • hBeta-actin promoter: 7-3988 bp
  • Lox-STOP-Lox cassette: 4042-5529 bp (34 bp loxp sites are located at 4078-4111 & 5461-5494 bp)
  • hNox4 cDNA (ORF): 5546-7282 bp- SV40 late polyA: 7294-7554 bp
Genotype Determination

Strain Description

Phenotype

Homozygous: Not Characterized

Hetero/Hemizygous: No phenotype and no lethality before crossing with Cre.

Cre-excised Phenotype:

Strain Development
These mice have been maintained in their original background by backcrossing with C57BL/6J (Jax stock number 000664).
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Cardiovascular
  • Diabetes
  • Immunology and Inflammation
  • Models for Human Disease
  • Neurobiology

Strain Origin

Donor
Hanna E. Abboud, M.D., University of Texas Health Sciences Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
black
Other
available upon request

Breeding

MMRRC Breeding System
Backcross
Generation
>5
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3 weeks
Average Pups Weaned
4-6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.