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No distinguishable phenotypes presented as conditional KO mice phenotypes are just like wild-type mice, the phenotypes are only observed after Cre-mediated recombination.
Cre-reporter allele (R26mTmG) was bred into the Trp53f/f/Rb1f/f/Rbl2f/f mouse model of human small cell lung cancer (SCLC). The various strains were bred together to achieve quadruple conditional knockout mouse model. Briefly, the conditional alleles were combined in this order: ((Trp53 + Rb1) + Rbl2) + reporter; see publications listed below. Contributing background elements are 129P2, 129S4 (from ESCs), and C57BL/6 (chimera breeding) per submitter and MGI. The final effort of combining the alleles was not followed by backcrossing so these animals have to be deemed STOCK.
Vooijs M, van der Valk M, te Riele H, Berns A. Flp-mediated tissue-specificinactivation of the retinoblastoma tumor suppressor gene in the mouse. Oncogene. 1998 Jul 9;17(1):1-12. (Medline PMID: 9671308)
Marino S, Vooijs M, van Der Gulden H, Jonkers J, Berns A. Induction ofmedulloblastomas in p53-null mutant mice by somatic inactivation of Rb in theexternal granular layer cells of the cerebellum. Genes Dev. 2000 Apr15;14(8):994-1004. (Medline PMID: 10783170)
Meuwissen R, Linn SC, Linnoila RI, Zevenhoven J, Mooi WJ, Berns A. Inductionof small cell lung cancer by somatic inactivation of both Trp53 and Rb1 in aconditional mouse model. Cancer Cell. 2003 Sep;4(3):181-9. (Medline PMID: 14522252)
Schaffer BE, Park KS, Yiu G, Conklin JF, Lin C, Burkhart DL, Karnezis AN,Sweet-Cordero EA, Sage J. Loss of p130 accelerates tumor development in a mousemodel for human small-cell lung carcinoma. Cancer Res. 2010 May15;70(10):3877-83. doi: 10.1158/0008-5472.CAN-09-4228. Epub 2010 Apr 20. (Medline PMID: 20406986)
Denny SK, Yang D, Chuang CH, Brady JJ, Lim JS, Grüner BM, Chiou SH, Schep AN, Baral J, Hamard C, Antoine M, Wislez M, Kong CS, Connolly AJ, Park KS, Sage J,Greenleaf WJ, Winslow MM. Nfib Promotes Metastasis through a Widespread Increase in Chromatin Accessibility. Cell. 2016 Jul 14;166(2):328-342. doi:10.1016/j.cell.2016.05.052. Epub 2016 Jun 30. (Medline PMID: 27374332)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.