Strain Detail Sheet

Strain Name:
B6J.Cg-Y(IsXPAR;Y)Ei/EiJArnoMmucd
Stock Number:
043694-UCD
Citation ID:
RRID:MMRRC_043694-UCD
Other Names:
XY*, Y*; "Y star"

Strain Information

Gene Details

Y(IsXPAR;Y)Ei
Name: Chr Y, insertion of X PAR region to Y PAR region, Eva Eicher; Chr Y, insertion of X pseudoautosomal region to Y pseudoautosomal region, Eicher
Synonyms: Y star, T8Ei, T(XA1?;InY)8Ei, Y*
Type: Transposon
Species: Mus musculus (mouse)
Chromosome: Y
Alteration at locus: Insertion
Genetic Alterations
The Y* pseudoautosomal region (PAR) chromosome has resulted from an end-to-end fusion of an X and a Y PAR. It was shown that, in conjunction with this PAR-PAR fusion, there is a deletion of both copies of the distally located pseudoautosomal gene steroid sulfatase (Sts). The arrangement of the two PARs permits reciprocal translocation of X and Y chromatin at every meiotic event in the male, resulting in half of the sperm produced bearing one of two abnormal sex chromosomes.

The mutation was described in a preliminary fashion in Eicher, E M., "Primary sex-determining genes in mice." (1982). Faculty Research 1980 - 1989. 386, then investigated further in PMID:1743079.
Genotype Determination
Genotyping Protocol

Strain Description

Phenotype

Homozygous: Phenotypes extensively reported in publications cited. The model is useful for the study of any sex difference in a mouse phenotype.

MeSH Terms
  • Animals
  • Crossing Over, Genetic
  • Female
  • Gene Rearrangement
  • Male
  • Mice
  • Mice, Inbred C3H
  • X Chromosome
  • Y Chromosome
  • Base Sequence
  • Blotting, Southern
  • Centromere
  • Fertility/genetics
  • Fluorescence
  • Heterochromatin
  • Karyotyping
  • Mice, Inbred Strains
  • Mitosis/genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Recombination, Genetic/genetics
  • Sex Chromosome Aberrations/genetics
  • Cloning, Molecular
  • DNA/isolation & purification
  • DNA Probes
  • Electrophoresis, Agar Gel
  • Mice, Inbred C57BL
  • Multigene Family
  • Restriction Mapping
  • Retroviridae/genetics
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Central Nervous System/abnormalities
  • Central Nervous System/cytology
  • Central Nervous System/metabolism
  • Gene Deletion
  • Gene Dosage/genetics
  • Gene Expression Regulation, Developmental/genetics
  • Genes, sry/genetics
  • Mice, Knockout
  • Mutation/genetics
  • Neural Tube/abnormalities
  • Neural Tube/cytology
  • Neural Tube/metabolism
  • Neural Tube Defects/genetics
  • Neural Tube Defects/metabolism
  • Neural Tube Defects/physiopathology
  • Sex Characteristics
  • Sex Determination Processes
  • Sex Differentiation/genetics
  • Tumor Suppressor Protein p53/genetics
  • X Chromosome/genetics
  • X Chromosome Inactivation/genetics
  • Y Chromosome/genetics
  • Gene Expression Regulation/physiology
  • Genotype
  • Gonadal Steroid Hormones/metabolism
  • Sex Factors
  • Biomarkers/blood
  • Cholesterol, HDL/blood
  • Gene Dosage
  • Hypercholesterolemia/blood
  • Hypercholesterolemia/genetics
  • Orchiectomy
  • Ovariectomy
  • Ovary/metabolism
  • Phenotype
  • Testis/metabolism
  • Up-Regulation
  • Calcium/metabolism
  • Mitochondrial Membrane Transport Proteins
  • Myocardial Infarction/genetics
  • Myocardial Infarction/pathology
  • Myocardial Reperfusion Injury/genetics
  • Myocardial Reperfusion Injury/prevention & control
  • Adiposity/genetics
  • Body Composition/genetics
  • Body Temperature/genetics
  • Body Weight/genetics
  • Crosses, Genetic
  • Diet, High-Fat/adverse effects
  • Energy Metabolism/genetics
  • Glucose/metabolism
  • Metabolism/genetics
  • Models, Genetic
  • Sex Chromosome Aberrations
  • Adiposity/physiology
  • Diet, High-Fat
  • Gonadal Hormones/metabolism
  • Gonads/cytology
  • Gonads/metabolism
  • Insulin/blood
  • Lipid Metabolism/genetics
  • Lipids/blood
  • Obesity/genetics
  • Weight Gain/genetics
  • X Chromosome/physiology
  • Y Chromosome/physiology
  • Amygdala/metabolism
  • Behavior, Animal/physiology
  • Child
  • Gene Expression Regulation/genetics
  • Genes, X-Linked
  • Humans
  • Social Behavior
  • Turner Syndrome/blood
  • Turner Syndrome/genetics
  • Vasopressins/genetics
  • Vasopressins/metabolism
  • Disease Models, Animal
  • Sex Chromosomes/genetics
  • Aneuploidy
  • Brain/embryology
  • Brain/metabolism
  • Chromosome Breakpoints
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Genes, X-Linked/genetics
  • Genes, Y-Linked/genetics
  • Gonadal Steroid Hormones/genetics
  • Mice, Mutant Strains
  • Oligonucleotide Array Sequence Analysis
  • Transcription, Genetic
  • Translocation, Genetic
  • Aggression/physiology
  • Aromatase/biosynthesis
  • Copulation/physiology
  • Ejaculation/physiology
  • Estrogen Receptor alpha/biosynthesis
  • Genitalia, Male/anatomy & histology
  • Immunohistochemistry
  • Radioimmunoassay
  • Real-Time Polymerase Chain Reaction
  • Receptors, Androgen/biosynthesis
  • Septum of Brain/metabolism
  • Sexual Behavior, Animal/physiology
  • Vasopressins/blood
  • Behavior, Animal
  • Mice, Transgenic
  • Play and Playthings
  • Sex Chromosomes
  • Social Environment
Strain Development
B6Ei.LT-Y(IsXPAR;Y)Ei/EiJ (RRID:IMSR_JAX:002021) was backcrossed to C57BL/6J females for >14 generations. The Y* chromosome is backcrossed from the father.

This line is part of a group of related strains: MMRRC:43562, 43563, 43564, 43565, 43566, 43567, 43568, and 43694.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Internal_Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Reproduction
  • Research Tools
  • Sensorineural
  • Virology

Strain Origin

Donor
Arthur Arnold, Ph.D., University of California, Los Angeles.
Primary Reference

Burgoyne PS, Mahadevaiah SK, Perry J, Palmer SJ, Ashworth A. The Y*rearrangement in mice: new insights into a perplexing PAR. Cytogenet Cell Genet. 1998;80(1-4):37-40. (Medline PMID: 9678332)

Burgoyne PS, Arnold AP. A primer on the use of mouse models for identifyingdirect sex chromosome effects that cause sex differences in non-gonadal tissues. Biol Sex Differ. 2016 Dec 13;7:68. doi: 10.1186/s13293-016-0115-5. eCollection2016. Review. (Medline PMID: 27999654)

Eicher EM, Hale DW, Hunt PA, Lee BK, Tucker PK, King TR, Eppig JT, WashburnLL. The mouse Y* chromosome involves a complex rearrangement, includinginterstitial positioning of the pseudoautosomal region. Cytogenet Cell Genet.1991;57(4):221-30. (Medline PMID: 1743079)

Eicher EM, Hutchison KW, Phillips SJ, Tucker PK, Lee BK. A repeated segment onthe mouse Y chromosome is composed of retroviral-related, Y-enriched andY-specific sequences. Genetics. 1989 May;122(1):181-92. (Medline PMID: 2731728)

Chen X, Watkins R, Delot E, Reliene R, Schiestl RH, Burgoyne PS, Arnold AP.Sex difference in neural tube defects in p53-null mice is caused by differencesin the complement of X not Y genes. Dev Neurobiol. 2008 Feb 1;68(2):265-73.(Medline PMID: 18004765)

Arnold AP, Reue K, Eghbali M, Vilain E, Chen X, Ghahramani N, Itoh Y, Li J,Link JC, Ngun T, Williams-Burris SM. The importance of having two X chromosomes. Philos Trans R Soc Lond B Biol Sci. 2016 Feb 19;371(1688):20150113. doi:10.1098/rstb.2015.0113. Epub 2016 Feb 1. Review. (Medline PMID: 26833834)

Link JC, Chen X, Prien C, Borja MS, Hammerson B, Oda MN, Arnold AP, Reue K.Increased high-density lipoprotein cholesterol levels in mice with XX versus XYsex chromosomes. Arterioscler Thromb Vasc Biol. 2015 Aug;35(8):1778-86. doi:10.1161/ATVBAHA.115.305460. Epub 2015 Jun 25. (Medline PMID: 26112012)

Li J, Chen X, McClusky R, Ruiz-Sundstrom M, Itoh Y, Umar S, Arnold AP, EghbaliM. The number of X chromosomes influences protection from cardiacischaemia/reperfusion injury in mice: one X is better than two. Cardiovasc Res.2014 Jun 1;102(3):375-84. doi: 10.1093/cvr/cvu064. Epub 2014 Mar 19. (Medline PMID: 24654234)

Chen X, McClusky R, Itoh Y, Reue K, Arnold AP. X and Y chromosome complementinfluence adiposity and metabolism in mice. Endocrinology. 2013Mar;154(3):1092-104. doi: 10.1210/en.2012-2098. Epub 2013 Feb 8. (Medline PMID: 23397033)

Chen X, McClusky R, Chen J, Beaven SW, Tontonoz P, Arnold AP, Reue K. Thenumber of x chromosomes causes sex differences in adiposity in mice. PLoS Genet. 2012;8(5):e1002709. doi: 10.1371/journal.pgen.1002709. Epub 2012 May 10. (Medline PMID: 22589744)

Chen X, Watkins R, Delot E, Reliene R, Schiestl RH, Burgoyne PS, Arnold AP.Sex difference in neural tube defects in p53-null mice is caused by differencesin the complement of X not Y genes. Dev Neurobiol. 2008 Feb 1;68(2):265-73.(Medline PMID: 18004765)

Cox KH, Quinnies KM, Eschendroeder A, Didrick PM, Eugster EA, Rissman EF.Number of X-chromosome genes influences social behavior and vasopressin geneexpression in mice. Psychoneuroendocrinology. 2015 Jan;51:271-81. doi:10.1016/j.psyneuen.2014.10.010. Epub 2014 Oct 23. (Medline PMID: 25462900)

Cox KH, Bonthuis PJ, Rissman EF. Mouse model systems to study sex chromosomegenes and behavior: relevance to humans. Front Neuroendocrinol. 2014Oct;35(4):405-19. doi: 10.1016/j.yfrne.2013.12.004. Epub 2014 Jan 2. Review.(Medline PMID: 24388960)

Wolstenholme JT, Rissman EF, Bekiranov S. Sexual differentiation in thedeveloping mouse brain: contributions of sex chromosome genes. Genes Brain Behav.2013 Mar;12(2):166-80. doi: 10.1111/gbb.12010. Epub 2013 Jan 10. (Medline PMID: 23210685)

Bonthuis PJ, Cox KH, Rissman EF. X-chromosome dosage affects male sexualbehavior. Horm Behav. 2012 Apr;61(4):565-72. doi: 10.1016/j.yhbeh.2012.02.003.Epub 2012 Feb 10. (Medline PMID: 22349083)

Cox KH, Rissman EF. Sex differences in juvenile mouse social behavior areinfluenced by sex chromosomes and social context. Genes Brain Behav. 2011Jun;10(4):465-72. doi: 10.1111/j.1601-183X.2011.00688.x. Epub 2011 Apr 19. (Medline PMID: 21414140)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Microbiome Analysis

The MMRRC at UC Davis performed microbita analysis on feces samples received from the donating investigator while the mice were still at their facility. There is a MMRRC Fecal Microbiota report available for this strain.If you are interested in having the MMRRC at UC Davis perform microbiota analysis once the line is established at your facility please contact mmrrc@ucdavis.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Backcross
Breeding Scheme(s)
XY* males crossed with C57BL/6J (RRID:IMSR_JAX:000664 or first-generation XX daughters bred at UCLA) each generation.
Generation
N14+ (C57BL/6J)
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: N/A N/A
Hetero/Hemizygotes are fertile: N/A N/A
Age Reproductive Decline: Undetermined Greater than 12 months
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3-4 weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
043694-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit		 Aliquot Approximate quantity2 : 20-40 embryos / aliquot
043694-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit		 Aliquot Approximate quantity3
043694-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.