Strain Name:
STOCK Tbx6tm2Pa/Mmjax
Stock Number:
043813-JAX
Citation ID:
RRID:MMRRC_043813-JAX
Other Names:
Tbx6tm2(HIST1H2BB/EYFP)Pa, Tbx6tm2Pa; Tbx6 null EYFP knockin

Strain Information

Tbx6tm2Pa
Name: T-box 6; targeted mutation 2, Virginia Papaioannou
Synonyms: Tbx6tm2(HIST1H2BB/EYFP)Pa
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout
Tbx6
Name: T-box 6
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout
NCBI: 21389
Homologene: 3389
Genetic Alterations
This knock-out/knock-in EYFP reporter allele of Tbx6 (T-box 6) uses an EYFP fusion to delete exons 2 and part of 3, resulting in embryonic lethality by E9.5. Homozygous embryos develop randomized left/right axis patterning and altered morphology and motility in nodal cilia. These mice may be useful for studying left/right axis determination.
Genotype Determination
ES Cell Line
R1
Phenotype
The Tbx6 (T-box 6) gene belongs to a family of transcription factors involved in development and is crucial to specification of the posterior paraxial mesoderm and the extraembryonic allantois. Tbx6 alleles are associated with human spondylocostal dysostosis and scoliosis. These knock-out mice contain a human HIST1H2BB-EYFP fusion in frame with exon 1 and replacing exon 2 and part of exon 3. The region represents a portion of the T-box, a DNA binding domain. EYFP expression recapitulates Tbx6 expression. Homozygous embryos develop heterotaxia characterized by abnormal heart looping and randomized embryo turning. In addition, nodal cilia appears shortened, and thickened with terminal knobs and abnormal cilia motility is observed throughout the node, particularly in the pit of the node. Loss of asymmetric calcium signaling occurs at the periphery of the node. Somites develop ectopic neural tubes. Homozygous embryos die by E9.5 due to vascular hematomas. Heterozygotes are viable and fertile with minor skeletal abnormalities in the tail tip. These mice may be useful for studying left/right axis determination.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

Strain Development
A targeting vector containing a human HIST1H2BB-EYFP fusion in frame with the ATG in exon 1 and followed by loxP-flanked neomycin cassette was used to replace exon 2 and part of exon 3. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to ICR females. Offspring were maintained on a segregating background. Upon arrival, mice were bred to 129S1/SvImJ for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cardiovascular
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Virginia Papaioannou, Ph.D., Columbia University Medical Center.

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
segregating
MMRRC Breeding System
Outcross to non-inbred (hybrid or outbred) mate
Breeding Scheme(s)
Small colony with outcrosses to ICR.
Generation
NA
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Greater than 12 months Greater than 12 months
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3-4 weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043813-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
043813-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.