Strain Name:
B6J.B6N-Opn1mwtm1a(EUCOMM)Wtsi/WbaeMmmh
Stock Number:
044014-MU
Citation ID:
RRID:MMRRC_044014-MU
Other Names:
Opn1mw-/-

Strain Information

Opn1mwtm1a(EUCOMM)Wtsi
Name: opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan); targeted mutation 1a, Wellcome Trust Sanger Institute
Synonyms: Opn1mwGT
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
Opn1mw
Name: opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan)
Synonyms: Rsvp, green LWS cone opsin, green long wavelength sensitive cone opsin, Gcp, MWS opsin, Opn1lw, Midwavelength sensitive opsin, G/R opsin, ML-opsin, R/G opsin, Green opsin, M opsin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Targeted Mutation
NCBI: 14539
Homologene: 68064
Genetic Alterations
An ES cell line carrying the Opn1mw gene into which a 7122-bp gene trap was inserted in intron 2 (International Knockout Mouse Consortium, EUCOMM project).
ES Cell Line
JM8 derived from C57BL/6N
Phenotype
This mouse model has a deletion of the mouse middle-wavelength opsin gene (M-opsin knockout, or Opn1mw-/-). It has been used as a model for gene therapy study of human blue cone monochromacy, a severe vision disorder characterized by loss function of both L- and M-cones. These mice have no M-cone mediated electroretinogram responses but have a normal rod and S-cone function. The cone outer segments of the dorsal retina where M-opsin predominately expresses are significantly shorted, a phenotype resembling foveal cones of BCM patients.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Color Vision Defects/genetics
  • Color Vision Defects/metabolism
  • Color Vision Defects/pathology
  • Color Vision Defects/therapy
  • Dependovirus/genetics
  • Dependovirus/metabolism
  • Disease Models, Animal
  • Fovea Centralis/metabolism
  • Fovea Centralis/pathology
  • Gene Expression
  • Genetic Complementation Test
  • Genetic Therapy/methods
  • Genetic Vectors/chemistry
  • Genetic Vectors/metabolism
  • Humans
  • Mice
  • Mice, Knockout
  • Ophthalmoscopy
  • Promoter Regions, Genetic
  • Retinal Photoreceptor Cell Outer Segment/metabolism
  • Retinal Photoreceptor Cell Outer Segment/pathology
  • Rod Opsins/genetics
  • Rod Opsins/metabolism
  • Transgenes
Strain Development
An ES cell line carrying the Opn1mw gene into which a 7122-bp gene trap was inserted in intron 2 (International Knockout Mouse Consortium, EUCOMM project) was used to generate chimeras at the University of Utah transgenic core The gene trap consists of a splice acceptor sequence, an IRES (internal ribosome entry site) sequence, a promoterless lacZ gene, an SV40 termination signal, and a neomycin selection cassette. Proper location of the trap and the presence of 5′ and 3′ arms, as well as the location of loxP in intron 3, were confirmed by PCR and sequencing. Proper location of the trap and the presence of 5′ and 3′ arms, as well as the location of loxP in intron 3, were confirmed by PCR and sequencing. Animals were genotyped using primers GF6 (5′-TGGGTCCCTGAGGCTTGTGT), GR6 (5′-CCTCAACTTCAGCCTGCACACTTC) and LAR3B (5′-CAAGGGCAGTAGAGTTCCCAG). The WT allele yields an amplicon of 350 bp; mutant allele, 600 bp. Chimeric mice were outbred to C57BL/6J mice to generate Opn1mw+/− mice. Breeding Opn1mw+/GT− mice produced Opn1mwGT/GT− animals at Mendelian ratios.
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Models for Human Disease
Donor
Wolfgang Baehr, Ph.D., University of Utah
Primary Reference

Zhang Y, Deng WT, Du W, Zhu P, Li J, Xu F, Sun J, Gerstner CD, Baehr W, BoyeSL, Zhao C, Hauswirth WW, Pang JJ. Gene-based Therapy in a Mouse Model of BlueCone Monochromacy. Sci Rep. 2017 Jul 27;7(1):6690. doi:10.1038/s41598-017-06982-7. Erratum in: Sci Rep. 2018 Mar 14;8(1):4807. (Medline PMID: 28751656)

Deng WT, Li J, Zhu P, Chiodo VA, Smith WC, Freedman B, Baehr W, Pang J,Hauswirth WW. Human L- and M-opsins restore M-cone function in a mouse model for human blue cone monochromacy. Mol Vis. 2018 Jan 8;24:17-28. eCollection 2018.(Medline PMID: 29386880)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Outcross to non-inbred (hybrid or outbred) mate
Generation
N5+ (C57BL/6J)
Overall Breeding Performance
Excellent
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
4-6
Recommended wean age
3 Weeks
Average Pups Weaned
4-6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044014-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
044014-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.