Strain Name:
B6J.129/Sv-Selltm1Rpmc/Mmmh
Stock Number:
044018-MU
Citation ID:
RRID:MMRRC_044018-MU
Other Names:
L-selectinN138G^stop/stop

Strain Information

Selltm1Rpmc
Name: selectin, lymphocyte; targeted mutation 1, Rodger P McEver
Synonyms: Sell-
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knock-In
Sell
Name: selectin, lymphocyte
Synonyms: CD62L, LECAM-1, Ly-22, Lyam1, Lnhr, Lyam-1, L-selectin, Ly-m22
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Knock-In
NCBI: 20343
Homologene: 539
Genetic Alterations
In L-selectin, three nucleotides in the codon for Asn138 were changed to encode Gly138, and a neostop cassette was introduced into intron 2 before the mutation.
Genotype Determination
ES Cell Line
CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Phenotype
L-selectin on leukocyte surface forms flow-enhanced interactions termed "catch bonds" with its ligands, but without clear in vivo functions. We generated mice expressing an L-selectin mutant (N138G) that interacts with its ligands independently of catch bonds. We introduced a neo-stop cassette into intron 2, which prevents downstream transcription. In the presence of Cre, the floxed cassette is deleted, allowing expression of the mutated gene. Without Cre, this strain can serve as an L-selection knockout model.
Strain Development
We prepared murine embryonic stem (ES) cells with an allele encoding L-selectin p.Asn138Gly (N138G). Briefly, an Entrez Gene search of GenBank revealed an 18-kb intact Sell genomic sequence. The sequence around exon 4 was amplified by high-fidelity PCR using genomic DNA from CJ7 murine embryonic stem cells. These sequences were used to prepare a knockin targeting vector; site-directed mutagenesis was used to substitute three nucleotides in the codon for Asn138 so that it encoded Gly138. To avoid potentially fatal complications from the expression of the mutant gene during development, we introduced a loxP-flanked Neo-stop cassette fragment (a gift from Dr. Ioannis Dragatsis, University of Tennessee Health Science Center, Memphis, TN) into intron 2. The fidelity of the targeting construct was verified by DNA sequencing. The linearized targeting vector was electroporated into CJ7 murine ES cells. After G418 and ganciclovir selection, surviving clones with correct homologous recombination were identified by PCR and confirmed by Southern blotting and DNA sequencing. Heterozygous ES cells from one clone confirmed to have a normal karyotype were microinjected into C57BL/6J blastocysts to generate chimeric mice. Germline transmission was achieved from two chimeric males. The 138G mutation was identified by PCR amplification of a 323-bp fragment from mouse tail genomic DNA.
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Immunology and Inflammation
  • Research Tools
Donor
Rodger McEver, Ph.D Oklahoma Medical Research Foundation
Primary Reference

Liu Z, Yago T, Zhang N, Panicker SR, Wang Y, Yao L, Mehta-D'souza P, Xia L,Zhu C, McEver RP. L-selectin mechanochemistry restricts neutrophil priming invivo. Nat Commun. 2017 May 12;8:15196. doi: 10.1038/ncomms15196. (Medline PMID: 28497779)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N11 (C57BL6/J)
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 8 to 12 months
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044018-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
044018-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.