Strain Detail Sheet

Strain Name:
FVB.129P2(Cg)-Ptk2tm3.1Guan/Mmmh
Stock Number:
044020-MU
Citation ID:
RRID:MMRRC_044020-MU
Other Names:
FAK-PA

Strain Information

Gene Details

Ptk2tm3.1Guan
Name: PTK2 protein tyrosine kinase 2; targeted mutation 3.1, Jun-Lin Guan
Synonyms: FAK PA
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Targeted Mutation
Ptk2
Name: PTK2 protein tyrosine kinase 2
Synonyms: Fadk, FAK, FRNK
Type: Gene
Species: Mouse
Chromosome: 15
Alteration at locus: Targeted Mutation
NCBI: 14083
VEGA: 15
HGNC: HGNC:9611
Homologene: 7314
Genome Browser [Click image to go to Jbrowse]



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Genetic Alterations
Exon 28 was replaced with a floxed neo cassette and a modified exon 28 in which nucleotide substitution results in the amino acid substitution of alanine for proline at positions 878 and 881 (P878A, P881A). Cre-mediated recombination removed the neo cassette.

Note: At the time of development of the mouse, the available published data placed the PPRP motif at 878 to 881, but the numbering has since been refined and shifted; the corrected information is shown below:

  • gene: murine Ptk2 (NCBI: 14083)
  • mRNA RefSeq: NM_007982.2 (Isoform 1)
  • nucleic acid level: c.2626C>G and c.2635C>G
  • amino acid level, predicted: p.Pro876Ala (P876A) and p.Pro879Ala (P879A)
Genotype Determination
ES Cell Line
E14TG2a

Strain Description

Phenotype
Homozygous: no abnormal phenotype detected
MMTV-PyVT tumor model: decreased tumor size/growth, decreased metastatic potential
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Acyltransferases/metabolism
  • Animals
  • Biomarkers, Tumor/metabolism
  • Cell Movement
  • Cell Proliferation
  • Cell Transformation, Neoplastic/pathology
  • Epithelial-Mesenchymal Transition
  • Female
  • Focal Adhesion Kinase 1/metabolism
  • Gene Knock-In Techniques
  • Gene Knockdown Techniques
  • Humans
  • Lung Neoplasms/pathology
  • Lung Neoplasms/secondary
  • Mammary Neoplasms, Experimental/enzymology
  • Mammary Neoplasms, Experimental/pathology
  • Matrix Metalloproteinase 14/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Mutation/genetics
  • Neoplastic Stem Cells/enzymology
  • Neoplastic Stem Cells/pathology
  • Phosphorylation
  • Protein Binding
  • Spheroids, Cellular/metabolism
  • Spheroids, Cellular/pathology
  • Suppression, Genetic
  • Tumor Cells, Cultured
Strain Development
Gene targeting in 129P2/OlaHsd-derived E14Tg2a mouse embryonic stem cells was performed with the use of ESGRO. Chimeric mice were identified by coat color and then bred to C57BL/6J mice. Transmission of the germline was identified by PCR and confirmed by Southern blotting. Heterozygous targeted mice bearing PA[neo] allele (+/PA[neo] mice) were obtained and then crossed with EIIa-Cre mice, which express Cre in the very early stage of embryogenesis, to delete the neomycin cassette to avoid its possible interference with FAK gene expression. The progenies with neomycin cassette removed (+/PA; EIIa-Cre mice - JAX Strain #003724) were crossed with C57BL/6J mice to segregate the FAK PA allele from the heterozygote EIIa-Cre transgene. The resulting heterozygous and homozygous FAK P878A/P881A mutation knock-in mice (+/PA and PA/PA mice) were identified by PCR analysis and confirmed by sequencing of tail DNA.

The line is FVB congenic (N8) with additional components from 129P2 (due to the use of E14TG2a ES cells) and C57BL/6 from some initial breeding (not further specified).
Suggested Control Mice
FVB/N

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Cell Biology
  • Developmental Biology

Strain Origin

Donor
Jun-lin Guan, Ph.D University of Cincinnati
Primary Reference

Fan H, Zhao X, Sun S, Luo M, Guan JL. Function of focal adhesion kinasescaffolding to mediate endophilin A2 phosphorylation promotesepithelial-mesenchymal transition and mammary cancer stem cell activities invivo. J Biol Chem. 2013 Feb 1;288(5):3322-33. doi: 10.1074/jbc.M112.420497. Epub 2012 Dec 19. (Medline PMID: 23255596)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
White
Eye
Pink

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
N=8 (FVB)

F=>6
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Average litter size
7-9
Recommended wean age
3 Weeks
Average Pups Weaned
5-9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.