Strain Name:
C57BL/6J-MtgxR6230Btlr/Mmmh
Stock Number:
044359-MU
Citation ID:
RRID:MMRRC_044359-MU
Other Names:
R6230 (G1)
Major Collection:

Strain Information

Foxq1
Name: forkhead box Q1
Synonyms: HFH-1, Hfh1l, Hfh1, sa
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 15220
Homologene: 7359
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Arhgef12
Name: Rho guanine nucleotide exchange factor 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: MAST205, Mtssk
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17776
Homologene: 7428
Kif16b
Name: kinesin family member 16B
Synonyms: N-3 kinesin, 8430434E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16558
Homologene: 135708
Kntc1
Name: kinetochore associated 1
Synonyms: jgl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208628
Homologene: 32227
Atf1
Name: activating transcription factor 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11908
HGNC: HGNC:783
Homologene: 3790
Usp19
Name: ubiquitin specific peptidase 19
Synonyms: 8430421I07Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71472
Homologene: 41730
Focad
Name: focadhesin
Synonyms: BC057079
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230393
Homologene: 9842
Cttnbp2nl
Name: CTTNBP2 N-terminal like
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80281
Homologene: 36371
Parp4
Name: poly (ADP-ribose) polymerase family, member 4
Synonyms: VPARP, VAULT3, p193, PH5P, E230037B21Rik, Adprtl1, C030027K23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 328417
HGNC: HGNC:271
Homologene: 124423
Nf2
Name: neurofibromin 2
Synonyms: schwannomin, moesin-ezrin-radixin-like protein, merlin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18016
HGNC: HGNC:7773
Homologene: 2180
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Adnp
Name: activity-dependent neuroprotective protein
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11538
Homologene: 7617
Zfp160
Name: zinc finger protein 160
Synonyms: 6720480D16Rik, 6720480D16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224585
VEGA: 17
Homologene: 137372
Slitrk5
Name: SLIT and NTRK-like family, member 5
Synonyms: 2610019D03Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 75409
VEGA: 14
Homologene: 9193
Rnd2
Name: Rho family GTPase 2
Synonyms: Arhn, Rohn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11858
Homologene: 21123
Ppp1r2
Name: protein phosphatase 1, regulatory inhibitor subunit 2
Synonyms: 0610025N14Rik, 4930440J04Rik, 2310007G06Rik, 5430408E15Rik, I-2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66849
Homologene: 4546
Cspp1
Name: centrosome and spindle pole associated protein 1
Synonyms: 4930413O22Rik, 2310020J12Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 211660
Homologene: 23487
Vsir
Name: V-set immunoregulatory receptor
Synonyms: VISTA, Dies1, PD-1H, 4632428N05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74048
Homologene: 81923
Ext2
Name: exostosin glycosyltransferase 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14043
HGNC: HGNC:3513
Homologene: 345
Smurf2
Name: SMAD specific E3 ubiquitin protein ligase 2
Synonyms: 2810411E22Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66313
Homologene: 41490
Tars3
Name: threonyl-tRNA synthetase 3
Synonyms: A530046H20Rik, Tarsl2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 272396
Homologene: 65036
Zfp808
Name: zinc finger protein 808
Synonyms: Gm7036
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 630579
Homologene: 134631
Ttn
Name: titin
Synonyms: connectin, L56, 1100001C23Rik, mdm, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Col27a1
Name: collagen, type XXVII, alpha 1
Synonyms: 5730512J02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 373864
Homologene: 69400
Nlrp14
Name: NLR family, pyrin domain containing 14
Synonyms: 4921520L01Rik, Nalp14, GC-LRR, Nalp-iota
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76858
Homologene: 18531
Madd
Name: MAP-kinase activating death domain
Synonyms: 9630059K23Rik, Rab3 GEP
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228355
HGNC: HGNC:6766
Homologene: 14249
Or8b54
Name: olfactory receptor family 8 subfamily B member 54
Synonyms: GA_x6K02T2PVTD-32478047-32478988, MOR165-8, Olfr921
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258778
VEGA: 9
Homologene: 128138
Flg
Name: filaggrin
Synonyms: profilaggrin, fillagrin, ft
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14246
VEGA: 3
Klkb1
Name: kallikrein B, plasma 1
Synonyms: Kal-3, Kal3, Klk3, PSA
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16621
HGNC: HGNC:6371
Homologene: 68097
Shoc1
Name: shortage in chiasmata 1
Synonyms: LOC242489, Gm426, AI481877, Mzip2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100155
Homologene: 79783
Cyp2c39
Name: cytochrome P450, family 2, subfamily c, polypeptide 39
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13098
Homologene: 117948
Omg
Name: oligodendrocyte myelin glycoprotein
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18377
HGNC: HGNC:8135
Homologene: 36099
Rgs22
Name: regulator of G-protein signalling 22
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 626596
Homologene: 75047
Fn3krp
Name: fructosamine 3 kinase related protein
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 238024
Homologene: 11093
Or56b35
Name: olfactory receptor family 56 subfamily B member 35
Synonyms: GA_x6K02T2PBJ9-7942985-7943947, MOR40-3, Olfr689
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258745
Homologene: 128074
Nexn
Name: nexilin
Synonyms: nF actin binding protein, 1110046H09Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68810
Homologene: 44892
Musk
Name: muscle, skeletal, receptor tyrosine kinase
Synonyms: MDK4, Nsk1, Nsk2, Nsk3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18198
HGNC: HGNC:7525
Homologene: 4084
Dync2li1
Name: dynein cytoplasmic 2 light intermediate chain 1
Synonyms: mD2LIC, CGI-60, LIC3, 4933404O11Rik, D2lic
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 213575
VEGA: 17
Homologene: 9336
Usp16
Name: ubiquitin specific peptidase 16
Synonyms: 2810483I07Rik, UBP-M, 1200004E02Rik, 6330514E22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74112
Homologene: 38183
Vmn1r75
Name: vomeronasal 1 receptor 75
Synonyms: V1rg6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 171241
Homologene: 44234
Ccpg1
Name: cell cycle progression 1
Synonyms: 1700030B06Rik, 1810073J13Rik, D9Ertd392e, 9430028F23Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72278
Homologene: 3487
Rptn
Name: repetin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20129
Homologene: 84780
Tmem213
Name: transmembrane protein 213
Synonyms: D630002J15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 77522
Homologene: 28553
Fam174a
Name: family with sequence similarity 174, member A
Synonyms: 2310044D20Rik, Tmem157
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67698
Homologene: 12175
Atp23
Name: ATP23 metallopeptidase and ATP synthase assembly factor homolog
Synonyms: 1110068E08Rik, 2410012H02Rik, Xrcc6bp1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 68876
VEGA: 10
Homologene: 41699
Pdia6
Name: protein disulfide isomerase associated 6
Synonyms: CaBP5, P5, 1700015E05Rik, Txndc7
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 71853
Homologene: 4198
Gm21060
Name: predicted gene, 21060
Type: Gene
Species: Mouse
Chromosome: 19
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 10,077,197 bp
  • T to C, chromosome 1 at 95,314,226 bp
  • T to C, chromosome 2 at 59,842,031 bp
  • T to C, chromosome 2 at 76,919,434 bp
  • C to T, chromosome 2 at 91,143,521 bp
  • A to G, chromosome 2 at 93,762,620 bp
  • A to T, chromosome 2 at 142,849,912 bp
  • T to C, chromosome 2 at 168,182,532 bp
  • T to A, chromosome 3 at 93,279,475 bp
  • C to G, chromosome 3 at 93,398,130 bp
  • C to T, chromosome 3 at 105,011,339 bp
  • T to A, chromosome 3 at 152,238,275 bp
  • T to C, chromosome 4 at 58,367,576 bp
  • T to C, chromosome 4 at 59,099,345 bp
  • T to C, chromosome 4 at 63,224,282 bp
  • T to C, chromosome 4 at 88,342,204 bp
  • T to A, chromosome 4 at 101,837,214 bp
  • T to A, chromosome 4 at 116,326,098 bp
  • T to A, chromosome 5 at 123,789,009 bp
  • A to T, chromosome 6 at 38,114,616 bp
  • G to A, chromosome 7 at 11,881,039 bp
  • A to T, chromosome 7 at 65,686,436 bp
  • A to G, chromosome 7 at 105,314,082 bp
  • A to T, chromosome 7 at 107,181,817 bp
  • A to G, chromosome 8 at 45,283,215 bp
  • T to G, chromosome 9 at 38,775,777 bp
  • T to C, chromosome 9 at 42,988,965 bp
  • A to G, chromosome 9 at 73,012,356 bp
  • G to A, chromosome 9 at 108,501,941 bp
  • A to T, chromosome 10 at 60,358,078 bp
  • G to A, chromosome 10 at 126,887,562 bp
  • T to C, chromosome 11 at 4,808,262 bp
  • T to C, chromosome 11 at 79,502,958 bp
  • C to T, chromosome 11 at 101,468,999 bp
  • T to C, chromosome 11 at 106,868,504 bp
  • C to A, chromosome 11 at 121,425,592 bp
  • A to G, chromosome 12 at 17,277,213 bp
  • T to C, chromosome 13 at 11,660,107 bp
  • T to C, chromosome 13 at 31,559,508 bp
  • A to T, chromosome 13 at 62,172,322 bp
  • A to G, chromosome 14 at 56,607,533 bp
  • GACTAC to GACTACTAC, chromosome 14 at 111,679,816 bp
  • A to G, chromosome 15 at 36,100,030 bp
  • T to C, chromosome 15 at 100,232,824 bp
  • T to A, chromosome 16 at 31,260,600 bp
  • C to T, chromosome 16 at 87,464,798 bp
  • T to C, chromosome 17 at 21,026,445 bp
  • T to C, chromosome 17 at 84,647,650 bp
  • T to C, chromosome 19 at 39,536,802 bp
  • A to T, chromosome 19 at 61,297,011 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6230 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044359-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.