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Crabbe JC, Metten P, Rhodes JS, Yu CH, Brown LL, Phillips TJ, Finn DA. A line of mice selected for high blood ethanol concentrations shows drinking in the darkto intoxication. Biol Psychiatry. 2009 Apr 15;65(8):662-70. doi:10.1016/j.biopsych.2008.11.002. Epub 2008 Dec 18. (Medline PMID: 19095222)
Crabbe JC, Metten P, Belknap JK, Spence SE, Cameron AJ, Schlumbohm JP, HuangLC, Barkley-Levenson AM, Ford MM, Phillips TJ. Progress in a replicated selectionfor elevated blood ethanol concentrations in HDID mice. Genes Brain Behav. 2014Feb;13(2):236-46. doi: 10.1111/gbb.12105. Epub 2013 Dec 6. (Medline PMID: 24219304)
Ferguson LB, Ozburn AR, Ponomarev I, Metten P, Reilly M, Crabbe JC, Harris RA,Mayfield RD. Genome-Wide Expression Profiles Drive Discovery of Novel Compoundsthat Reduce Binge Drinking in Mice. Neuropsychopharmacology. 2018May;43(6):1257-1266. doi: 10.1038/npp.2017.301. Epub 2017 Dec 18. (Medline PMID: 29251283)
Crabbe JC, Metten P, Savarese AM, Ozburn AR, Schlumbohm JP, Spence SE, HackWR. Ethanol Conditioned Taste Aversion in High Drinking in the Dark Mice. BrainSci. 2019 Jan 1;9(1). pii: E2. doi: 10.3390/brainsci9010002. (Medline PMID: 30609665)
Crabbe JC, Ozburn AR, Metten P, Barkley-Levenson A, Schlumbohm JP, Spence SE, Hack WR, Huang LC. High Drinking in the Dark (HDID) mice are sensitive to theeffects of some clinically relevant drugs to reduce binge-like drinking.Pharmacol Biochem Behav. 2017 Sep;160:55-62. doi: 10.1016/j.pbb.2017.08.002. Epub2017 Aug 5. (Medline PMID: 28827047)
Hitzemann R, Oberbeck D, Iancu O, Darakjian P, McWeeney S, Spence S,Schlumbohm J, Metten P, Crabbe J. Alignment of the transcriptome with individual variation in animals selectively bred for High Drinking-In-the-Dark (HDID).Alcohol. 2017 May;60:115-120. doi: 10.1016/j.alcohol.2017.02.176. Epub 2017 Apr12. (Medline PMID: 28442218)
Colony Surveillance Program and Current Health Reports
This is the first selective breeding project that used a blood drug value for the selection index rather than behavior or physical characteristic. Furthermore, this unique genetic animal model drinks to higher blood ethanol concentrations than the standard inbred strain, C57BL/6J, that has been the highest preferring animal model. This first replicate (HDID-1), developed from a HS/Npt founder generation different than the one used for HDID-2 (MMRRC:65278 - PMID:25981501), shows different ethanol drinking microstructure and resistance to develop a conditioned taste aversion. Importantly, this drinking in the dark model has been adopted by many laboratories to mimic binge-like drinking in mice and has been adapted for other rodents (rats, voles, and hamsters). It is simple, minimally invasive, and does not require alcohol adulteration, thirst motivation, long periods of ramping up the ethanol concentration, administration by gavage, or other manipulations. The ethanol tube is placed on the cage of an individually housed mouse and the water is removed. When the drinking period is ended, water is restored. The mouse may choose whether or not to drink the ethanol.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.