Strain Name:
B6J;129P2-MffGt(AZ0438)Wtsi/DccMmucd
Stock Number:
066700-UCD
Citation ID:
RRID:MMRRC_066700-UCD
Other Names:
Mff gene trap

Strain Information

MffGt(AZ0438)Wtsi
Name: mitochondrial fission factor; gene trap AZ0438, Wellcome Trust Sanger Institute
Synonyms: Mffgt
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Gene Trap
Mff
Name: mitochondrial fission factor
Synonyms: 5230400G24Rik
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Gene Trap
NCBI: 75734
Homologene: 87000
Genetic Alterations
The mouse contains a gene trap at the Mff locus that abolishes protein expression
Genotype Determination
ES Cell Line
E14TG2a.4
Phenotype
Mff gene trap mice have dilated cardiomyopathy which results in death around 12-14 weeks. Mice are smaller and die prematurely with a mean life span of 13 weeks. The mice also suffer from malocclusion and kyphosis with incomplete penetrance. Mff mice also demonstrate neuromuscular defects, and both sexes have reduced fertility. Finally, the mice demonstrate an altered immunological profile with increased IgG deposits in tissues.
MeSH Terms
  • Animals
  • Cardiomyopathy, Dilated/metabolism
  • Cardiomyopathy, Dilated/pathology
  • Cells, Cultured
  • Female
  • Genetic Pleiotropy
  • Male
  • Membrane Proteins/deficiency
  • Membrane Proteins/genetics
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mitochondria, Heart/physiology
  • Mitochondrial Dynamics
  • Mitochondrial Proteins/deficiency
  • Mitochondrial Proteins/genetics
  • Titrimetry
Strain Development
Six male chimeras were derived from MMRRC ES cell line 020811-UCD with a background of 129P2/OlaHsd. The six males were backcrossed with female C57BL/6J mice (JAX Strain:000664). One of the chimeras produced 5 F1 heterozygotes (1 female, 4 males). Another chimera produced 20 F1 heterozygotes (8 females, 12 males). From the generated progeny, 7 females were mated with 8 males (at different times) to generate F2 mice. To maintain the colony, random intra-strain crosses of heterozygotes to WT and/or heterozygotes to heterozygote.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Immunology and Inflammation
  • Internal/Organ
  • Neurobiology
  • Reproduction
Donor
David Chan, M.D., California Institute of Technology.
Primary Reference

Chen H, Ren S, Clish C, Jain M, Mootha V, McCaffery JM, Chan DC. Titration of mitochondrial fusion rescues Mff-deficient cardiomyopathy. J Cell Biol. 2015 Nov 23;211(4):795-805. doi: 10.1083/jcb.201507035. (Medline PMID: 26598616)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Backcross and random intra-strain mating
Generation
N2 (C57BL/6J)
Overall Breeding Performance
Poor
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: Reduced Reduced
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Less than 4 months Less than 4 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
066700-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
066700-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.