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Availability & Fees Order this Strain
These mice carry an exon 13-18 deletion affecting the tyrosine kinase (TK) domain of the Ron receptor (macrophage stimulating 1 receptor; c-met-related tyrosine kinase) in the mouse Mst1r gene.
Mutant mice are overtly normal under standard housing conditions, but have altered inflammatory responses under a variety of different challenges. Slight developmental alterations in mammary gland development. Normal blood cell counts.
Development used E14TG2a ESCs derived from 129P2/OlaHsd. Targeted ES cells were transfected with the plasmid pML78, which contains a Cre recombinase cDNA with a nuclear localization signal driven by the human β-actin promoter and subsequently electroporated into blastocysts. Chimera and F1 were bred to NIH Black Suisse, and the resulting F2 heterozygotes were bred to homozygosity. Subsequent backcrossing to FVB/NJ to minimally the sixth generation with intermittent sib-mating.
Waltz SE, Eaton L, Toney-Earley K, Hess KA, Peace BE, Ihlendorf JR, Wang MH, Kaestner KH, Degen SJ. Ron-mediated cytoplasmic signaling is dispensable for viability but is required to limit inflammatory responses. J Clin Invest. 2001 Aug;108(4):567-76. doi: 10.1172/JCI11881. (Medline PMID: 11518730)
Leonis MA, Toney-Earley K, Degen SJ, Waltz SE. Deletion of the Ron receptor tyrosine kinase domain in mice provides protection from endotoxin-induced acute liver failure. Hepatology. 2002 Nov;36(5):1053-60. doi: 10.1053/jhep.2002.36822. (Medline PMID: 12395314)
Chan EL, Peace BE, Collins MH, Toney-Earley K, Waltz SE. Ron tyrosine kinase receptor regulates papilloma growth and malignant conversion in a murine model of skin carcinogenesis. Oncogene. 2005 Jan 13;24(3):479-88. doi: 10.1038/sj.onc.1208231. (Medline PMID: 15531916)
Stuart WD, Brown NE, Paluch AM, Waltz SE. Loss of Ron receptor signaling leads to reduced obesity, diabetic phenotypes and hepatic steatosis in response to high-fat diet in mice. Am J Physiol Endocrinol Metab. 2015 Apr 1;308(7):E562-72. doi: 10.1152/ajpendo.00467.2014. Epub 2015 Feb 3. (Medline PMID: 25648832)
Eyob H, Ekiz HA, Derose YS, Waltz SE, Williams MA, Welm AL. Inhibition of ron kinase blocks conversion of micrometastases to overt metastases by boosting antitumor immunity. Cancer Discov. 2013 Jul;3(7):751-60. doi: 10.1158/2159-8290.CD-12-0480. Epub 2013 Apr 23. (Medline PMID: 23612011)
Thobe MN, Gray JK, Gurusamy D, Paluch AM, Wagh PK, Pathrose P, Lentsch AB, Waltz SE. The Ron receptor promotes prostate tumor growth in the TRAMP mouse model. Oncogene. 2011 Dec 15;30(50):4990-8. doi: 10.1038/onc.2011.205. Epub 2011 May 30. (Medline PMID: 21625214)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Melissa Baines.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Melissa Baines
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.