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Mouse Foxn1 and EGFP (separated by an internal ribosome entry site) with a 3' CAG enhancer and lox-STOP-lox were inserted into the Rosa26 locus.
If without Cre or CreERT, homozygous FTg mice are normal. STOPflox-FoxN1 cDNA is driven by Rosa26 promoter. To obtain Foxn1 expression, crossing with a Cre or Cre-ERT gene-expressing line is necessary.
Conditional phenotype: When Cre-induced FoxN1 is expressed in prenatal life, the mice cannot survive. When mated to a CreERT line, tamoxifen-induced FoxN1 is expressed in early life (juvenile) mice, and homozygous mice either die or present with severe skin damage. However, if FoxN1 expression is induced (CreERT) in late life, there are benefits for rejuvenation age-related thymic involution. Using FoxN1 over-expressed fibroblasts via injection into the aged thymus, the donor could rejuvenate thymic involution and reduce self-reactive T cells induced chronic inflammation in aged mice.
The line was developed with an unspecified ES line derived from 129S1/SvImJ, with possible use of C57BL/6N-Tyrc-Brd/BrdCrCrl. Chimera mating details are unknown, but the line is reportedly backcrossed >6 times to C57BL/6NCrl.
Oh J, Wang W, Thomas R, Su DM. Thymic rejuvenation via FOXN1-reprogrammed embryonic fibroblasts (FREFs) to counteract age-related inflammation. JCI Insight. 2020 Sep 17;5(18):e140313. doi: 10.1172/jci.insight.140313. (Medline PMID: 32790650)
Ruan L, Zhang Z, Mu L, Burnley P, Wang L, Coder B, Zhuge Q, Su DM. Biological significance of FoxN1 gain-of-function mutations during T and B lymphopoiesis in juvenile mice. Cell Death Dis. 2014 Oct 9;5(10):e1457. doi: 10.1038/cddis.2014.432. (Medline PMID: 25299782)
Zhang Z, Burnley P, Coder B, Su DM. Insights on FoxN1 biological significance and usages of the "nude" mouse in studies of T-lymphopoiesis. Int J Biol Sci. 2012;8(8):1156-67. doi: 10.7150/ijbs.5033. Epub 2012 Sep 24. (Medline PMID: 23091413)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.